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R esearch
(Published online:
24-11-2015)
11. Histopathological and immunohistochemical diagnosis
of infectious bursal disease in poultry birds -
J. Singh, H. S. Banga, R. S. Brar, N. D. Singh, S. Sodhi and G. D.
Leishangthem
Veterinary World, 8(11): 1331-1339
doi:
10.14202/vetworld.2015.1331-1339
J. Singh:
Department of Veterinary Pathology, College of Veterinary
Science, Guru Angad Dev Veterinary and Animal Sciences
University, Ludhiana, Punjab, India;
js_arora23@yahoo.com
H. S. Banga:
Department of Veterinary Pathology, College of Veterinary
Science, Guru Angad Dev Veterinary and Animal Sciences
University, Ludhiana, Punjab, India;
bangahs3@yahoo.com
R. S. Brar:
Department of Veterinary Pathology, College of Veterinary
Science, Guru Angad Dev Veterinary and Animal Sciences
University, Ludhiana, Punjab, India;
rsbrar@email.com
N. D. Singh:
Department of Veterinary Pathology, College of Veterinary
Science, Guru Angad Dev Veterinary and Animal Sciences
University, Ludhiana, Punjab, India;
drndsingh@gmail.com
S. Sodhi:
Department of Veterinary Pathology, College of Veterinary
Science, Guru Angad Dev Veterinary and Animal Sciences
University, Ludhiana, Punjab, India;
sandip_sodhi@yahoo.com
G. D. Leishangthem: Department of Veterinary Pathology,
College of Veterinary Science, Guru Angad Dev Veterinary and
Animal Sciences
University, Ludhiana, Punjab, India;
drgeetapatho@gmail.com
Received: 27-06-2015, Revised: 04-10-2015, Accepted: 15-10-2015,
Published online: 24-11-2015
Corresponding author:
J. Singh, e-mail: js_arora23@yahoo.com
Citation:
Singh J, Banga HS, Brar RS, Singh ND, Sodhi S, Leishangthem GD
(2015) Histopathological and immunohistochemical diagnosis of
infectious bursal disease in poultry birds, Veterinary World
8(11): 1331-1339.
Abstract
Aim:
The aim of the present study was to diagnose infectious bursal
disease (IBD) using gross, histopathological, and
immunopathological approaches and to compare efficacy of
immunohistochemical techniques with conventional diagnostic
techniques.
Materials and Methods: A total of 33 samples were
collected from the six different poultry farms from Ludhiana and
the nearby districts. Upon gross analysis of the necropsied
birds, the relevant tissue samples such as bursa, kidney,
junction of proventriculus and gizzard, heart, and muscles were
then processed for histopathological and immunohistochemical
studies.
Results: Varied macroscopic changes were noted in bursa,
characterized as swollen, hemorrhages to atrophy in size.
Nonetheless, hemorrhages over thigh muscles were rarely seen.
Histologically, the bursa showed prominent fibrotic and atrophic
changes. Rarefaction of bursal follicles with intermittent
infiltration of lympho-mononuclear cells with chronic cystic
changes was additional changes, considered to be paramount for
IBD. Expression and localization of IBD specific viral antigens
were noticed mainly intracellular to the rarefied areas of
bursal follicle section(s), in conjunction to inner lining of
the cystic cavities of affected follicles. In addition, the
junction of proventriculus and gizzard, the heart muscle,
respiratory ciliated epithelium, and proventriculus also
revealed positive expression to IBD virus (IBDV) antigen.
Advanced immunopathological techniques, i.e., immunofluorescence
further testified the evidence of antigen as positive green
signal within affected follicles. Further consideration to the
reliability of various techniques employed, positive correlation
(r=0.64623) was emerged out with conventional pathological
scoring.
Conclusion: It is concluded that the bursa acts as an
organ of choice for demonstrating IBDV antigen for specific
diagnosis of disease using immunohistochemistry (IHC), and IHC
staining is a precise, specific, rapid, and reliable method to
demonstrate the IBDV antigen in the altered tissues due to IBDV
infection.
Keywords: histopathology, immunohistochemical, infectious
bursal disease.
References
|
1. Nyoni, N.M.B. and Masika, P.J. (2012) Village chicken
production practices in the amatola basin of the Eastern
Cape Province, South Africa. Afr. J. Agric. Res., 7:
2647-2652. |
|
|
|
2. Faragher, J.T. (1972) Infectious bursal disease of
chickens. Vet. Bull., 42: 361-369. |
|
|
3. Cosgrove, S.D. (1962) An apparently new disease of
chickens: Avian nephrosis. Avian Dis., 6: 385-389.
http://dx.doi.org/10.2307/1587909 |
|
|
|
4. Lukert, P.D. and Saif, Y.M. (2003) Infectious bursal
disease. Diseases of Poultry. Vol. 11. Iowa State University
Press, Ames, Iowa. p161-179. |
|
|
|
5. Haghighi, S.Z.M., Tavasoly, A., Shoshtary, A.,
Bahmaninejad, M.A. and Marjanmehr, S.H. (2009) An
experimental study on early pathogenesis of a very virulent
isolate of infectious bursal disease virus, employing
immunohistochemistry. Iran. J. Vet. Res., 10: 125-131. |
|
|
6. Ingrao, F., Rauw, F., Lambrecht, B. and Van Den Berg, T.
(2013) Infectious bursal disease: A complex host – Pathogen
interaction. Dev. Comp. Immunol., 41: 429-438.
http://dx.doi.org/10.1016/j.dci.2013.03.017
PMid:23567344 |
|
|
7. Uddin, M.M., Islam, M.S., Basu, J. and Khan, M.Z.I.
(2012) Distribution and quantification of lymphocytes in the
major lymphoid organs of naturally gumboro infected
broilers. Int. J. Morphol., 30: 1585-1589.
http://dx.doi.org/10.4067/S0717-95022012000400050 |
|
|
|
8. Musa, I.W., Saidu, L., Adamu, J., Mbuko, I.J., Kaltungo,
B.Y. and Abdu, P.A. (2010) Outbreaks of gumboro in growers
in Zaria, Nigeria. Niger. Vet. J., 31: 306-310. |
|
|
9. Farooq, M., Durrani, F.R., Imran, N., Durrani, Z. and
Chand, N. (2003) Prevalence and economic losses due to
infectious bursal disease in broilers in Mirpur and Kotli
districts of Kashmir. Int. J. Poult. Sci., 2: 267-270.
http://dx.doi.org/10.3923/ijps.2003.267.270 |
|
|
10. Jackwood, D.J. and. Sommer-Wagner, S.E. (2010) Detection
and characterization of infectious bursal disease viruses in
broilers at processing. Prev. Vet. Med., 97: 45-50.
http://dx.doi.org/10.1016/j.prevetmed.2010.07.010
PMid:20801535 |
|
|
11. Lawal, J.R., Jajere, S.M., Bello, A.M., Mustapha, M.,
Wakil, Y., Ndahi, J.J., Mustapha, F.B., Paul, B.T., Gulani,
I.A., Ibrahim, U.I., Geidam, Y.A., Ambali, A.G. and Waziri,
I. (2014) Prevalence of infectious bursal disease (Gumboro)
antibodies in village chickens in Gombe state, northeastern
Nigeria. Int. J. Poult. Sci., 13: 703-708.
http://dx.doi.org/10.3923/ijps.2014.703.708 |
|
|
12. Mbuko, I.J., Musa, W.I., Ibrahim, S., Saidu, L., Abdu,
P.A., Oladele, S.B. and Kazeem, H.M. (2010) A retrospective
analysis of infectious bursal disease diagnosed at poultry
unit of Ahmadu Bello University, Nigeria. Int. J. Poult. Sci.,
9: 784-790.
http://dx.doi.org/10.3923/ijps.2010.784.790 |
|
|
|
13. Anosa, G.N. and Eze, J.I. (2010) A sero-epidemiological
survey of infectious bursal disease in scavenging village
chickens in Enugu State. Niger. Vet. J., 3: 267-270. |
|
|
14. Musa, I.W., Saidu, L. and Abalaka, E.S. (2012) Economic
impact of recurrent outbreaks of gumboro disease in a
commercial poultry farm in Kano, Nigeria. Asian. J. Poult.
Sci., 6: 152-159.
http://dx.doi.org/10.3923/ajpsaj.2012.152.159 |
|
|
|
15. OIE. (2015) List. Available from: http://www.oie.int/en/animal-health-in-the-world/oie-listed-diseases-2015.
Accessed on 23-06-2015. |
|
|
|
16. Fantay, H., Balcha, E., Tesfay, A. and Afera, B. (2015)
Determining optimum time for administration of live
intermediate vaccine of infectious bursal disease to
chickens at Mekelle farm. J. Vet. Sci. Technol., 6: 223-227. |
|
|
17. Mahgoub, H.A. (2012) An overview of infectious bursal
disease. Arch. Virol., 157: 2047-2057.
http://dx.doi.org/10.1007/s00705-012-1377-9
PMid:22707044 |
|
|
18. Rekha, K., Sivasubramanian, C., Chung, I. and
Thiruvengadam, M. (2014) Growth and replication of
infectious bursal disease virus in the DF-1 cell line and
chicken embryo fibroblasts. Biomed. Res. Int., 2014: 6.
http://dx.doi.org/10.1155/2014/494835
PMid:24949455 PMCid:PMC4053150 |
|
|
19. Muller, H., Mundt, E., Eterradossi, N. and Islam, M.R.
(2012) Current status of vaccines against infectious bursal
disease. Avian Pathol., 41: 133-139.
http://dx.doi.org/10.1080/03079457.2012.661403
PMid:22515532 |
|
|
|
20. Bancroft, J.D. and Gamble, M. (2002) Theory and Practice
of Histological Techniques. 5th ed. Churchill Livingstone
Publisher, Edinburgh. p172-175, 593-620. |
|
|
21. Oladele, O.A., Adene, D.F., Obi, T.U. and Nottidge, H.O.
(2009) Comparative susceptibility of chickens, Turkeys and
ducks to infectious bursal disease virus using
immunohistochemistry. Vet. Res. Commun., 33: 111-121.
http://dx.doi.org/10.1007/s11259-008-9078-2
PMid:18663594 |
|
|
22. Juranova, R., Nga, N.T., Kulikova, L. and Jurajda, V.
(2001) Pathogenicity of Czech isolates of infectious bursal
disease virus. Acta Vet. Brno., 70: 425-431.
http://dx.doi.org/10.2754/avb200170040425 |
|
|
|
23. Khan, R.W., Khan, F.A., Khan, I. and Tariq, M. (2009)
Prevalence of infectious bursal disease in broiler in
District Peshawar. ARPN. J. Agric. Biol. Sci., 4: 1-5. |
|
|
|
24. Islam, M.T. and Samad, M.A. (2004) Clinico-pathological
studies on natural and experimental infectious bursal
disease in broiler chickens. Bangladesh J. Vet. Med., 2:
31-35. |
|
|
|
25. Rosenberger, J.K. (1989) Infectious bursal disease. In:
Purchase, H.G., Arp, L.R., Domermuth, C.H. and Pearson, J.E.,
editors. A Laboratory Manual for the Isolation and
Identification of Avian Pathogens. 3rd ed. AAAP, Kendall
Hunt Publishing Company, Dubuque, Iowa, USA. p165-166. |
|
|
26. Sharma, J.M., Dohms, J.E. and Metz, A.L. (1989)
Comparative pathogenesis of serotype 1 and variant serotype1
isolates of infectious bursal disease virus and their effect
on humoral and cellular immune competence of specific –
Pathogen-free chickens. Avian Dis., 33: 112-124.
http://dx.doi.org/10.2307/1591076
PMid:2539070 |
|
|
|
27. Sellaoui, S., Alloui, N., Mehenaoui, S. and Djaaba, S.
(2012) Evaluation of size and lesion scores of bursa cloacae
in broiler flocks in Algeria. J. World's Poult. Res., 2:
37-39. |
|
|
|
28. Guvenc, T., Hazıroglu, R., Yarım, M. and Tunca, R.
(2004) Diagnosis of infectious bursal disease by
immunoperoxidase technique Vet. J. Ankara Univ., 51:
237-238. |
|
|
29. Murmu, R., Islam, M.N., Most Juli, S.B., Khan, M.A.S.,
Harun-ur-Rashid, S.M., Hossain, F.M.A. and Rahman, M.M.
(2014) Pathogenicity and immunosuppressive properties of
GM-97 strain of infectious bursal disease virus in
commercial broiler chickens. J. Adv. Vet. Res., 1: 1-7.
http://dx.doi.org/10.5455/javar.v1i1p1-7 |
|
|
30. Hoque, M.M., Omar, A.R., Chong, L.K. and Hair-Bejoand
Aini, I. (2001) Pathogenicity of Sspl-positive infectious
bursal disease virus and molecular characterization of the
hypervariable region. Avian Pathol., 30: 369-380.
http://dx.doi.org/10.1080/03079450120066377
PMid:19184922 |
|
|
|
31. Islam, M.N., Rashid, S.M.H., Hoque, M.F., Juli, M.S.B.
and Khatun, M. (2008) Pathogenicity of IBDV related to
outbreaks in the vaccinated flocks and the causes of
vaccination failure. J. Innov. Dev. Strategy, 2: 22-30. |
|
|
|
32. Rudd, M., Heine, H., Parede, L., Sapats, S.I. and
Ignjatovic, J. (2001) Characterization of an Indonesian very
virulent strain of infectious bursal disease virus (IBDV).
Proceeding of the II International Symposium on Infectious
Bursal Disease and Chicken Infectious Anaemia, Held on 16-20
July, 2001, at Rauischholzhausen, Germany. p40-50. |
|
|
33. Ignjatovic, J., Sapats, S., Reece, R., Gould, A., Gould,
G., Selleck, P., Lowther, S. and Boyle, D. (2004) Westbury H
virus strains from a flock exhibiting unusually high
mortality due to infectious bursal disease. Aust. Vet. J.,
82: 763-768.
http://dx.doi.org/10.1111/j.1751-0813.2004.tb13244.x
PMid:15648939 |
|
|
34. Moraes, H.L.S., Salle, C.T.P., Nascimento, V.P., Salle,
F.O., Rocha, A.C.G.P., Souza, G.F., Furian, T.Q. and
Artencio, J.O. (2005) Infectious bursal disease: Evaluation
of maternal immunity and protection by vaccination of
one-day old chicks against challenge with a very virulent
virus isolate. Braz. J. Poult. Sci., 7: 51-57.
http://dx.doi.org/10.1590/s1516-635x2005000100009 |
|
|
35. Pantin-Jackwood, M.J. and Brown, T.P. (2003) Infectious
bursal disease virus and proventriculitis in broiler
chickens. Avian Dis., 47(3): 681-90.
http://dx.doi.org/10.1637/7018
PMid:14562897 |
|
|
36. Grau-Roma, L., Marco, A., Martinez, J., Chaves, A., Dolz,
R. and Majo, N. (2010) Infectious bursal disease-like virus
in cases of transmissible viral proventriculitis. Vet. Rec.,
167: 836.
http://dx.doi.org/10.1136/vr.c6561
PMid:21262640 |
|
|
|
37. Kumar, R. and Balachandran, C. (2009) Histopathological
changes in broiler chickens fed aflatoxin and cyclopiazonic
acid. Vet. Arch., 79: 31-40. |
|
|
38. Tippenhauer, M., Heller, D.E, Weigend, S. and
Reutenschlein, S. (2012) The host genotype influences
infectious bursal disease virus pathogenesis in chickens by
modulation of T cells responses and cytokine gene
expression. Dev. Comp. Immunol., 40: 1-10.
http://dx.doi.org/10.1016/j.dci.2012.10.013
PMid:23194926 |
|
|
39. Nunoya, T., Otaki, Y., Tajima, M., Hiraga, M. and Saito,
T. (1992) Occurrence of acute infectious bursal disease with
high mortality in Japan and pathogenicity of field isolates
in SPF chickens. Avian Dis., 36: 597-609.
http://dx.doi.org/10.2307/1591754
PMid:1329709 |
|
|
|
40. Tanimura, N., Tsukamoto, K., Nakamura, K., Narita, M.
and Yuasa, N. (1994) Pathological changes in
specific-pathogen-free chickens experimentally inoculated
with European and Japanese highly virulent strains of
infectious bursal disease virus. In: Proceedings of the
International Symposium on infectious bursal disease and
chicken infectious anaemia. World Veterinary Poultry
Association, Germany. p143-152. |
|
|
41. Jonsson, L.G.O. and Engstrom, B.E. (1986)
Immunohistochemical detection of infectious bursal disease
and infectious bronchitis viral antigens in fixed,
paraffin-embedded chicken tissues. Avian Pathol., 15:
385-393.
http://dx.doi.org/10.1080/03079458608436301
PMid:18766540 |
|
|
|
42. Hemalatha, S., Murali Manohar, B. and Balachandran, C.
(2009) Sequential immunohistochemical detection of
infectious bursal disease virus antigen in experimental
chicken. Indian J. Vet. Pathol., 33: 82-84. |
|
|
43. Van den Berg, T.P., Gonze, M. and Meulemans, G. (1991)
Acute infectious bursal disease in poultry: Isolation and
characterisation of a highly virulent strain. Avian Pathol.,
20: 133-143.
http://dx.doi.org/10.1080/03079459108418748 |
|
|
|
44. Islam, M.T., Islam, M.N., Khan, M.Z.I. and Islam, M.A.
(2011) Comparison of agar gel immunodiffusion test,
immunohistochemsitry and reverse transcription - Polymerase
chain reaction for detection of infectious bursal disease
virus. Bangladesh J. Vet. Med., 9: 121-125. |
|
