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R esearch
(Published online:
05-11-2015)
2. Cross antigenicity of immunodominant polypeptides of
somatic antigen of Oesophagostomum columbianum with other
helminths by western blotting - Sunita Dalal, Arvind
Prasad, Abdul Nasir and Vijesh Kumar Saini
Veterinary World, 8(11): 1279-1285
doi:
10.14202/vetworld.2015.1279-1285
Sunita Dalal:
Network Program on G.I. Parasitism, Division of Parasitology,
Indian Veterinary Research Institute, Izatnagar, Bareilly,
Uttar Pradesh, India;
drsunita009@gmail.com
Arvind Prasad:
Network Program on G.I. Parasitism, Division of Parasitology,
Indian Veterinary Research Institute, Izatnagar, Bareilly,
Uttar Pradesh, India;
drarvindivri@rediffmail.com
Abdul Nasir:
Network Program on G.I. Parasitism, Division of Parasitology,
Indian Veterinary Research Institute, Izatnagar, Bareilly,
Uttar Pradesh, India;
drnasirivri@rediffmail.com
Vijesh Kumar Saini: Network Program on G.I. Parasitism,
Division of Parasitology, Indian Veterinary Research Institute,
Izatnagar, Bareilly,
Uttar Pradesh, India;
vijesh24@gmail.com
Received: 20-06-2015, Revised: 17-09-2015, Accepted: 24-09-2015,
Published online: 05-11-2015
Corresponding author:
Arvind Prasad, e-mail: drarvindivri@rediffmail.com
Citation:
Dalal S, Prasad A, Nasir A, Saini VK (2015) Cross antigenicity of
immunodominant polypeptides of the somatic antigen of
Oesophagostomum columbianum with other helminths by western
blotting, Veterinary World 8(11): 1279-1285.
Abstract
Aim:
Oesophagostomum columbianum
in small ruminants in India is found as mixed infection commonly
in sheep and goat. Haemonchus contortus, an abomasal
nematode is found as concurrent infection with it. Eggs of
Haemonchus and O. columbianum cannot be easily
distinguished. Diagnosis of O. columbianum may only be
possible if a non-cross antigenic polypeptide was available for
immunodiagnosis.
Materials and Methods:
Somatic antigen (SoAg) of O. columbianum was fractionated
by sodium dodecyl sulfatepolyacrylamide gel electrophoresis and
immunodominant polypeptides were identified by western blotting
with homologous hyperimmune serum (HIS) and experimental sera of
sheep or goat infected with other helminths.
Results:
SoAg of O. columbianum was immunoaffinity purified. Sharp
polypeptide bands of 130, 72 and 68 KDa were observed along with
several faint bands of lower molecular weight. Western blot of
purified SoAg of O. columbianum with homologous HIS
showed reaction with all the protein bands of 17, 28, 30, 32,
35, 38, 50, 68, 100, 130, 150, and 170 kDa. For identification
of non-cross antigenic polypeptide, immunoaffinity purified SoAg
of O. columbianum was reacted to heterologous HIS against
H. contortus, Paramphistomum epiclitum, and Fasciola
gigantica in western blotting utilizing completely dry
method (i-blot). Among high molecular weight polypeptides 100
and 150 kDa were non-cross antigenic and among low molecular
weight except 50 kDa polypeptide, 17, 30, 32, 35, and 38 kDa of
O. columbianum were not cross antigenic with other
helminths.
Conclusions:
Hence, polypeptides of 17, 30, 32, 35 and 38 kDa as well as 100
and 150 kDa polypeptides of O. columbianum may be
exploited for immunodiagnosis of the infection in sheep and goat
with extensive studies on cross antigenicity.
Keywords:
cross antigenicity, hyperimmune sera, immunodiagnostic
polypeptides, Oesophagostomum columbianum.
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