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R esearch
(Published online:
21-11-2015)
7.
Comparative efficacy of different estrus
synchronization protocols on estrus induction response,
fertility and plasma progesterone and biochemical profile in
crossbred anestrus cows -
A. J. Dhami, B. B. Nakrani, K. K. Hadiya, J. A. Patel and R. G.
Shah
Veterinary World, 8(11): 1310-1316
doi:
10.14202/vetworld.2015.1310-1316
A. J. Dhami:
Department of Animal Reproduction, Gynaecology and Obstetrics,
College of Veterinary Science and Animal Husbandry, Anand
Agricultural University, Anand-388 001, Gujarat, India;
ajdhami@aau.in
B. B. Nakrani:
Department of Animal Reproduction, Gynaecology and Obstetrics,
College of Veterinary Science and Animal Husbandry, Anand
Agricultural University, Anand-388 001, Gujarat, India;
bhaveshnakrani@sagbidaj.org
K. K. Hadiya:
Department of Animal Reproduction, Gynaecology and Obstetrics,
College of Veterinary Science and Animal Husbandry, Anand
Agricultural University, Anand-388 001, Gujarat, India;
kamleshhadiya@yahoo.co.in
J. A. Patel:
Department of Animal Reproduction, Gynaecology and Obstetrics,
College of Veterinary Science and Animal Husbandry, Anand
Agricultural University, Anand-388 001, Gujarat, India;
japatel@aau.in
R. G. Shah:
Department of Animal Reproduction, Gynaecology and Obstetrics,
College of Veterinary Science and Animal Husbandry, Anand
Agricultural University, Anand-388 001, Gujarat, India;
rohitshah_44@yahoo.co.in
Received: 15-06-2015, Revised: 24-09-2015, Accepted: 05-10-2015,
Published online: 21-11-2015
Corresponding author:
A. J. Dhami, e-mail: ajdhami@aau.in
Citation:
Dhami AJ, Nakrani BB, Hadiya KK, Patel JA, Shah RG (2015)
Comparative efficacy of different estrus synchronization
protocols on estrus induction response, fertility and plasma
progesterone and biochemical profile in crossbred anestrus cows,
Veterinary World 8(11):
1310-1316.
Abstract
Aim:
To evaluate estrus induction response and fertility including
plasma progesterone and biochemical profile following use of
three standard hormonal protocols in anestrus crossbred cows.
Materials and Methods:
The study was carried out on 40 true anestrus and 10 normal
cyclic cows. 10 anestrus cows each were treated with standard
intravaginal controlled internal drug release (CIDR) device,
Ovsynch (GPG) protocol, and Norgestomet ear implant with
fixed-time artificial insemination (FTAI). 10 anestrus cows were
kept as untreated control while 10 cows exhibiting the first
estrus within 90 days postpartum without any treatment served as
normal cyclic control. Blood samples were obtained from treated
cows on day 0, 7, 9 (AI) of treatment and day 21 post-AI, and
from control groups on the day of AI and day 21 post-AI for
estimation of plasma progesterone, protein, cholesterol,
calcium, and inorganic phosphorus profile.
Results:
The use of CIDR, Ovsynch, and Norgestomet ear implant protocols
resulted in 100% estrus induction with conception rates at
induced estrus of 60%, 50%, and 50%, and the overall of three
cycles as 80%, 80%, and 70%. In untreated anestrus control
(n=10), only three cows exhibited spontaneous estrus within 90
days of follow-up and conceived giving the first service and
overall conception rates of 66.66% and 30.00%, respectively. In
normal cyclic control (n=10), the conception rates at first and
overall of three cycles were 50% and 80%. The overall mean
plasma progesterone (P4) concentrations in anestrus cows studied
on day 0 (initiation), 7 (prostaglandin injection and/or removal
of implant), 9 (FTAI) of treatment and on day 21 post-AI
revealed that the values on day 7 and 21 were significantly
(p<0.01) higher than other two periods in all three groups. The
concentrations were significantly (p<0.05) higher in conceived
than non-conceived group on day 21 post-AI in CIDR (4.36±0.12
vs. 1.65±0.82 ng/ml) and Ovsynch (4.85±0.62 vs. 1.59±0.34 ng/ml),
but not in Norgestomet ear implant (4.50±0.53 vs. 3.02±1.15 ng/ml)
or normal cyclic group (5.39±0.67 vs. 3.13±0.37 ng/ml). The
cholesterol and protein levels were significantly higher, but
not the calcium and phosphorus, in normal cyclic control than in
anestrus groups. The influence of treatment days and pregnancy
status was not significant for any of the biochemical
constituents in any of the groups.
Conclusion:
Ovsynch and/or CIDR synchronization protocol can be effectively
used to improve fertility up to 80% in anestrus cows, as
compared to 30% in anestrus control, combined with plasma
progesterone to delineate the reproductive status before and
after treatment.
Keywords:
anestrus, conception rate, cow, estrus induction, fertile estrus
induction interval,treatment protocols.
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