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R esearch
(Published online:
28-10-2015)
15. Conventional and
fluorescent based semen quality assessment in Karan Fries bulls
- A. Panmei, A. K. Gupta, P. R. Shivahre,
M. Bhakat and A. Upadhyay
Veterinary World, 8(10): 1243-1246
doi:
10.14202/vetworld.2015.1243-1246
A.
Panmei:
Division of Dairy Cattle Breeding, Indian Council of Agricultural
Research, National Dairy Research Institute, Karnal,
Haryana, India; panmei.achun07@gmail.com
A.
K. Gupta:
Division of Dairy Cattle Breeding, Indian Council of Agricultural
Research, National Dairy Research Institute, Karnal,
Haryana, India; guptaak2009@gmail.com
P.
R. Shivahre:
Division of Dairy Cattle Breeding, Indian Council of Agricultural
Research, National Dairy Research Institute, Karnal,
Haryana, India; drpr06@gmail.com
M.
Bhakat:
Division of Dairy Cattle Breeding, Indian Council of Agricultural
Research, National Dairy Research Institute, Karnal,
Haryana, India; bhakat.mukesh@gmail.com
A.
Upadhyay: Division of Dairy Cattle Breeding, Indian Council of
Agricultural Research, National Dairy Research Institute, Karnal,
Haryana, India;
upadhyay.arpan@gmail.com
Received: 30-05-2015, Revised: 10-09-2015, Accepted:
18-09-2015, Published online: 28-10-2015
Corresponding author:
A. Panmei, e-mail: panmei.achun07@gmail.com
Citation:
Panmei A, Gupta AK,
Shivahre PR, Bhakat M, Upadhyay A (2015) Conventional and
fluorescent based semen quality assessment in Karan Fries bulls,
Veterinary World 8(10): 1243-1246.
Abstract
Aim:
The present study was carried out on semen ejaculates of 15
Karan Fries (KF) bulls maintained at Artificial Breeding Research
Centre, National Dairy Research Institute, Karnal, India with an
objective to evaluate the relationship between the conventional
and fluorescent based semen quality analysis of the bulls.
Materials and Methods: A total of 96 ejaculates were collected
from 15 KF (Holstein Friesian [HF] crossbred) bulls. Semen were
evaluated for color, volume, mass activity (MA) and percentage of
individual motility (IM), sperm concentration, percent live
spermatozoa, hypo-osmotic swelling test and acrosome integrity,
chromatin integrity, sperm viability, and membrane integrity. Data
were analyzed using SPSS software package for descriptive
analysis. The correlation between rankings of sires based on
conventional and fluorescent semen parameters were calculated by
Spearman’s rank correlation coefficient.
Results: The average ejaculates volume (ml), sperm
concentration (10 6/ml), MA, IM (%), live
(%), morphological abnormalities (%), host (%), acrosome integrity
(%), chromomycin A3 (CMA3) (%), SYBR-PI (%), and fluorescent
isothiocyanate-peanut agglutinin (FITC-PNA) (%) were 4.57±0.36,
1162.98±97.93, 2.95±0.09, 60.8±1.22, 71.41±2.10, 9.31±1.15,
65.5±1.81, 86.6±1.59, 3.53±0.43, 65.39±2.23 and 74.47±2.53,
respectively. Rank correlations were found to be significant for
SYBR-PI and FITC-PNA with most of the parameters evaluated by
conventional methods. Overall, among conventional criteria, IM
revealed ranking of bulls almost similar to that of fluorescent
criteria.
Conclusion: Overview of our results
indicated that, among conventional criteria, MA and IM revealed
ranking of bulls almost similar to that of fluorescent criteria.
Keywords: conventional method, fluorescent
technique, Karan Fries, semen quality.
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