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R esearch
(Published online:
10-09-2015)
4.
Phenotypic and genotypic detection of virulence factors of
Staphylococcus aureus isolated from clinical and subclinical
mastitis in cattle and water buffaloes from different farms of
Sadat City in Egypt -
Mohamed Sabry Elsayed,
Abd Elrahman Mahmoud El-Bagoury and Mai Abdallah Dawoud
Veterinary World, 8(9): 1051-1058
doi:
10.14202/vetworld.2015.1051-1058
Mohamed Sabry Elsayed:
epartment of Bacteriology, Mycology and Immunology Faculty of
Veterinary Medicine University of Sadat City,
Menoufia 32897, Egypt; Mohamed.sabry@vet.usc.edu.eg
Abd
Elrahman Mahmoud El-Bagoury:
Department of Food Hygiene and Control, Faculty of Veterinary
Medicine University of Sadat
City,
Menoufia, 32897, Egypt; elbagory200@yahoo.com
Mai
Abdallah Dawoud: Library of Faculty of Veterinary Medicine,
University of Sadat City, Menoufia, 32897, Egypt;
mai_dawoud30@yahoo.com
Received: 07-05-2015, Revised: 27-07-2015, Accepted: 07-08-2015,
Published online: 10-09-2015
Corresponding author:
Mohamed Sabry Elsayed, e-mail: Mohamed.sabry@vet.usc.edu.eg
Citation:
Elsayed MS, El-Bagoury
AM, Dawoud MA (2015) Phenotypic and genotypic detection of
virulence factors of Staphylococcus aureus isolated from
clinical and subclinical mastitis in cattle and water buffaloes
from different farms of Sadat City in Egypt, Veterinary World 8(8):1051-1058.
Abstract
Aim:
To characterize
Staphylococcus aureus
from clinical and subclinical mastitis
and identify virulence factors.
Materials and Methods: Two
hundred and two milk samples were collected, 143 from mastitic
cattle and buffaloes 94 and 49, respectively, and 59 from
apparently healthy cattle and buffaloes 35 and 24, respectively.
Results:
California mastitis test was applied and positive prevalence were
91.48% and 75.51% for cattle and buffalo with clinical mastitis
and 37.14% and 45.83% for cattle and buffalo with subclinical
mastitis. S. aureus
was isolated from clinically mastitic
cattle and buffaloes were 39.29% and 50%, respectively. While,
from subclinical mastitic cattle and buffaloes were 80% and
72.73%, respectively. Hemolytic activity was assessed for
S. aureus
isolated from clinically and subclinical mastitic cases with
prevalences of 100% and 56.25%, respectively. Thermo nuclease
production from clinically and subclinical mastitic cases was 25%
and 56.25%, respectively. Simplex polymerase chain reaction (PCR)
conducted on S. aureus
using 16S rRNA, clumping factor A, Panton
valentine leukocidin, coagulase (Coa), alphahemolysin and beta-hemolysin
those proved existence in 100%, 46.9%, 65.6%, 100%, 34.4%, and
43.75% of the isolates, respectively. While, multiplex PCR is
utilized for detection of enterotoxins and proved that 12.5% was
positive for enterotoxine Type D.
Conclusions: It is concluded
that simplex and multiplex PCR assays can be used as rapid and
sensitive diagnostic tools to detect the presence of
S. aureus
and characterize its virulence factors that help in detection of
severity of infection, distribution and stating preventive and
control strategies.
Keywords: clinical and
subclinical mastitis, enterotoxins, identification,
Staphylococcus aureus,
polymerase chain reaction, virulence factors.
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