Open Access
Research
(Published
online: 30-04-2016)
16.
Comparative study on
immunoglobulin Y transfer from breeding hens to egg yolk and
progeny chicks in different breeds of poultry -
Ritu Agrawal, S. D. Hirpurkar, C. Sannat and Amit Kumar Gupta
Veterinary World, 9(4): 425-431
doi:
10.14202/vetworld.2016.425-431
Ritu Agrawal:
Department
of Veterinary Microbiology, College of Veterinary Science &
Animal Husbandry, Anjora, Durg, Chhattisgarh, India; ritu2808@rediffmail.com
S. D. Hirpurkar:
Department of Veterinary Microbiology, College of Veterinary
Science & Animal Husbandry, Anjora, Durg, Chhattisgarh, India;
smpuhir@yahoo.com
C. Sannat:
Department
of Veterinary Microbiology, College of Veterinary Science &
Animal Husbandry, Anjora, Durg, Chhattisgarh, India; csannat@rediffmail.com
Amit Kumar Gupta:
Department
of Veterinary Microbiology, College of Veterinary Science &
Animal Husbandry, Anjora, Durg, Chhattisgarh, India;
dramitkumaragrahari2009@gmail.com
Received: 16-12-2015, Accepted: 19-03-2016, Published online:
30-04-2016
Corresponding author:
C. Sannat, e-mail: csannat@rediffmail.com
Citation:
Agrawal R, Hirpurkar SD, Sannat C, Gupta AK (2016) Comparative
study on immunoglobulin Y transfer from breeding hens to egg
yolk and progeny chicks in different breeds of poultry,
Veterinary World, 9(4): 425-431.
Abstract
Aim:
This study
was undertaken to compare the immunoglobulin Y (IgY) level and
its efficacy in laying hens of four different breeds of poultry
(viz.,Vanraja, Gramapriya, BlackRock, and KalingaBrown)
and its relative transfer in egg yolk and chick.
Materials and
Methods:
This study was
conducted in 48 apparently healthy laying hens vaccinated with
Salmonella inactivated polyvalent vaccine, eggs and
progeny chicks; 12 each from four different breeds of poultry,viz.,Vanraja,
Gramapriya, BlackRock, and KalingaBrown. The methodology
included measurement of egg and yolk weight, total protein and
IgY in egg yolk, total serum protein and IgY in breeding hens,
and progeny chicks and extent of IgY transfer from hens to yolk
then to chicks. Further, Salmonella-specific antibodies
in breeding hens, egg yolk and progeny chicks were assessed
using O and H antigen by tube agglutination test.
Results:
The
egg weight differed nonsignificantly (p>0.05) among breeds,
however, breed wise significant variation (p<0.01) was reported
in yolk weight. The weight of egg yolk significantly affects the
total protein and IgY concentration although these levels per
unit of volume did not differ. Total protein was significantly
higher (p<0.01) in KalingaBrown and Gramapriya as compared to
Vanraja and BlackRock. Non-significant (p>0.05) difference among
breed was found in total protein of egg yolk and chick. The IgY
concentration in hens, egg yolk and chick was found to be in the
range of 5.35±0.63- 5.83±0.65, 2.3±0.1-2.6±0.2, and
1.3±0.11-1.7±0.16 mg/ml, respectively which is uniform and
independent of total protein concentration at all the three
levels. Significant breed variations were not observed in
maternal IgY transfer from breeding hens to chicks and were
25.62±1.42-36.06±4.34% of total IgY in parent flock. Moderate to
higher rate of seroprevalence with peak titers of 1:640 against
Salmonella-specific antibodies was observed in only 41.6%
of breeding hens.
Conclusion:
No
significant difference in the rate of transfer of IgY was
observed in four breeds studied (viz.,Vanraja, Gramapriya,
BlackRock, and KalingaBrown) and moderate seropositivity was
detected for Salmonella-specific antibodies in progeny
chicks.
Keywords:
breeding hens, chicks, maternal immunoglobulin Y, Salmonella
antibody, yolk.
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