Open Access
Research
(Published
online: 30-08-2016)
21.
Genotypic study of verocytotoxic
Escherichia coli
isolates from deer by multiplex polymerase
chain reaction -
Raghavendra Prasad Mishra, Udit Jain and Rakesh Kumar Singh
Veterinary World, 9(8): 919-921
doi:
10.14202/vetworld.2016.919-921
Raghavendra Prasad Mishra:
Department of Veterinary Public Health, Veterinary University,
UP Pandit Deen Dayal Upadhyaya Veterinary Science University,
Mathura, Uttar Pradesh, India; rmishra523@rediffmail.com
Udit Jain:
Department of Veterinary Public Health, Veterinary University,
UP Pandit Deen Dayal Upadhyaya Veterinary Science University,
Mathura, Uttar Pradesh, India; druditjain@hotmail.com
Rakesh Kumar Singh:
Veterinary Officer, Kanpur Zoo, Kanpur, Uttar Pradesh, India;
rksinghalldup@yahoo.com
Received: 15-04-2016, Accepted: 22-07-2016, Published online:
30-08-2016
Corresponding author:
Raghavendra Prasad Mishra, e-mail: rmishra523@rediffmail.com
Citation:
Mishra RP, Jain U, Singh RK (2016) Genotypic study of
verocytotoxic
Escherichia coli
isolates from deer by multiplex polymerase chain reaction,
Veterinary World, 9(8):
919-921.
Abstract
Aim:
This study was planned to study the genotypes of
verocytotoxigenic
Escherichia coli
(VTEC) in fecal samples of deer due to its public health
significance.
Materials and Methods:
A total of 160 fecal samples of deer were taken from Mathura
district and Kanpur Zoo and screened for VTEC genes by
polymerase chain reaction (PCR).
Results:
All fecal samples were positive for
E. coli.
All the
E. coli
isolates were screened by PCR to detect virulence genes
stx1,
stx2,
eaeA,
and
hlyA.
Of these, 15 isolates were found positive for VTEC having one or
more genes in different combinations.
Conclusion:
Genes such as
stx1,
stx2,
eaeA,
and
hlyA
were prevalent in VTEC isolates from feces of deer. The presence
of VTEC isolates having virulent genes may pose a threat to
public health.
Keywords:
deer, polymerase chain reaction, verocytotoxic
Escherichia coli.
References
1. Kumar, A., Verma, A.K., Sharma, A.K. and Rahal, A. (2013)
Presence of extended spectum beta-lactamases producing alpha
hemolytic Escherichia coli in yellow-wattled lapwing
(Vanellus malabaricus). Asian J. Anim. Sci., 7(2): 64-69.
http://dx.doi.org/10.3923/ajas.2013.64.69 |
|
|
|
2. Mailk, S., Kumar, A., Verma, A.K., Gupta, M.K., Sharma,
S.D., Sharma, A.K. and Rahal, A. (2013) Incidence and drug
resistance pattern of collibacillosis in cattle and buffalo
calves in Western Uttar Pradesh in India. J. Anim. Health
Prod., 1(1): 15-19. |
|
|
3. Dhama, K., Rajagunalan, S., Chakraborty, S., Verma, A.K.,
Kumar, A., Tiwari, R. and Kapoor, S. (2013) Food-borne
pathogens of animal origin diagnosis, prevention and control
and their zoonotic significance - A review. Pak. J. Biol.
Sci., 16(20): 1076-1085.
http://dx.doi.org/10.3923/pjbs.2013.1076.1085 |
|
|
4. Kumar, A., Verma, A.K., Malik, S., Gupta, M.K., Sharma,
A. and Rahal, A. (2014) Occurrence of extended spectrum
beta-lactamases producing alpha hemolytic Escherichia coli
in Neonatal diarrhea. Pak. J. Biol. Sci., 17(1): 109-113.
http://dx.doi.org/10.3923/pjbs.2014.109.113
PMid:24783787 |
|
|
|
5. Anita., Kumar, A., Verma, A.K., Gupta, M.K. and Rahal, A.
(2014) Multi drug resistant pathogenic Escherichia coli in
water sources and Yamuna river in and around Mathura, India.
Pak. J. Biol. Sci., 17(4): 540-544. |
|
|
6. O'Brien, A.D. and Holmes, R.K. (1987) Shiga and
Shiga-like toxins. Microbiol. Rev., 51: 206-220.
PMid:3299029 PMCid:PMC373104 |
|
|
7. Hiruta, N., Murase, T. and Okamura, N. (2001) An outbreak
of diarrhea due to multiple antimicrobial resistant Shiga
toxin producine E. coli O26:h7 ina nursery. Epidemiol.
Infect., 127: 221-227.
http://dx.doi.org/10.1017/S0950268801006069
PMid:11693499 PMCid:PMC2869741 |
|
|
8. Croxen, M.A. and Finlay, B.B. (2010) Molecular mechanisms
of Escherichia coli pathogenicity. Nat. Rev. Microbiol., 8:
26-38.
PMid:19966814 |
|
|
9. Gyles, C.M. and Fairbrother, J.M. (2010) Escherichia
coli. In: Gyles, C.L., Prescott, J.F. and Thoen, C.O.,
editors. Pathogenesis of Bacterial Infections in Animals.
Blackwell Publishing, Ames. p267-308.
http://dx.doi.org/10.1002/9780470958209.ch15 |
|
|
10. Sanchez, S., Sanchez, D.S., Martinez, R., Llorente, M.T.,
Herrera-Leon, S. and Vidal, D. (2013) The new allelic
variantof the subtilase cytotoxin (sub AB2) is common among
Shiga toxin producing Escherichia coli strains from large
animals and their meat and meat products. Vet. Microbiol.,
166: 645-649.
http://dx.doi.org/10.1016/j.vetmic.2013.06.031
PMid:23880308 |
|
|
11. Diaz, S., Vidal, D., Herrera-Leon, S. and Sanchez, S.
(2011) Sorbitol fermenting, b-glucuronidase-positive, Shiga
toxin negative Escherichia coli O157:H7 in free-ranging red
deer in South-Central Spain. Foodborne Pathog. Dis., 8:
1313-1315.
http://dx.doi.org/10.1089/fpd.2011.0923
PMid:21819212 |
|
|
12. Sanchez, S., Martnez, R., Garca, A., Vidal, D., Blanco,
J., Blanco, M., Blanco, J.E., Mora, A., Herrera-Leon, S.,
Echeita, A., Alonso, J.M. and Rey, J. (2010) Detection and
characterization of O157:H7 and non-O157 Shiga toxin
producing Escherichia coli in wild boars. Vet. Microbiol.,
143: 420-423.
http://dx.doi.org/10.1016/j.vetmic.2009.11.016
PMid:20005055 |
|
|
|
13. OIE. (2004) Verocytotoxigenic Escherichia coli. In: Word
Organization for Animal Health, Pairs Manual of Diagnostic
Tests and Vaccines for Terrestrial Animals. 5th ed. OIE,
Paris. |
|
|
|
14. Ewing, W.H. (1986) The genus Escherichia. In: Edwards,
P.R. and Ewing, W.H., editors. Edwards and Ewing's
Identification of Enterobacteriaceae. 4th ed. Elsevier
Science Publishing Co. Inc., New York. p93-122. |
|
|
15. Paton, A.W. and Paton, J.C. (1998) Detection and
characterization of Shiga toxigenic Escherichia coli by
using multiplex PCR assays for stx1, stx2, eaeA,
enterohemorrhagic E. coli hlyA, rfb O111, and rfb O157. J.
Clin. Microbiol., 36(2): 598-602.
PMid:9466788 PMCid:PMC104589 |
|
|
16. Bardiau, M., Gregoire, F., Muylaert, A., Nahayo, A.,
Duprez, J.N., Mainil, J. and Linden, A. (2010)
Enteropathogenic (EPEC), enterohaemorragic (EHEC) and
verotoxigenic (VTEC) Escherichia coli in wild cervids. J.
Appl. Microbiol., 109(6): 2214-2222.
http://dx.doi.org/10.1111/j.1365-2672.2010.04855.x
PMid:20880133 |
|
|
17. Sanchez, S., Martínez, R., Rey, J., García, A., Blanco,
J., Blanco, M., Blanco, J.E., Mora, A., Herrera-León, S.,
Echeita, A. and Alonso, J.M. (2010) Pheno-genotypic
characterisation of Escherichia coli O157:H7 isolates from
domestic and wild ruminants. Vet. Microbiol., 142(3-4):
445-449.
http://dx.doi.org/10.1016/j.vetmic.2009.10.009
PMid:19914011 |
|
|
|
18. Hiroshi, A., Sou-ichi, M., Toshikazu, S., Teizo, T.,
Hisao, K., Tetsuya, I. and Kouichi, T. (2013) Detection and
genetic characterization of Shiga toxin-producing
Escherichia coli from Wild Deer. Microbiol. Immunol.,
42(12): 815-822. |
|
|
|
19. Fukuyama, M., Yokoyama, R., Sakata, S., Furuhata, K. and
Oonaka, K. (1999) Study on the verotoxic producing
Escherichia coli isolation of the bacteria from deer dung.
J. Jpn. Assoc. Infect. Dis., 73(11): 1140-1144. |
|
|
20. Sa’nchez, S., Garcı’a-Sa’nchez, A., Martı’nez, R.,
Blanco, J., Blanco, J.E., Blanco, B., Dahbi, G., Mora, A.,
Hermoso, J., Mendoza, D., Alonso, J.M. and Rey, J. (2009)
Detection and characterisation of Shiga toxin-producing
Escherichia coli other than Escherichia coli O157:H7 in wild
ruminants. Vet. J., 180: 384-388.
http://dx.doi.org/10.1016/j.tvjl.2008.01.011
PMid:18337133 |
|
|
21. Leotta, G.A., Deza, N., Origlia, J., Toma, C., Chinen,
I., Miliwebsky, E., Iyoda, S., Sosa-Estani, S. and Rivas, M.
(2006) Detection and characterization of Shiga toxin
producing Escherichia coli in captive nondomestic mammals.
Vet. Microbiol., 118: 151-157.
http://dx.doi.org/10.1016/j.vetmic.2006.07.006
PMid:16919898 |
|
|
22. Beutin, L., Geier, D., Zimmerman, S. and Karch, H.
(1995) Virulence markers of Shiga-like toxin-producing E.
coli strains from healthy domestic animals of different
species. J. Clin. Microbiol., 33: 631-635.
PMid:7538509 PMCid:PMC228004 |
|
|
23. Chapman, P.A. and Siddons, C.A. (1997) A comparison of
immunomagnetic separation and direct culture for the
isolation of verocytotoxin-producing Escherichia coli O157
from cases of bloody diarrhoea, non-bloody diarrhoea and
asymptomatic contacts. J. Med. Microbiol., 44: 267-271.
http://dx.doi.org/10.1099/00222615-44-4-267
PMid:8606354 |
|
|
|
24. Johnson, R.P., Clarke, R.C. and Gyles, C.L. (1996)
Growing concerns and recent outbreaks involving non-O157:H7
serotypes of verotoxigenic Escherichia coli. J. Food Prot.,
59: 1112-1122. |
|
|
25. Schouten, J.M., van de Giessen, A.W., Frankena, K., De
Jong, M.C.M. and Graat, E.A.M. (2005) Escherichia coli O157
prevalence in dutch poultry, pig finishing and veal herds
and risk factors in dutch veal herds. Prev. Vet. Med., 70:
1-15.
http://dx.doi.org/10.1016/j.prevetmed.2004.12.010
PMid:15951037 |
|
|
26. Oporto, B., Esteban, J.I., Aduriz, G., Juste, R.A. and
Hurtado, A. (2008) Escherichia coli O157:H7 and non-O157
Shiga toxin-producing E. coli in healthy cattle, sheep and
Swine herds in Northern Spain. Zoonoses Public Health,
55(2): 73-81.
http://dx.doi.org/10.1111/j.1863-2378.2007.01080.x
PMid:18234025 |
|
