Open Access
Research
(Published
online: 17-12-2016)
16.
Phenotypic and genotypic characterization of locally isolated
Salmonella
strains used in preparation of
Salmonella
antigens in Egypt -
Hazem Mohammed Ibrahim, Dalia Ahmed Mohammed Abd El-moaty, Hanan Ali
Ahmed and Mona Ibrahim El-Enbaawy
Veterinary World, 9(12): 1435-1439
doi:
10.14202/vetworld.2016.1435-1439
Hazem Mohammed Ibrahim:
Department of Bacterial Sera and Antigens Research, Veterinary
Serum and Vaccine Research Institute, Abbasia, Cairo, Egypt;
dr.hazemibrahim@gmail.com
Dalia Ahmed Mohammed Abd El-moaty:
Genetic Engineering Research Department, Veterinary Serum and
Vaccine Research Institute, Abbasia, Cairo, Egypt; dody.ahmed@gmail.com
Hanan Ali Ahmed:
Central Laboratory for Evaluation of Veterinary Biologics, Cairo,
Egypt; vetehana@yahoo.com
Mona Ibrahim El-Enbaawy:
Department of Microbiology, Faculty of Veterinary Medicine, Cairo
University, Cairo, Egypt; dr_mona_1@hotmail.com
Received: 16-08-2016, Accepted: 09-11-2016, Published online:
17-12-2016
Corresponding author:
Hazem Mohammed Ibrahim, e-mail: dr.hazemibrahim@gmail.com
Citation:
Ibrahim HM, Abd El-moaty DAM, Ahmed HA, El-Enbaawy MI (2016)
Phenotypic and genotypic characterization of locally isolated
Salmonella
strains used in preparation of
Salmonella
antigens in Egypt,
Veterinary World, 9(12):
1435-1439.
Abstract
Aim:
This work was conducted to study the phenotypic and genotypic
characterization of locally isolated
Salmonella
strains (Salmonella
Pullorum,
Salmonella
Enteritidis, and
Salmonella
Typhimurium) from poultry used in the preparation of
Salmonella
antigens in Egypt.
Materials and Methods:
The phenotypic characterization of
Salmonella
strains was done using standard microbiological, biochemical, and
serological techniques. Molecular identification was done using
different sets of primers on different genes using different
polymerase chain reaction (PCR) techniques.
Results:
The phenotypic characterization of
Salmonella
strains was confirmed. Molecular identification revealed detection
of 284 bp fragment of InvA gene in all studied
Salmonella
strains. Furthermore, multiplex PCR was used for more confirmation
of being
Salmonella
spp., generally at 429 bp as well as genotyping of
Salmonella
Typhimurium and
Salmonella
Enteritidis at 559 and 312 bp, respectively, in one reaction.
Conclusion:
The locally isolated field
Salmonella
strains were confirmed phenotypically and genotypically to be
Salmonella
Enteritidis, and
Salmonella
Typhimurium and could be used for the preparation of
Salmonella
antigens.
Keywords:
characterization, duplex polymerase chain reaction, multiplex
polymerase chain reaction,
Salmonella
spp.
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