Open Access
Research
(Published
online: 22-12-2016)
22.
Estrus induction and fertility response
following different treatment protocols in Murrah buffaloes under
field conditions -
L. Kumar, J. B. Phogat, A. K. Pandey, S. K. Phulia, S. Kumar and
J. Dalal
Veterinary World, 9(12): 1466-1470
doi:
10.14202/vetworld.2016.1466-1470
L. Kumar:
Department of Veterinary Gynaecology and Obstetrics, College of
Veterinary Sciences, Lala Lajpat Rai University of Veterinary
and Animal Sciences, Hisar - 125 004, Haryana, India;
dr.lokesh24@yahoo.com
J. B. Phogat:
Department of Veterinary Gynaecology and Obstetrics, College of
Veterinary Sciences, Lala Lajpat Rai University of Veterinary and
Animal Sciences, Hisar - 125 004, Haryana, India; phogat_jagat@rediffmail.com
A. K. Pandey:
Department of Veterinary Gynaecology and Obstetrics, Teaching
Veterinary Clinical Complex, College of Veterinary Sciences, Lala
Lajpat Rai University of Veterinary and Animal Sciences, Hisar -
125 004, Haryana, India; dranandpandey@gmail.com
S. K. Phulia:
Department of Animal Physiology and Reproduction, ICAR-Central
Institute for Research on Buffalo, Hisar - 125 004, Haryana,
India; sphulia@gmail.com
S. Kumar:
Department of Veterinary Gynaecology and Obstetrics, College of
Veterinary Sciences, Lala Lajpat Rai University of Veterinary and
Animal Sciences, Hisar - 125 004, Haryana, India;
sandeeppanihar48@gmail.com
J. Dalal:
Department of Veterinary Gynaecology and Obstetrics, College of
Veterinary Sciences, Lala Lajpat Rai University of Veterinary and
Animal Sciences, Hisar - 125 004, Haryana, India; jasmer.sl.dalal@gmail.com
Received: 18-06-2016, Accepted: 19-11-2016, Published online:
22-12-2016
Corresponding author:
A. K. Pandey, e-mail: dranandpandey@gmail.com
Citation:
Kumar L, Phogat JB, Pandey AK, Phulia SK, Kumar S, Dalal J (2016)
Estrus induction and fertility response following different
treatment protocols in Murrah buffaloes under field conditions,
Veterinary World, 9(12):
1466-1470.
Abstract
Aim:
The aim of this study was to evaluate the efficacy of three
different treatment protocols for estrus induction and conception
rate in postpartum anestrus buffaloes during breeding season under
field conditions.
Materials and Methods:
The 47 postpartum anestrus buffaloes of the 2nd to 6th parity were
divided into three groups. Group 1 (n=16): Buffaloes received
cosynch treatment, that is, buserelin acetate 10 μg on day 0 and
9, cloprostenol 500 μg on day 7 followed by fixed-time artificial
insemination (FTAI) at the time of second buserelin acetate and 24
h later. Group 2 (n=15): Buffaloes received norgestomet ear
implant subcutaneously for 9 days, estradiol benzoate 2 mg on the
day of implant insertion (day 0), pregnant mare serum gonadotropin
(PMSG) 400 IU and cloprostenol 500 μg on day 9 followed by AI at
48 and 72 h after implant removal. Group 3 (Cosynch-plus, n=16):
Buffaloes received Cosynch protocol as per Group 1 except an
additional injection of PMSG 400 IU (i.m.) was given 3 days before
the start of protocol and FTAI done at the same time of Group 1.
Pregnancy diagnosis was performed after 45 days of AI.
Results:
The estrus induction response following the treatment was 81.3%,
100%, and 93.7% in Group 1, 2, and 3, respectively. The buffaloes
of Group 1, 2, and 3 expressed intense (38.4%, 60% and 46.6%,
respectively) and moderate estrus (46.1%, 26.6%, and 40%,
respectively). The conception rates in Group 1, 2, and 3, at FTAI
and overall including subsequent estrus were 37.5% and 62.5%,
53.3%, and 66.6%, 56.3%, and 75%, respectively.
Conclusion:
All the three treatment protocols can be effectively used for
induction of estrus with acceptable conception rate in postpartum
anestrus buffaloes during breeding season under field conditions.
However, Cosynch-plus (similar to Cosynch protocol except addition
of PMSG, 400 IU 3 days before the start of first buserelin acetate
administration) protocol results comparatively better pregnancy
rate.
Keywords:
anestrus, Cosynch-plus, estrus induction, norgestomet.
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