Open Access
Research
(Published
online: 03-12-2016)
3.
Ocimum sanctum
Linn. stimulate the expression of choline acetyltransferase on the
human cerebral microvascular endothelial cells -
Dwi Liliek Kusindarta, Hevi Wihadmadyatami
and Aris Haryanto
Veterinary World, 9(12): 1348-1354
doi:
10.14202/vetworld.2016.1348-1354
Dwi Liliek Kusindarta :
Department of
Anatomy, Faculty of Veterinary Medicine, Universitas Gadjah Mada,
Yogyakarta, Indonesia; indarta@ugm.ac.id
Hevi Wihadmadyatami :
Department of
Anatomy, Faculty of Veterinary Medicine, Universitas Gadjah Mada,
Yogyakarta, Indonesia; heviwihadmadyatami@ugm.ac.id
Aris Haryanto :
Department of
Biochemistry, Faculty of Veterinary Medicine, Universitas Gadjah
Mada, Yogyakarta, Indonesia; arisharyanto@yahoo.com
Received: 12-06-2016, Accepted: 13-10-2016, Published online:
03-12-2016
Corresponding author:
Dwi Liliek
Kusindarta, e-mail: indarta@ugm.ac.id
Citation:
Kusindarta DL, Wihadmadyatami H, Haryanto A (2016) Ocimum
sanctum Linn. stimulate the expression of choline
acetyltransferase on the human cerebral microvascular endothelial
cells, Veterinary World, 9(12): 1348-1354.
Abstract
Aim:
This research
was conducted to identify the expression of choline
acetyltransferase (ChAT) in human cerebral microvascular
endothelial cells (HCMECs) and to clarify the capability of
Ocimum sanctum Linn. ethanolic extract to stimulate the
presence of ChAT in the aging HCMECs.
Materials and
Methods:
In this study, we
perform an in vitro analysis some in the presence of an
ethanolic extract of O. sanctum Linn. as a stimulator for
the ChAT expression. HCMECs are divided become two groups, the
first is in low passage cells as a model of young aged and the
second is in a high passage as a model of aging. Furthermore to
analysis the expression of ChAT without and with extract
treatments, immunocytochemistry and flow cytometry analysis were
performed. In addition, ChAT sandwich enzyme-linked immunosorbent
assay is developed to detect the increasing activity of the ChAT
under normal, and aging HCMECs on the condition treated and
untreated cells.
Results:
In our in
vitro models using HCMECs, we found that ChAT is expressed
throughout intracytoplasmic areas. On the status of aging, the
ethanolic extract from O. sanctum Linn. is capable to
stimulate and restore the expression of ChAT. The increasing of
ChAT expression is in line with the increasing activity of this
enzyme on the aging treated HCMECs.
Conclusions:
Our
observation indicates that HCMECs is one of the noncholinergic
cells which is produced ChAT. The administrated of O. sanctum
Linn. ethanolic extract may stimulate and restore the
expression of ChAT on the deteriorating cells of HCMECs, thus its
may give nerve protection and help the production of
acetylcholine.
Keywords:
choline acetyltransferase, human cerebral microvascular
endothelial cells, Ocimum sanctum Linn.
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