Open Access
Research
(Published
online:
03-02-2016)
1.
Effect of heat shock protein 70
polymorphism on thermotolerance in Tharparkar cattle -
Sandip Bhat, Pushpendra Kumar, Neeraj Kashyap, Bharti Deshmukh,
Mahesh Shivanand Dige, Bharat Bhushan, Anuj Chauhan, Amit Kumar
and Gyanendra Singh
Veterinary World, 9(2): 113-117
doi:
10.14202/vetworld.2016.113-117
Sandip Bhat:
Division of Animal Genetics, Indian Veterinary Research
Institute, Izatnagar, Uttar Pradesh, India; sandipivri@gmail.com
Pushpendra Kumar:
Division of Animal Genetics, Indian Veterinary Research
Institute, Izatnagar, Uttar Pradesh, India; pushpendra64@gmail.com
Neeraj Kashyap:
Division of Animal Genetics, Indian Veterinary Research
Institute, Izatnagar, Uttar Pradesh, India; neeraj.vety@gmail.com
Bharti Deshmukh:
Department of Animal Genetics and Breeding, Govind Ballabh Pant
University of Agriculture and Technology, Pantnagar, Uttarakhand,
India; bharti.vet@gmail.com
Mahesh Shivanand Dige:
Division of Animal Genetics, Indian Veterinary Research
Institute, Izatnagar, Uttar Pradesh, India; maheshdige@gmail.com
Bharat Bhushan:
Division of Animal Genetics, Indian Veterinary Research
Institute, Izatnagar, Uttar Pradesh, India; bbhushan_ivri2003@yahoo.co.in
Anuj Chauhan:
Division of Animal Genetics, Indian Veterinary Research
Institute, Izatnagar, Uttar Pradesh, India; anuj_vet99@rediffmail.com
Amit Kumar:
Division of Animal Genetics, Indian Veterinary Research
Institute, Izatnagar, Uttar Pradesh, India; vetamitchandan07@gmail.com
Gyanendra Singh:
Division of Physiology and Climatology, Indian Veterinary
Research Institute, Izatnagar, Uttar Pradesh, India; gyanendra@ivri.res.in
Received: 17-05-2015, Revised: 05-12-2015, Accepted: 18-12-2015,
Published online: 03-02-2016
Corresponding author:
Sandip Bhat, e-mail: sandipivri@gmail.com
Citation:
Bhat S, Kumar P, Kashyap N, Deshmukh B, Dige MS, Bhushan B,
Chauhan A, Kumar A, Singh G (2016) Effect of heat shock protein
70 polymorphism on thermotolerance in Tharparkar cattle,
Veterinary World 9(2):
113-117.
Abstract
Aim:
Out of various members of heat shock protein (HSP) superfamily
which act a molecular chaperon by binding to the denaturing
protein thus stabilizing them and preserving their activity,
HSP70 are of major importance in thermotolerance development.
Thus, present investigation aimed at a screening of HSP70 gene
for polymorphisms and possible differences in thermotolerance in
Tharparkar breed of cattle.
Materials and Methods:
A 295 bp fragment of HSP70 gene was subjected to polymerase
chain reaction-singlestrand conformation polymorphism (SSCP)
followed by sequencing of different SSCP patterns in 64
Tharparkar cattle. A comparative thermotolerance of identified
genotypes was analyzed using heat tolerance coefficients (HTCs)
of animals for different seasons.
Results:
Three SSCP patterns and consequently two alleles namely A and B
were documented in one fragment of HSP70 gene. On sequencing,
one single-nucleotide polymorphism with G > T substitution was
found at a position that led to a change of amino acid aspartate
to tyrosine in allele A. It was found that in maintaining near
normal average rectal temperature, genotype AA was superior
(p≤0.01). Genotype AA, thus, was found to be most thermotolerant
genotype with the highest HTC (p≤0.01).
Conclusion:
The polymorphism at HSP70 is expected to be a potent determinant
for heat tolerance in cattle, which may aid in selection for
thermotolerance in cattle.
Keywords:
cattle, heat tolerance, heat shock protein 70, polymorphism,
Tharparkar.
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