Open Access
Research
(Published
online:
16-02-2016)
11.
Isolation, antibiogram and pathogenicity of
Salmonella
spp. recovered from slaughtered food
animals in Nagpur region of Central India -
D. G. Kalambhe, N. N. Zade, S. P. Chaudhari, S. V. Shinde, W.
Khan and A. R. Patil
Veterinary World, 9(2): 176-181
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doi:
10.14202/vetworld.2016.176-181
D. G. Kalambhe:
Department of Veterinary Public Health, Nagpur Veterinary
College, Nagpur, Maharashtra, India; drdeepalikalambhe@gmail.com
N. N. Zade:
Department of Veterinary Public Health, Nagpur Veterinary
College, Nagpur, Maharashtra, India; n_zade@rediffmail.com
S. P. Chaudhari:
Department of Veterinary Public Health, Nagpur Veterinary
College, Nagpur, Maharashtra, India; sandeep_vph@gmail.com
S. V. Shinde:
Department of Veterinary Public Health, Nagpur Veterinary
College, Nagpur, Maharashtra, India; shilpi_shri5@rediffmail.com
W. Khan:
Department of Veterinary Public Health, Nagpur Veterinary
College, Nagpur, Maharashtra, India; waqar_khan73@rediffmail.com
A. R. Patil:
Department of Veterinary Public Health, Nagpur Veterinary
College, Nagpur, Maharashtra, India; drarchanavph@gmail.com
Received: 11-08-2015, Revised: 24-12-2015, Accepted: 31-12-2015,
Published online: 16-02-2016
Corresponding author:
D. G. Kalambhe, e-mail: drdeepalikalambhe@gmail.com
Citation:
Kalambhe DG, Zade NN, Chaudhari SP, Shinde SV, Khan W, Patil AR
(2016) Isolation, antibiogram and pathogenicity of
Salmonella
spp. recovered from slaughtered food animals in Nagpur region of
Central India,
Veterinary World 9(2):
176-181.
Abstract
Aim:
To determine the prevalence, antibiogram and pathogenicity of
Salmonella
spp. in the common food animals slaughtered for consumption
purpose at government approved slaughter houses located in and
around Nagpur region during a period of 2010-2012.
Materials and Methods:
A total of 400 samples comprising 50 each of blood and meat from
each slaughtered male cattle, buffaloes, pigs and goats were
collected. Isolation was done by pre-enrichment in buffered
peptone water and enrichment in Rappaport-Vassiliadis broth with
subsequent selective plating onto xylose lysine deoxycholate
agar. Presumptive
Salmonella
colonies were biochemically confirmed and analyzed for
pathogenicity by hemolysin production and Congo red dye binding
assay (CRDA). An antibiotic sensitivity test was performed to
assess the antibiotic resistance pattern of the isolates.
Results:
A total of 10 isolates of
Salmonella
spp. from meat (3 from cattle, 1 from buffaloes and 6 from pigs)
with an overall prevalence of 5% among food animals was
recorded. No isolation was reported from any blood samples.
Pathogenicity assays revealed 100% and 80% positivity for CRDA
and hemolytic activity, respectively. Antimicrobial sensitivity
test showed multi-drug resistance. The overall resistance of 50%
was noted for trimethoprim followed by ampicillin (20%). A
maximum sensitivity (80%) was reported to gentamycin followed by
40% each to ampicillin and trimethoprim, 30% to amikacin and 10%
to kanamycin.
Conclusion:
The presence of multidrug resistant and potentially pathogenic
Salmonella
spp. in slaughtered food animals in Nagpur region can be a
matter of concern for public health.
Keywords:
antibiogram, Congo red binding assay, food animals, hemolysis,
Salmonella
Typhimurium.
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