Open Access
Research
(Published
online: 18-02-2016)
12.
Effect of incubation on
freezability of cholesterol-loaded cyclodextrin treated buffalo
(Bubalus bubalis) spermatozoa -
S. A. Lone,
J. K. Prasad, S. K. Ghosh,
G. K. Das, B. Balamurugan,
R. Katiyar
and M. R. Verma
Veterinary World, 9(2): 182-185
doi:
10.14202/vetworld.2016.182-185
S. A. Lone :
Division of
Animal Reproduction, Gynecology & Obstetrics, National Dairy
Research Institute, Karnal - 132001, Haryana, India;
drloneshabir@gmail.com
J. K. Prasad :
Germplasm Centre,
Division of Animal Reproduction, Indian Veterinary Research
Institute, Izatnagar, Bareilly - 243122, Uttar Pradesh, India;
jkprasad2001@yahoo.com
S. K. Ghosh :
Germplasm Centre,
Division of Animal Reproduction, Indian Veterinary Research
Institute, Izatnagar, Bareilly - 243122, Uttar Pradesh, India;
subratakghosh@yahoo.com
G. K. Das :
Germplasm Centre,
Division of Animal Reproduction, Indian Veterinary Research
Institute, Izatnagar, Bareilly - 243122, Uttar Pradesh, India;
gkdasivri@gmail.com
B. Balamurugan :
Germplasm Centre,
Division of Animal Reproduction, Indian Veterinary Research
Institute, Izatnagar, Bareilly - 243122, Uttar Pradesh, India;
balavet07@gmail.com
R. Katiyar :
Germplasm Centre,
Division of Animal Reproduction, Indian Veterinary Research
Institute, Izatnagar, Bareilly - 243122, Uttar Pradesh, India;
rahul.katiyarvet@gmail.com
M. R. Verma :
Division of
Livestock Economics and Statistics, Indian Veterinary Research
Institute, Izatnagar, Bareilly - 243122, Uttar Pradesh, India;
medramverma@rediffmail.com
Received: 02-09-2015, Revised: 21-12-2015, Accepted: 29-12-2015,
Published online: 18-02-2016
Corresponding author:
S. A. Lone, e-mail: drloneshabir@gmail.com
Citation:
Lone SA, Prasad JK, Ghosh SK, Das GK, Balamurugan B, Katiyar R,
Verma MR (2016) Effect of incubation on freezability of
cholesterol-loaded cyclodextrin treated buffalo (Bubalus
bubalis) spermatozoa, Veterinary World 9(2):
182-185.
Abstract
Aim:
The aim of
this study was to investigate the effect of incubation on
freezability of cholesterol loaded cyclodextrin (CLC) treated
buffalo spermatozoa.
Materials and
Methods:
Semen samples
with mass motility of 3+ and greater, collected from Murrah
buffalo bulls were utilized. Immediately after collection, four
equal groups of semen sample were made. Group I was kept as
control and diluted with Tris upto concentration of 60×106
sperm/ml, where as Groups II, III, and IV were
treated with CLC at 3 mg/120× 106
spermatozoa, incubated at 37°C for action of CLC
for 10, 15 and 20 min, respectively, and diluted with tris upto
concentration of 60×106
sperm/ml. All groups were subjected to
equilibration and freezing. The evaluation of semen samples from
all groups was carried out at fresh, pre-freeze and post-thaw
stage for progressive motility, viability and hypo-osmotic
swelling response (HOS response).
Results:
At
the pre-freeze stage, significantly (p<0.05) higher percentage
of progressive motility and viability was observed in treatment
groups as compared to control with no significant difference
among treatment groups. HOS response was significantly (p<0.05)
higher in treatment groups as compared to control at
pre-freeze stage.
At post-thaw stage,
significantly
(p<0.05) higher percentage of progressive motility, viability
and HOS response was recorded in Group II as compared to control
and other treatment groups (III and IV). Group II retained
significant post-thaw motility and viability at various
post-thaw incubation periods.
Conclusion:
Incubation period of 10 min for CLC treated buffalo spermatozoa
yielded significantly higher results in terms of freezability as
compared to incubation for 15 and 20 min.
Keywords:
buffalo spermatozoa, cholesterol loaded cyclodextrin,
incubation.
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