Open Access
Research
(Published
online: 20-02-2016)
15.
Effect of management system and
season on semen freezability in Jakhrana bucks
-
Narendra Kumar,
B. Rai,
Showkat A. Bhat, S. D. Kharche, Chetna Gangwar,
S. K. Jindal
and Subhash Chandra
Veterinary World, 9(2): 199-202
doi:
10.14202/vetworld.2016.199-202
Narendra Kumar :
Department of
Livestock Production and Management, ICAR - National Dairy
Research Institute, Karnal - 132 001, Haryana, India;
narendrakumarvet@gmail.com
B. Rai :
Department of
Physiology Reproduction and Shelter Management, ICAR- Central
Institute for Research on Goats, Mathura - 281 001, Uttar
Pradesh, India; brai21464@gmail.com
Showkat A. Bhat :
Department of
Livestock Production and Management, ICAR - National Dairy
Research Institute, Karnal - 132 001, Haryana, India;
drshowkatbhat813@gmail.com
S. D. Kharche :
Department of
Physiology Reproduction and Shelter Management, ICAR- Central
Institute for Research on Goats, Mathura - 281 001, Uttar
Pradesh, India; kharche62@gmail.com
Chetna Gangwar :
Department of
Physiology Reproduction and Shelter Management, ICAR- Central
Institute for Research on Goats, Mathura - 281 001, Uttar
Pradesh, India; chetnaom82@gmail.com
S. K. Jindal :
Department of
Physiology Reproduction and Shelter Management, ICAR- Central
Institute for Research on Goats, Mathura - 281 001, Uttar
Pradesh, India; Jindalmathura@gmail.com
Subhash Chandra :
Department of
Livestock Production and Management, ICAR - National Dairy
Research Institute, Karnal - 132 001, Haryana, India;
subhashchandra20july@gmail.com
Received: 27-09-2015, Revised: 30-12-2015, Accepted: 12-01-2016,
Published online: 20-02-2016
Corresponding author:
Narendra Kumar, e-mail: narendrakumarvet@gmail.com
Citation:
Kumar N, Rai B, Bhat SA, Kharche SD, Gangwar C, Jindal SK,
Chandra S (2016) Effect of management system and season on semen
freezability in Jakhrana bucks, Veterinary World 9(2):
199-202.
Abstract
Aim:
The
objective of the study was to determine the effect of the
management system (intensive and semi-intensive) and season
(autumn and winter) on semen freezability in Jakhrana bucks.
Materials and
Methods:
A total of 24
Jakhrana bucks of same body weight and age (BW=30 kg, age=1
year) were randomly allotted into two groups, viz., Group
I (intensive system, 12 bucks) and Group II (semi-intensive
system, 12 bucks). These two groups were statistically tested
for their homogeneity with respect to age and BW. Semen was
collected twice weekly using an artificial vagina during two
seasons: autumn (September-November) and winter
(December-February). A total of 240 semen samples (120 from each
group and season) were evaluated for post-thaw motility (PTM),
viability, abnormality, functional membrane integrity
(hypo-osmotic swelling [HOS]) response and acrosomal integrity.
Results:
The
mean values of PTM and acrosomal integrity of spermatozoa were
significantly (p<0.01) higher in Group II as compared to Group
I. The mean values of viability and abnormality were also
differed significant (p<0.05) between groups. However, the mean
values of HOS response were found non-significant (p>0.05)
between groups. The season showed a significant effect on all
parameters except viability and HOS response. The PTM and
acrosomal integrity of spermatozoa were significantly (p<0.01)
higher in winter as compared to autumn season. Abnormality of
spermatozoa was significantly (p<0.05) lower in winter season.
Conclusions:
This
study indicates that both management system and season influence
semen freezability. The semen collected from bucks reared under
the semi-intensive system and winter season showed better semen
freezability characteristics.
Keywords:
freezability, Jakhrana, management system, season.
References
1. Sharma, M., Singh, M., Kapoor, S. and Jaiswal, S. (2012)
Inter relationship between some routine semen evaluation
parameters in Jersey × local hill cattle crossbred bulls.
Open Vet. J., 2: 26-31.
PMid:26623288 PMCid:PMC4655774 |
|
|
|
2. Kharche, S.D., Jindal, S.K., Priyadharsini, R., Kumar,
S., Goel, A.K., Ramachandran, N. and Rout, P.K. (2013)
Fertility following frozen semen artificial insemination in
Jamnapari goats. Indian J. Anim. Sci., 83: 1071-1073. |
|
|
3. Salamon, S. and Maxwell, W.M. (2000) Storage of ram
semen. Anim. Reprod. Sci., 62: 77-111.
http://dx.doi.org/10.1016/S0378-4320(00)00155-X |
|
|
|
4. Moghaddam, G.H., Pourseif, M.M. and Rafat, S.A. (2012)
Seasonal variation in semen quantity and quality traits of
Iranian crossbred rams. Slovak J. Anim. Sci., 45(3): 67-75. |
|
|
|
5. Guérin, Y., Cognié, Y. and Poulin, N. (1992) Freezability
of freshly ejaculated and frozen ram semen in vitro. In:
Proceedings of the 12th International Congress on Animal
Reproduction. Vol. 3. The Hague, The Netherlands.
p1418-1420. |
|
|
|
6. Campbell, R.C., Hancock, J.L. and Rothschild, L. (1953)
Counting live and dead bulls spermatozoa. J. Exp. Biol., 30:
44-49. |
|
|
|
7. Gangwar, C., Ranjan, R., Kumar, S., Kharche, S.D., Goel,
A.K., Ramachandran, N. and Jindal, S.K. (2014) Use of
chelating agent for optimum post thaw quality of buck semen.
Indian J. Anim. Sci., 84: 8. |
|
|
8. Watson, P.F. (1975) Use of a Giemsa stain to detect
changes in acrosomes of frozen ram spermatozoa. Vet.
Record., 97(1): 12-15.
http://dx.doi.org/10.1136/vr.97.1.12 |
|
|
|
9. Harvey, W.R. (1990) Users Guide for LSMLMW and MIXMDL,
Mixed Model Least Squares and Maximum Likelihood Computer
Program. PC-2 Version, the Ohio State University, Columbus,
USA. |
|
|
10. Kramer, C.Y (1957) Extension of multiple range tests to
group correlated adjusted means. Biometrics, 13: 13-18.
http://dx.doi.org/10.2307/3001898 |
|
|
|
11. Tharasanit, N.A.J. and Techakumphu, N.P.J. (2013)
Glycerol concentration effects on quality and longevity of
post-thaw goat semen. Thai J. Vet. Med., 43(2): 179-186. |
|
|
12. Ranjan, R., Goel, A.K., Ramachandran, N., Kharche, S.D.
and Jindal, S.K. (2015) Effect of egg yolk levels and
equilibration periods on freezability of Jamunapari buck
semen. Indian J. Small Rumin., 21(1): 32-36.
http://dx.doi.org/10.5958/0973-9718.2015.00027.6 |
|
|
|
13. Daskin, A., Kulaksiz, R., Ergun, A. and Halil, E. (2011)
The effect of different dilution rates of Angora buck semen
frozen with Bioxcell® extender on the post-thaw quality. J.
Fac. Vet. Med. Univ. Erciyes, 8(1): 23-26. |
|
|
|
14. Thakur, Y.P., Singh, M. and Jasial, S. (2005) Semen
production and freezability attributes of Chegu Pashmina
bucks. Indian J. Anim. Sci., 75(10): 1165-1167. |
|
|
|
15. Priyadharsini, R., Jindal, S.K., Sharma, D.,
Ramachandran, N., Karche, S.D. and Goel, A.K. (2011) Effect
of different egg yolk level on the cryopreservation
capability of Jakhrana goat semen. J. Anim. Sci. Adv., 1(1):
28-37. |
|
|
16. Aboagla, E.M. and Terada, T. (2004) Effects of egg yolk
during the freezing step of cryopreservation on the
viability of goat spermatozoa. Theriogenology, 62:
1160-1172.
http://dx.doi.org/10.1016/j.theriogenology.2004.01.013
PMid:15289055 |
|
|
17. Cabrera, F., Gonzalez, F., Batista, M., Calero, P.,
Medrano, A. and Gracia, A. (2005) The effect of removal of
seminal plasma, egg yolk level and season on sperm
freezability of canary buck (Capra hircus). Reprod. Domest.
Anim., 40: 191-195.
http://dx.doi.org/10.1111/j.1439-0531.2005.00544.x
PMid:15943690 |
|
|
18. Dorado, J., Rodriguez, I. and Hidalgo, M. (2007)
Cryopreservation of goat spermatozoa: Comparison of two
freezing extenders based on post-thaw sperm quality and
fertility rates after artificial insemination.
Theriogenology, 68: 168-177.
http://dx.doi.org/10.1016/j.theriogenology.2007.04.048
PMid:17532460 |
|
|
19. Bamba, K. and Cran, D.G. (1988) Effect of rapid warming
of bull and rabbit semen. J. Rep. Fertil., 82(2): 501-507.
http://dx.doi.org/10.1530/jrf.0.0820501 |
|
|
|
20. Al-Badry, K.I. (2012) Effect of various thawing times
and temperatures on frozen semen quality of Friesian bulls
in Iraq. Int. J. Anim. Vet. Adv., 4(6): 384-388. |
|
|
21. Watson, P.F. (2000) The causes of reduced fertility with
cryopreserved semen. Anim. Reprod. Sci., 60: 481-492.
http://dx.doi.org/10.1016/S0378-4320(00)00099-3 |
|
|
22. Ahmad, M., Nasrullah, R., Riaz, H., Sattar, A. and
Ahmad, N. (2014) Changes in motility, morphology, plasma
membrane and acrosome integrity during stages of
cryopreservation of buck sperm. J. S. Afr. Vet. Assoc.,
85(1): 01-04.
http://dx.doi.org/10.4102/jsava.v85i1.972 |
|
|
|
23. Apu, A.S., Khandoker, M.Y., Husain, S.S., Fakruzzaman,
M. and Notter, D.R. (2012) Comparative study of fresh and
frozen-thawed quality in relation to fertility of black
Bengal goats. Iran. J. Appl. Anim. Sci., 2(2): 157-161. |
|
|
24. Murase, T., Imaeda, N., Yamada, H. and Miyazawa, K.
(2007) Seasonal changes in semen characteristics,
composition of seminal plasma and frequency of acrosome
reaction induced by calcium and calcium ionophore A23187 in
Large White boars. J. Reprod. Dev., 53(4): 853-865.
http://dx.doi.org/10.1262/jrd.19026 |
|
|
25. Barranco, I., Ortega, M.D., Martinez-Alborcia, M.J.,
Vazquez, J.M., Martinez, E.A. and Roca, J. (2013) Season of
ejaculate collection influences the freezability of boar
spermatozoa. Cryobiology, 67(3): 299-304.
http://dx.doi.org/10.1016/j.cryobiol.2013.09.001
PMid:24045067 |
|
|
26. Tuli, R.K. and Holtz, W. (1995) Effect of season on the
freezability of Boer goat semen in Northern Temperate zone.
Theriogenology, 43: 1359-1363.
http://dx.doi.org/10.1016/0093-691X(95)00120-W |
|
|
27. D'alessandro, A.G. and Martemucci, G. (2003) Evaluation
of seasonal variations of semen freezability in Leccese ram.
Anim. Reprod. Sci., 79(1): 93-102.
http://dx.doi.org/10.1016/S0378-4320(03)00113-1 |
|
|
28. Wang, W., Luo, J., Sun, S., Xi, L., Gao, Q., Haile, A.B.
and Zhang, W. (2015) The effect of season on spermatozoa
motility, plasma membrane and acrosome integrity in fresh
and frozen-thawed semen from Xinong Saanen bucks. Reprod.
Domest. Anim., 50(1): 23-28.
http://dx.doi.org/10.1111/rda.12444
PMid:25366190 |
|
