Open Access
Research
(Published
online: 27-02-2016)
18.
Comparison of different diagnostic
techniques for the detection of cryptosporidiosis in bovines -
H. K. M. Rekha, G. C. Puttalakshmamma and Placid E. D’Souza
Veterinary World, 9(2): 211-215
doi:
10.14202/vetworld.2016.211-215
H. K. M. Rekha:
Department of Animal Husbandary & Veterinary Services,
Government of Karnataka, Muthur, Piriapatna, Mysore, Karnataka,
India; rekhahkm565@gmail.com
G. C. Puttalakshmamma:
Department of Parasitology, Veterinary College, Hebbal,
Bengaluru, Karnataka, India; puttalakshmamma@gmail.com
Placid E. D’Souza:
Department of Veterinary Parasitology, Veterinary College,
Hassan, Karnataka, India; placid536@gmail.com
Received: 12-08-2015, Revised: 28-12-2015, Accepted: 08-01-2016,
Published online: 27-02-2016
Corresponding author:
H. K. M. Rekha, e-mail: rekhahkm565@gmail.com
Citation:
Rekha HKM, Puttalakshmamma GC, D’Souza PE (2016) Comparison of
different diagnostic techniques for the detection of
cryptosporidiosis in bovines,
Veterinary World,
9(2):
211-215.
Abstract
Aim:
Aim of the present study was to compare different methods,
viz.,
Sheather’s sugar flotation (SSF), Ziehl-Neelsen (ZN), Kinyoun’s
acid-fast method (KAF), safranin-methylene blue staining (SMB),
and negative staining techniques such as nigrosin staining,
light green staining, and malachite green staining for the
detection of
Cryptosporidium
spp. oocysts in bovines.
Materials and Methods:
A total of 455 fecal samples from bovines were collected from
private, government farms and from the clinical cases presented
to Department of Medicine, Veterinary College, Bengaluru. They
were subjected for SSF, ZN, KAF, SMB and
negative staining methods.
Results:
Out of 455 animal fecal samples screened 5.71% were found
positive for
Cryptosporidium
spp. oocysts. The species were identified as
Cryptosporidium parvum
in calves and
Cryptosporidium andersoni
in adults based on the morphological characterization and
micrometry of the oocysts.
Conclusions:
Of all the techniques, fecal flotation with sheather’s was found
to be more specific and sensitive method for the detection of
Cryptosporidium
spp. oocysts. Among the conventional staining methods, the SMB
gives better differentiation between oocysts and yeast. Among
the three negative staining methods, malachite green was found
sensitive over the other methods.
Keywords:
bovines,
Cryptosporidium,
Kinyoun’s acid-fast method, modified Ziehl–Neelsen, oocysts,
safranin-methylene blue staining, SSF, diagnostic methods.
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