Open Access
Research
(Published
online:
12-02-2016)
6.
Loktanella
spp. Gb03 as an algicidal bacterium, isolated from the culture
of
Dinoflagellate Gambierdiscus belizeanus -
Anmar Hameed Bloh, Gires Usup and Asmat Ahmad
Veterinary World, 9(2): 142-146
doi:
10.14202/vetworld.2016.142-146
Anmar Hameed Bloh:
School of Bioscience and Biotechnology, Faculty of Science and
Technology, University Kebangsaan Malaysia, 43600 Bangi,
Selangor, Malaysia; anmarhameed@yahoo.com
Gires Usup:
School of Environmental and Natural Resources Sciences, Faculty
of Science and Technology, University Kebangsaan Malaysia, 43600
Bangi, Selangor, Malaysia; gires@ukm.my
Asmat Ahmad:
School of Bioscience and Biotechnology, Faculty of Science and
Technology, University Kebangsaan Malaysia, 43600 Bangi,
Selangor, Malaysia; asmat@ukm.edu.my
Received: 01-10-2015, Revised: 23-12-2015, Accepted: 29-12-2015,
Published online: 12-02-2016
Corresponding author:
Asmat Ahmad, e-mail: asmat@ukm.edu.my
Citation:
Bloh AH, Usup G, Ahmad A (2016)
Loktanella
spp. Gb03 as an algicidal bacterium, isolated from the culture
of
Dinoflagellate Gambierdiscus belizeanus, Veterinary World 9(2):
142-146.
Abstract
Aim:
Bacteria associated with harmful algal blooms can play a crucial
role in regulating algal blooms in the environment. This study
aimed at isolating and identifying algicidal bacteria in
Dinoflagellate
culture and to determine the optimum growth requirement of the
algicidal bacteria,
Loktanella
sp. Gb-03.
Materials and Methods:
The
Dinoflagellate
culture used in this study was supplied by Professor Gires
Usup’s Laboratory, School of Environmental and Natural Resources
Sciences, Faculty of Science and Technology, University
Kebangsaan Malaysia, Malaysia. The culture was used for the
isolation of
Loktanella
sp., using biochemical tests, API 20 ONE kits. The fatty acid
content of the isolates and the algicidal activity were further
evaluated, and the phenotype was determined through the
phylogenetic tree.
Results:
Gram-negative, non-motile, non-spore-forming, short rod-shaped,
aerobic bacteria (Gb01, Gb02, Gb03, Gb04, Gb05, and Gb06) were
isolated from the
Dinoflagellate
culture. The colonies were pink in color, convex with a smooth
surface and entire edge. The optimum growth temperature for the
Loktanella
sp. Gb03 isolate was determined to be 30°C, in 1% of NaCl and
pH7. Phylogenetic analysis based on 16S rRNA gene sequences
showed that the bacterium belonged to the genus
Loktanella
of the class Alphaproteobacteria and formed a tight cluster with
the type strain of
Loktanella pyoseonensis
(97.0% sequence similarity).
Conclusion:
On the basis of phenotypic, phylogenetic data and genetic
distinctiveness, strain Gb-03, were placed in the genus
Loktanella
as the type strain of species. Moreover, it has algicidal
activity against seven toxic
Dinoflagellate.
The algicidal property of the isolated
Loktanella
is vital, especially where biological control is needed to
mitigate algal bloom or targeted
Dinoflagellates.
Keywords:
algicidal activity,
Gambierdiscus belizeanus,
Loktanella
sp. Gb-03, optimization growth.
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