Open Access
R esearch
(Published
online:
21-01-2016)
13.
Prevalence of vero toxic
Escherichia coli
in fecal samples of domestic as well as
wild ruminants in Mathura districts and Kanpur zoo -
Raghavendra Prasad Mishra, Udit Jain, Basanti Bist, Amit Kumar
Verma and Ashok Kumar
Veterinary World, 9(1): 71-74
doi:
10.14202/vetworld.2016.71-74
Raghavendra Prasad Mishra:
Department of Veterinary Public Health, College of Veterinary
Sciences and Animal Husbandry, Pandit Deen Dayal Upadhayay Pashu
Chikitsa Vigyan Vishvidhyalaya Ewam Go-Anusandhan Sansthan,
Mathura - 281 001, Uttar Pradesh, India; rmishra523@rediffmail.com
Udit Jain:
Department of Veterinary Public Health, College of Veterinary
Sciences and Animal Husbandry, Pandit Deen Dayal Upadhayay Pashu
Chikitsa Vigyan Vishvidhyalaya Ewam Go-Anusandhan Sansthan,
Mathura - 281 001, Uttar Pradesh, India; druditjain@hotmail.com
Basanti Bist:
Department of Veterinary Public Health, College of Veterinary
Sciences and Animal Husbandry, Pandit Deen Dayal Upadhayay Pashu
Chikitsa Vigyan Vishvidhyalaya Ewam Go-Anusandhan Sansthan,
Mathura - 281 001, Uttar Pradesh, India; vasanti.bist@gmail.com
Amit Kumar Verma:
Department of Veterinary Epidemiology and Preventive Medicine,
College of Veterinary Sciences and Animal Husbandry, Pandit Deen
Dayal Upadhayay Pashu Chikitsa Vigyan Vishvidhyalaya Ewam Go-Anusandhan
Sansthan, Mathura - 281 001, Uttar Pradesh, India; drakverma79@gmail.com
Ashok Kumar:
Division of Veterinary Public Health, Indian Veterinary Research
Institute, Izatnagar, Bareilly, Uttar Pradesh, India; ashokakt@rediffmail.com
Received: 28-08-2015, Revised: 08-12-2015, Accepted: 14-12-2015,
Published online: 21-01-2016
Corresponding author:
Raghavendra Prasad Mishra, e-mail: rmishra523@rediffmail.com
Citation:
Mishra RP, Jain U, Bist B, Verma AK, Kumar A (2016) Prevalence
of vero toxic
Escherichia coli
in fecal samples of domestic as well as wild ruminants in
Mathura districts and Kanpur zoo,
Veterinary World 9(1):
71-74.
Abstract
Aim:
The present study was planned to reveal the prevalence of
verocytotoxigenic
Escherichia coli
(VTEC) in fecal samples of domestic and wild ruminants in
Mathura district and Kanpur zoo.
Materials and Methods:
A total of 240 fecal samples comprising 60 each of cattle,
buffalo, sheep and deer from Mathura districts and Kanpur zoo
were screened for the presence of
E. coli
and VTEC genes positive by polymerase chain reaction (PCR).
Result:
Out of 240 fecal samples, 212
E. coli
strains were obtained. All the
E. coli
isolates were screened by PCR to detect virulence genes
stx1, stx2, eaeA
and
hlyA.
Of these, 25 isolates were identified as VTEC. The prevalence of
VTEC in cattle, buffalo, sheep and deer was found 13.4% (8/60),
13.4% (8/60), 6.67% (4/60) and 8.33% (5/60), respectively.
Conclusion:
stx1, stx2, eaeA
and
hlyA
genes were prevalent in VTEC isolates from feces of cattle,
buffalo, sheep and deer population of Mathura districts and
Kanpur zoo. The presence of VTEC isolates in this region may
pose a threat to public health.
Keywords:
domestic and wild ruminants, feces, prevalence, polymerase chain
reaction, verotoxic
Escherichia coli.
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