Open Access
Research
(Published
online: 16-07-2016)
10.
Immuno-pathological studies on broiler
chicken experimentally infected with Escherichia coli and
supplemented with neem (Azadirachta indica) leaf extract
-
Vikash Sharma, K. K. Jakhar
and Swati Dahiya
Veterinary World, 9(7): 735-741
doi:
10.14202/vetworld.2016.735-741
Vikash Sharma :
Department of
Veterinary Pathology, Lala Lajpat Rai University of Veterinary &
Animal Sciences, Hisar, Haryana, India;
sharmavikashjind@gmail.com
K. K. Jakhar :
Department of
Veterinary Pathology, Lala Lajpat Rai University of Veterinary &
Animal Sciences, Hisar, Haryana, India;
hod.vpp@llruvas.edu.in
Swati Dahiya :
Department of
Veterinary Microbiology, Lala Lajpat Rai University of
Veterinary & Animal Sciences, Hisar, Haryana, India; swatidahiya@luvas.edu.in
Received: 08-04-2016, Accepted: 15-06-2016, Published online:
16-07-2016
Corresponding author:
Vikash
Sharma, e-mail: sharmavikashjind@gmail.com
Citation:
Sharma V, Jakhar KK, Dahiya S (2016) Immuno-pathological studies
on broiler chicken experimentally infected with Escherichia
coli and supplemented with neem (Azadirachta indica)
leaf extract, Veterinary World, 9(7): 735-741.
Abstract
Aim:
The present
study was conducted to evaluate the effects of neem leaf extract
(NLE) supplementation on immunological response and pathology of
different lymphoid organs in experimentally Escherichia coli
challenged broiler chickens.
Materials and
Methods:
For this study,
we procured 192-day-old broiler chicks from local hatchery and
divided them into Groups A and Group B containing 96 birds each
on the first day. Chicks of Group A were supplemented with 10%
NLE in water, whereas chicks of Group B were not supplemented
with NLE throughout the experiment. At 7th
day of age, chicks of Group A were divided into A1
and A2 and Group B into B1 and B2 with 54 and 42 chicks,
respectively, and chicks of Groups A1 and B1 were injected with
E. coli O78 at 107
colony-forming units/0.5 ml intraperitoneally. Six
chicks from each group were sacrificed at 0, 2, 4, 7, 14, 21,
and 28 days post infection; blood was collected and thorough
post-mortem examination was conducted. Tissue pieces of spleen
and bursa of Fabricius were collected in 10% buffered formalin
for histopathological examination. Serum was separated for
immunological studies.
Result:
E.
coli
specific antibody titer was significantly higher in Group A1 in
comparison to Group B1. Delayed-type hypersensitivity response
against 2,4 dinirochlorobenzene (DNCB) antigen was significantly
higher in Group A1 as compared to Group B1. Pathological studies
revealed that E. coli infection caused depletion of
lymphocytes in bursa of Fabricius and spleen. Severity of
lesions in Group A1 was significantly lower in comparison to
Group B1.
Conclusion:
10%
NLE supplementation enhanced the humoral as well as cellular
immune responses attributed to its immunomodulatory property in
experimentally E. coli infected broiler chicken.
Keywords:
broiler chicken, Escherichia coli, immune response, neem
leaf extract.
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