Open Access
Research
(Published
online: 12-07-2016)
7.
A comparison of titers of anti-Brucella
antibodies of naturally infected and healthy vaccinated
cattle by standard tube agglutination test, microtiter plate
agglutination test, indirect hemagglutination assay, and
indirect enzyme-linked immunosorbent assay -
Anju Mohan, Hari Mohan Saxena and Puneet Malhotra
Veterinary World, 9(7): 717-722
doi:
10.14202/vetworld.2016.717-722
Anju Mohan:
Department of Veterinary Microbiology, College of Veterinary
Science, Guru Angad Dev Veterinary and Animal Sciences
University, Ludhiana - 141 004, Punjab, India; anz089@gmail.com
Hari Mohan Saxena:
Department of Veterinary Microbiology, College of Veterinary
Science, Guru Angad Dev Veterinary and Animal Sciences
University, Ludhiana - 141 004, Punjab, India; hmsaxena@yahoo.com
Puneet Malhotra:
Department of Animal Genetics and Breeding, College of
Veterinary Science, Guru Angad Dev Veterinary and Animal
Sciences University, Ludhiana - 141 004, Punjab, India;
dr.puneetmalhotra@rediffmail.com
Received: 07-04-2016, Accepted: 08-06-2016, Published online:
12-07-2016
Corresponding author:
Hari Mohan Saxena, e-mail: hmsaxena@yahoo.com
Citation:
Mohan A, Saxena HM, Malhotra P (2016) A comparison of titers of
anti-Brucella antibodies of naturally infected and
healthy vaccinated cattle by standard tube agglutination test,
microtiter plate agglutination test, indirect hemagglutination
assay, and indirect enzyme-linked immunosorbent assay,
Veterinary World, 9(7): 717-722.
Abstract
Aim:
We determined the antibody response in cattle naturally infected
with brucellosis and normal healthy adult cattle vaccinated
during calf hood with strain 19.
Materials and Methods:
The antibody titers were measured by standard tube agglutination
test (STAT), microtiter plate agglutination test (MAT), indirect
hemagglutination assay (IHA), and indirect enzyme-linked
immunosorbent assay (iELISA) as per standard protocols.
Results:
The mean STAT titers were 1.963±0.345 in infected cattle and
1.200±0.155 in healthy vaccinated cattle. The difference was
extremely significant (p<0.0001). The mean MAT titers were
2.244±0.727 in infected cattle and 1.200±0.155 in healthy
vaccinated cattle. The difference was very significant
(p<0.005). The mean IHA titers in infected cattle were
2.284±0.574, and those in healthy vaccinated cattle were
1.200±0.155. The difference was extremely significant
(p=0.0002). However, the difference in mean iELISA titers of
infected cattle (1.3678±0.014) and healthy vaccinated cattle
(1.367±0.014) was non-significant. The infected animals showed
very high titers of agglutinating antibodies compared to the
vaccinated animals. However, it cannot be ascertained whether
these antibodies are due to vaccine or response to infection.
Since the infected animals had been vaccinated earlier, the
current infection may suggest that vaccination was unable to
induce protective levels of antibody. The heightened antibody
response after infection may also indicate a secondary immune
response to the antigens common to the vaccine strain and wild
Brucella
organisms.
Conclusion:
The brucellosis infected animals showed very high titers of
agglutinating antibodies compared to the vaccinated animals.
Keywords:
antibody titers,
Brucella,
brucellosis,
Brucella abortus
S19 vaccine, bovine brucellosis.
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