Open Access
Research
(Published
online: 20-06-2016)
14.
Effect of acute exposure to nonylphenol on biochemical,
hormonal, and hematological parameters and muscle tissues
residues of Nile tilapia;
Oreochromis niloticus -
Hager Tarek H. Ismail and Heba Hassan H. Mahboub
Veterinary World, 9(6): 616-625
doi:
10.14202/vetworld.2016.616-625
Hager Tarek H. Ismail:
Department of Clinical Pathology, Faculty of Veterinary
Medicine, Zagazig University, Zagazig City, Sharkia Province,
Egypt; hager_vet@hotmail.com / hagar_vet@zu.edu.eg
Heba Hassan H. Mahboub:
Department of Fish Diseases and Management, Faculty of
Veterinary Medicine, Zagazig University, Zagazig City, Sharkia
Province, Egypt; hhhmb@yahoo.com
Received: 07-02-2016, Accepted: 16-05-2016, Published online:
20-06-2016
Corresponding author:
Hager Tarek H. Ismail, e-mail:
hager_vet@hotmail.com / hagar_vet@zu.edu.eg
Citation:
Ismail HTH, Mahboub HHH (2016) Effect of acute exposure to
nonylphenol on biochemical, hormonal, and hematological
parameters and muscle tissues residues of Nile tilapia;
Oreochromis niloticus, Veterinary World, 9(6):
616-625.
Abstract
Aim:
This study is aimed to evaluate some biochemical, hormonal,
hematological, and histopathological changes in Nile tilapia,
Oreochromis niloticus,
after acute exposure to nonylphenol (NP). In addition to
detection of NP residues in the fish, muscle tissues for human
health concern.
Materials and Methods:
A total of 90 apparently healthy Nile tilapia,
O. niloticus,
were randomly divided into three equal groups; each containing
30 fish (three replicates). Groups 1 and 2 kept as a control and
solvent control (acetone), respectively, and Group 3 exposed to
NP at a dose level of 500 μg/L water for 7 successive days.
Blood and tissue samples were collected 2 times randomly from
each group after 7 days from fish exposure to NP and 10 days
from exposure stopping.
Results:
Fish exposed to NP Group 3 showed anorexia, sluggish movement,
erythema of the skin, areas of scales loss, and hemorrhagic
ulcers in some areas of body region leading to exposing the
viscera. Biochemical results revealed a significant increase in
serum total proteins and globulins levels, a highly significant
increase in serum alanine aminotransferase and aspartate
aminotransferase activities, triglycerides, cholesterol, and
creatinine levels, insignificant increase in serum uric acid
level, and a highly significant decrease in serum testosterone
and estradiol-β17 levels in Group 3 in compare with the control
group. Histopathological finding confirms these results. While
hematological results of the same group revealed a significant
increase in red blood cells count and packed cell volume value,
insignificant increase in hemoglobin concentration, leukopenia,
lymphopenia, and monocytopenia in compared with the control
group. All of these changes appeared after 7 days from fish
exposure to NP. Most of these alterations returned toward the
normal level after 10 days from stopping exposure to NP. NP
residues detected in fish muscle tissues of Group 3 during
exposure and after stopping exposure to it.
Conclusion:
It is concluded that NP is a toxic pollutant and has an adverse
effect on fish health and reproduction as well as accumulates in
fish muscle tissues which may cause human health hazard.
Keywords:
biochemical, hematological, hormonal, Nile tilapia, nonylphenol.
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