Open Access
Research
(Published
online: 28-03-2016)
18.
Ultrastructural changes in the sublingual salivary gland of
prenatal buffalo (Bubalus
bubalis)
-
A. D. Singh and Opinder Singh
Veterinary World, 9(3): 326-329
doi:
10.14202/vetworld.2016.326-329
A. D. Singh:
Department of Veterinary Anatomy, Guru Angad Dev Veterinary and
Animal Sciences University, Ludhiana - 141 004, Punjab, India;
dramandeep287@gmail.com
Opinder Singh:
Department of Veterinary Anatomy, Guru Angad Dev Veterinary and
Animal Sciences University, Ludhiana - 141 004, Punjab, India;
singhopinder68@gmail.com
Received: 26-11-2015, Revised: 14-02-2016, Accepted: 20-02-2016,
Published online: 28-03-2016
Corresponding author:
Opinder Singh, e-mail: singhopinder68@gmail.com
Citation:
Singh AD, Singh O (2016) Ultrastructural changes in the
sublingual salivary gland of prenatal buffalo (Bubalus
bubalis),
Veterinary World, 9(3):
326-329.
Abstract
Aim:
The present study was aimed to elucidate ultrastructural changes
in the development of sublingual salivary gland of buffalo
during prenatal life.
Materials and Methods:
The study was carried out on sublingual salivary gland of 36
buffalo fetuses ranging from 13.2 cm curved crown-rump length (CVRL)
(88th day) to full term. The fetuses were categorized into three
groups based on their CVRL.
Results:
The cells lining the terminal tubules were undifferentiated with
poorly developed cytoplasmic organelles but lacked secretory
granules (SGs) at 13.2 cm CVRL (88th day). The SGs appeared
first in the form of membrane-bound secretory vesicles with
homogeneous electron-dense as well as electron-lucent contents
at 21.2 cm CVRL (122nd day); however, mucous acinar cells
contained electron-lucent granules, while serous secretory cells
as well as serous demilunes showed electron-dense granules at 34
cm CVRL (150th day) of prenatal life. At 53.5 cm CVRL (194th
day), both mucous and serous acini were differentiated by the
density of SGs.
Conclusion:
The cytoplasm of acinar cells was filled with mitochondria,
rough endoplasmic reticulum, and Golgi profiles in mid and late
fetal age groups. The SGs were increased in number during the
late fetal age group. The myoepithelial cells (MECs) were
located at the base of the acinar cells as well as intercalated
and striated ducts and were stellate in shape. The
ultrastructure of MEC revealed a parallel stream of myofilaments
in the cytoplasm and its processes. The mucous cells were
predominantly present in the sublingual salivary gland and were
pyramidal in shape.
Keywords:
buffalo, prenatal, sublingual salivary gland, transmission
electron microscopy.
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