Open Access
Research
(Published
online: 02-05-2016)
1.
Minimization of apoptosis-like changes in
cryopreserved buffalo bull sperm by supplementing extender with
Bcl-2 protein -
Jasmer Dalal, Ajeet Kumar, Mrigank Honparkhe, Dipak Deka and
Narinder Singh
Veterinary World, 9(5): 432-436
doi:
10.14202/vetworld.2016.432-436
Jasmer Dalal:
Department of Veterinary Gynaecology and Obstetrics, Guru Angad
Dev Veterinary and Animal Sciences University, Ludhiana, Punjab,
India; jasmer.sl.dalal@gmail.com
Ajeet Kumar:
Department of Veterinary Gynaecology and Obstetrics, Guru Angad
Dev Veterinary and Animal Sciences University, Ludhiana, Punjab,
India; ajeetvet@yahoo.com
Mrigank Honparkhe:
Department of Veterinary Gynaecology and Obstetrics, Guru Angad
Dev Veterinary and Animal Sciences University, Ludhiana, Punjab,
India; honparkhem@rediffmail.com
Dipak Deka:
School of Animal Biotechnology, Guru Angad Dev Veterinary and
Animal Sciences University, Ludhiana, Punjab, India; dekadipak@rediffmail.com
Narinder Singh:
Department of Veterinary Gynaecology and Obstetrics, Guru Angad
Dev Veterinary and Animal Sciences University, Ludhiana, Punjab,
India; narinder2002us@yahoo.com
Received: 28-01-2016, Accepted: 16-04-2016, Published online:
02-05-2016
Corresponding author:
Ajeet Kumar, e-mail: ajeetvet@yahoo.com
Citation:
Dalal J, Kumar A, Honparkhe M, Deka D, Singh N (2016)
Minimization of apoptosis-like changes in cryopreserved buffalo
bull sperm by supplementing extender with Bcl-2 protein,
Veterinary World, 9(5): 432-436.
Abstract
Aim:
This study was aimed at evaluating the anti-apoptotic effects of
Bcl-2 protein in cryopreserved buffalo bull sperm.
Materials and Methods:
A total 10 ejaculates from two buffalo bulls (5 each) were
collected using artificial vagina method, and semen was
evaluated using a standard protocol. Semen was extended by Tris
egg yolk extender supplemented with Bcl-2 protein at 5, 10, and
15 μM. Semen was cryopreserved at ultra-low temperature using
traditional vapor freezing method. Prefreeze and post-thaw semen
samples were evaluated for percent motility, viability,
hypo-osmotic swelling test (HOST) reactive sperms; status of
mitochondrial membrane activity and status of sperm
phospholipase A1 and phospholipase A2 activity.
Results:
There were no significant effects of Bcl-2 protein
supplementation on pre-freeze sperm quality. Percent motility
and active mitochondria in post-thaw Bcl-2 supplemented and
control groups were also similar. However, viable sperms were
significantly (p<0.05) higher (74.29±4.23%) in Bcl-2
supplemented group (5 μM) as compared to control (51.6±5.77%).
The proportion of HOST reactive sperms was also higher
(63.1±6.73%) in Bcl-2 supplemented (5 μM) group as compared to
control (50.7±6.98%). The sperm with low PLA activity
(non-apoptotic) was significantly (p<0.05) higher in all the
supplemented doses of Bcl-2 protein, i.e., at 5 μM
(73.42±5.79%), 10 μM (75.51±6.22%), and 15 μM (74.78±5.89%) as
compared to control (60.23±4.45%). We found that Bcl-2 protein
supplementation at 5 μM dose improved the postthaw semen quality
indicated by higher viability, HOST reactive sperms, and sperm
with low PLA activity (non-apoptotic sperms).
Conclusion:
Bcl-2 protein supplementation exerts its protective effect on
spermatozoa against apoptosis-like changes developed during
cryopreservation.
Keywords:
anti-apoptotic effects, Bcl-2 protein, buffalo bull, semen
cryopreservation.
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