Open Access
Research
(Published
online: 17-11-2016)
17.
Benefits of pomegranate (Punica
granatum
Linn) fruit extracts to weight changes, total protein, and uric
acid in white rats (Rattus
norvegicus)
as an animal model of acute renal failure -
Hardany Primarizky, Wiwik Misaco Yuniarti and Bambang Sektiari
Lukiswanto
Veterinary World, 9(11): 1269-1274
doi:
10.14202/vetworld.2016.1269-1274
Hardany Primarizky:
Department of Veterinary Clinic, Faculty of Veterinary Medicine,
Universitas Airlangga, Surabaya, East Java, Indonesia;
kikken.zeyra@gmail.com
Wiwik Misaco Yuniarti:
Department of Veterinary Clinic, Faculty of Veterinary Medicine,
Universitas Airlangga, Surabaya, East Java, Indonesia; wiwikmisaco@yahoo.com
Bambang Sektiari Lukiswanto:
Department of Veterinary Clinic, Faculty of Veterinary Medicine,
Universitas Airlangga, Surabaya, East Java, Indonesia; bamsekti@yahoo.com
Received: 21-03-2016, Accepted: 13-10-2016, Published online:
17-11-2016
Corresponding author:
Hardany Primarizky, e-mail: kikken.zeyra@gmail.com
Citation:
Primarizky H, Yuniarti WM, Lukiswanto BS (2016) Benefits of
pomegranate (Punica
granatum
Linn) fruit extracts to weight changes, total protein, and uric
acid in white rats (Rattus
norvegicus)
as an animal model of acute renal failure,
Veterinary World, 9(11):
1269-1274.
Abstract
Aim:
The occurrence of acute renal failure (ARF) cases continues to
increase every year. Some of the cases are due to nephrotoxic
effect caused by overdose of antibiotic consumption or abuse of
the drug, gentamicin. An antibiotic therapy that can be used to
overcome in such a case is the pomegranate extracts. However,
until now, studies using pomegranate for cases of ARF have not
been done. This study aims to determine changes in body weight,
the levels of total protein (TP), and the levels of serum uric
acid (UA) as a result of the pomegranate extract consumption.
Materials and Methods:
A
total number of 32 rats (Rattus
norvegicus)
were divided into four groups randomly. One group was assigned as
the control group (P0) and given intraperitoneal (i.p.) saline and
0.3% carboxy methyl cellulose sodium (CMC) Na; P1 was provided
with 80 mg/kg bw/i.p. gentamicin and 0.3% CMC Na orally, P2 was
supplied with 80 mg/kg bw/i.p. gentamicin and ellagic acid in 0.3%
CMC Na, and P3 was given 80 mg/kg bw/i.p. gentamicin and 150 mg/kg
bw pomegranate extract in 0.3% CMC Na. The provision of treatment
was carried out in 8 days and followed by making the overthrow of
body weight and blood sampling for the examination of study
variables.
Results:
The results taken by doing the analysis of variance method for the
four treatment groups show that the control group (P0) has
significant differences from P1, P2, and P3 (p<0.05), but there
are no significant differences among the other three treatment
groups. Meanwhile, the average values of serum UA levels among P1,
P2, and P3 indicate significant differences.
Conclusion:
In conclusion, the administration of pomegranate extracts in the
treatment of nephrotoxicity toward rats is effective to maintain
normal body weight, normal TP levels, and the UA blood serum of
the rats. As this study is a preventive therapy, it needs further
researches about the effective dose as a curative therapy, its
level of effectiveness and its long-term side effects.
Keywords:
acute renal failure, gout, pomegranate extract, total protein.
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