Open Access
Research
(Published
online: 27-11-2016)
23.
Prophylactic effect of Nigella
sativa against lead acetate induced changes in spermiogram,
reproductive hormones and gonadal histology of rats -
Mohammed Abdulrazzaq Assi,
Mohammed Noor Mohd Hezmee,
Yusuf Abba, Md Sabri Md Yusof, Abd Wahid Haron, Mohamed Ali Rajion
and Mashaan Abbas Al-Zuhairy
Veterinary World, 9(11): 1305-1311
doi:
10.14202/vetworld.2016.1305-1311
Mohammed
Abdulrazzaq Assi :
Department of
Community Health, College of Health and Medical Techniques, Al-Furat
Al-Awsat Technical University, Iraq;
Department of
Veterinary Preclinical Sciences, Faculty of Veterinary Medicine,
Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia;
razaq_assi@yahoo.com
Mohammed Noor Mohd
Hezmee :
Department of
Veterinary Preclinical Sciences, Faculty of Veterinary Medicine,
Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia;
hezmee@upm.edu.my
Yusuf Abba :
Department of Veterinary Pathology and Microbiology, Faculty of
Veterinary Medicine, Universiti Putra Malaysia, 43400 Serdang,
Selangor, Malaysia;
yabbavet@gmail.com
Md Sabri Md Yusof :
Department of
Veterinary Pathology and Microbiology, Faculty of Veterinary
Medicine, Universiti Putra Malaysia, 43400 Serdang, Selangor,
Malaysia;
mdsabri@upm.edu.my
Abd Wahid Haron :
Department of
Veterinary Clinical Studies, Faculty of Veterinary Medicine,
Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia;
wahidh@upm.edu.my
Mohamed Ali Rajion :
Department of
Veterinary Preclinical Sciences, Faculty of Veterinary Medicine,
Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia;
marajion@gmail.com
Mashaan Abbas Al-Zuhairy :
Department of
Veterinary Public Health, College of Veterinary Medicine, Baghdad
University, Iraq; mashaanaz@yahoo.com
Received: 08-07-2016, Accepted: 24-10-2016, Published online:
27-11-2016
Corresponding author:
Mohammed Noor
Mohd Hezmee, e-mail: hezmee@upm.edu.my
Citation:
Assi MA, Hezmee MNM, Abba Y, Md. Yusoff MS, Haron AW, Rajion MA,
Al-Zuhairy MA (2016) Prophylactic effect of Nigella sativa
against lead acetate induced changes in spermiogram, reproductive
hormones and gonadal histology of rats, Veterinary World, 9(11):
1305-1311.
Abstract
Aim:
This study was
designed to evaluate the prophylactic effect of Nigella sativa
(NS) treatment on toxic effects induced by lead acetate (LA)
on the reproductive hormones, spermiogram and gonadal histology of
rats.
Materials and
Methods:
A total of 20
Sprague-Dawley rats were divided into four groups of five rats
each. Group 1 (negative control [NC]) was the NC and was given
distilled water, Group 2 served as the positive control (PC) and
was administered 10 mg/kg/day of LA per overall survival (OS),
Group 3 (T1) was administered 200 mg/kg/daily of NS per OS for a
month, and Group 4 (T2) was pretreated with 200 mg/kg/daily of NS
per OS for 1 month, followed by 10 mg/kg/daily of LA alone per OS
for another. The rats were euthanized at the end of the
experimental period for collection of blood and the right caudal
epididymis and testis. Serum was used for determination of
reproductive hormones by using radioimmunoassay kits. The
epididymal segment was cut and homogenized in phosphate-buffered
saline, and the homogenate was used for determination of the
spermiogram parameters such as sperm concentration, sperm
viability, percentage of live sperm, motility and abnormality.
Both the epididymis and testis were fixed in 10% buffered formalin
for histological processing.
Results:
The sperm
concentration, general, and individual motilities were higher
(p<0.05) in the NC and T1 animals, while the T2 had intermediate
and the PC had lower (p<0.05) values of each parameter. The
percentage sperm viability was higher (p<0.05) in the T1 and lower
(p<0.05) in the PC group. However, percentage abnormality was
lower in T1, comparable in NC and T2, and higher (p<0.05) in PC.
Spermatogenic cell population and epididymal sperm reserve (ESR)
were optimal in control and pretreated animals, while the PC had
lower spermatids and ESR. The concentration of estradiol (EST) was
lower (p<0.05) in the PC and T2, while leuteinizing hormone (LH)
concentration was lower (p<0.05) in the PC, and comparable
(p>0.05) between control and T2. The concentration of
follicle-stimulating hormone (FSH) was comparable (p>0.05) in all
groups, while testosterone (TS) hormone concentration was lower
(p<0.05) in the PC and higher in the control and T1 groups.
Conclusion:
This
study showed the preventive effects of NS administration against
alterations in reproductive hormnes, sperm parameters and gonadal
histology caused by LA in rats.
Keywords:
histology, lead acetate, infertility, Nigella sativa,
spermiogram, toxicity.
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