Open Access
Research
(Published
online: 31-10-2016)
22.
Effect of
Terminalia arjuna
bark powder on some diagnostic enzymes in buffalo (Bubalus
bubalis)
ingesting arsenic contaminated water and
fodder -
Subrat Kumar Dash, Shashi Nayyar and Rajesh Jindal
Veterinary World, 9(10): 1167-1172
doi:
10.14202/vetworld.2016.1167-1172
Subrat Kumar Dash:
Department of Veterinary Physiology and Biochemistry, College of
Veterinary Science, Guru Angad Dev Veterinary and Animal
Sciences University, Ludhiana - 141 004, Punjab, India; dsubrat@ymail.com
Shashi Nayyar:
Department of Veterinary Physiology and Biochemistry, College of
Veterinary Science, Guru Angad Dev Veterinary and Animal
Sciences University, Ludhiana - 141 004, Punjab, India;
shashi.0741@yahoo.com
Rajesh Jindal:
Department of Veterinary Physiology and Biochemistry, College of
Veterinary Science, Guru Angad Dev Veterinary and Animal
Sciences University, Ludhiana - 141 004, Punjab, India;
jindalvpy@yahoo.com
Received: 25-05-2016, Accepted: 20-09-2016, Published online:
31-10-2016
Corresponding author:
Shashi Nayyar, e-mail: shashi.0741@yahoo.com
Citation:
Dash SK, Nayyar S, Jindal R (2016) Effect of
Terminalia arjuna
bark powder on some diagnostic enzymes in buffalo (Bubalus
bubalis)
ingesting arsenic contaminated water and fodder,
Veterinary World, 9(10):
1167-1172.
Abstract
Aim:
The study investigated the effect of
Terminalia arjuna
bark powder on some diagnostic enzymes related to hepatic and
muscle function in buffaloes ingesting arsenic contaminated
water and fodder in an arsenic affected area.
Materials and Methods:
A total of 25 samples of tube well water, fodder and buffalo
blood were collected through a survey from arsenic contaminated
areas and 20 samples from the uncontaminated, i.e., control
areas of Ludhiana district, Punjab for determination of arsenic
concentration. A total of 30 buffaloes (selected from above 45
animals) were divided into three groups of 10 each on the basis
of blood arsenic level,
viz.,
control group: Clinically healthy buffaloes from the
uncontaminated area with the blood arsenic level within the
normal limit (0-0.05 ppm); Arsenic exposed group: Buffaloes
exposed to arsenic through intake of contaminated water and
fodder in the arsenic affected area with the blood arsenic level
above the normal limit of 0-0.05 ppm; treatment group: Arsenic
exposed buffaloes treated with
T. arjuna
bark powder orally at 42 mg/kg b.w. OD for 30 days. Single blood
samples were collected from control and arsenic exposed groups.
Blood samples from the treatment group were collected on 0,
15th, and 30th day of treatment along with one sample on the
45th day, i.e., after withdrawal of treatment. Activities of
alkaline phosphatase (ALP), gamma-glutamyl transferase (GGT),
lactate dehydrogenase (LDH), and creatine kinase (CK) were
assayed in plasma.
Results:
Significantly (p<0.05) higher arsenic concentration was observed
in tube well water, fodder and buffalo blood samples collected
from the arsenic contaminated area. A significant positive
correlation was noticed between arsenic concentrations of tube
well water, fodder and untreated buffalo blood samples,
collected from the arsenic affected area. ALP, GGT, LDH, and CK
activities were significantly (p<0.05) increased in the arsenic
exposed buffaloes compared to control. Treatment with
T. arjuna
bark powder reduced the plasma levels of ALP, GGT, LDH, and CK
in arsenic exposed buffaloes comparable to that of control.
Conclusion:
Exposure to arsenic altered the hepatic and muscle function
enzymes in buffaloes.
T. arjuna
bark powder supplementation lowered the ALP, GGT, LDH, and CK
activities in arsenic exposed buffaloes toward the values
exhibited by the control group.
Keywords:
arsenic,
Bubalus bubalis,
diagnostic enzymes,
Terminalia arjuna.
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