Open Access
Research
(Published
online: 07-10-2016)
3.
Expression of heat-shock protein 72 mRNA
in relation to heart rate variability of Sahiwal and Karan-Fries
in different temperature-humidity indices -
Prava Mayengbam, T. C. Tolenkhomba and R. C. Upadhyay
Veterinary World, 9(10): 1051-1055
doi:
10.14202/vetworld.2016.1051-1055
Prava Mayengbam:
Dairy Cattle Physiology Division, National Dairy Research
Institute, ICAR, Karnal - 132 001, Haryana, India; dr_prava@rediffmail.com
T. C. Tolenkhomba:
Dairy Cattle Physiology Division, National Dairy Research
Institute, ICAR, Karnal - 132 001, Haryana, India; drkhomba10@gmail.com
R. C. Upadhyay:
Dairy Cattle Physiology Division, National Dairy Research
Institute, ICAR, Karnal - 132 001, Haryana, India;
upadhyay.ramesh@gmail.com
Received: 05-04-2016, Accepted: 25-08-2016, Published online:
07-10-2016
Corresponding author:
Prava Mayengbam, e-mail: dr_prava@rediffmail.com
Citation:
Mayengbam P, Tolenkhomba TC, Upadhyay RC (2016) Expression of
heat-shock protein 72 mRNA in relation to heart rate variability
of Sahiwal and Karan-Fries in different temperature-humidity
indices,
Veterinary World, 9(10):
1051-1055.
Abstract
Aim:
To investigate the effect of temperature-humidity index (THI) on
the expression pattern of heat-shock protein 72 (HSP72) mRNA of
Sahiwal and Karan-Fries (KF) cattle in different THIs.
Materials and Methods:
Five different periods of a year were selected based on
combinations of Tmax/Tmin,
viz.,
P1: <20°C/<10°C; P2: >20°C/<10°C, P3: <30°C/<15°C; P4:
>35°C/<20°C, and P5: >35°C/>20°C. The THI was calculated from
the records of temperature and relative humidity in different
periods. Heart rate variability (HRV) was calculated from
electrocardiogram records in different periods. HSP72 mRNA
expression was estimated by reverse transcription polymerase
chain reaction.
Results:
The THI recorded during P1, P2, P3, P4, and P5 were 55.5, 60.3,
70.1, 74.5, and 79.0, respectively. THI in P4 and P5 were
stressful to animals. HSP72 mRNA expression increased during
cold stress in P1 in Sahiwal and heat stress in P4 and P5 in
both Sahiwal and KF. Sahiwal maintained increased HSP72 mRNA
expression longer than KF without causing a significant change
in HRV.
Conclusion:
Both low THI in winter and high THI in summer increased HSP72
mRNA of Sahiwal and KF without significant change in HRV.
Thermotolerance of Sahiwal could be due to the maintenance of
higher HSP72 expression longer than KF in prolonged heat stress
in summer.
Keywords:
heat-shock protein 72 mRNA, Karan-Fries, Karnal, Sahiwal,
stress, temperature-humidity index.
References
1. Sonna, L.A., Jun, F., Stephen, L.G. and Craig, M.L.
(2002) Invited review: Effects of heat and cold stress on
mammalian gene expression. J. Appl. Physiol., 92: 1725-1742.
http://dx.doi.org/10.1152/japplphysiol.01143.2001
PMid:11896043 |
|
|
2. Mishra, S.R. and Palai, T.K. (2014) Importance of HSP70
in livestock - At cellular level. J. Mol. Pathophysiol.,
3(2): 30-32.
http://dx.doi.org/10.5455/jmp.20141028023220 |
|
|
3. Kamwanja, L.A., Chase, C.C.Jr., Gutierrez, J.A.,
Guerriero, V.Jr., Olson, T.A., Hammond, A.C. and Hansen, P.J.
(1994) Responses of bovine lymphocytes to heat shock as
modified by breed and antioxidant status. J. Anim. Sci., 72:
438-444.
PMid:8157528 |
|
|
4. Lacetera, N., Bernabucci, U., Scalia, D., Basirico`, L.,
Morera, P. and Nardone, A. (2006) Heat stress elicits
different responses in peripheral blood mononuclear cells
from Brown-Swiss and Holstein cows. J. Dairy Sci., 89:
4606-4612.
http://dx.doi.org/10.3168/jds.S0022-0302(06)72510-3 |
|
|
5. Patir, H. and Upadhyay, R.C. (2010) Purification,
characterization and expression kinetics of heat shock
protein 70 from Bubalus bubalis. Res. Vet. Sci., 88:
258-262.
http://dx.doi.org/10.1016/j.rvsc.2009.09.004
PMid:19836035 |
|
|
|
6. Prava, M. and Upadhyay, R.C. (2014) Heat shock protein 72
expression of Sahiwal and Karan-Fries during thermal stress.
Indian J. Dairy Sci., 67(2): 147-153. |
|
|
|
7. Prava, M., Upadhyay, R.C. and Tolenkhomba, T.C. (2015)
Seasonal variation in HSP72 expression of Sahiwal and Karan-Fries
cattle. Indian Vet. J., 92(4): 34-36. |
|
|
8. Hahn, G.L., Gaughan, J.B., Mader, T.L. and Eigenberg,
R.A. (2009) Thermal indices and their applications for
livestock environments. In: DeShazer, J.A. and St. Joseph,
M., editors. Livestock Energetics and Thermal Environmental
Management. ASABE, USA. p113-130.
http://dx.doi.org/10.13031/2013.28298 |
|
|
|
9. Mayengbam, P., Tolenkhomba, T.C. and Upadhyay, R.C.
(2015) Mn-SOD and Cu, Zn-SOD mRNA expression in relation to
physiological indices of Sahiwal and Karan-Fries heifers
during different temperature humidity indices. J.
Agrometeorol., 17(2): 172-178. |
|
|
|
10. Choudhary, S.S., Patel, S.B., Singh, V.K. and Manat,
T.D. (2016) Effect of heat stress on HSP72 gene expression
in Surti buffaloes heifers. Souvenir cum Compendium of XXIV
Annual Conference of Society of Animal Physiologists of
India on 21st to 22nd January, 2016 at C.V. Sc., Khanapara,
Guwahati-22. p93. |
|
|
11. Kearl, L.C. (1982) Nutrient Requirements of Ruminants in
Developing Countries. International Feedstuffs Institute,
Utah Agricultural Experiment Station, Utah State University
Logan, Utah, USA.
PMid:7183563 |
|
|
|
12. National Research Council. (1971) A Guide to
Environmental Research on Animals. National Academy of
Science, Washington, DC. p374. |
|
|
13. Task Force of the European Society of Cardiology and the
North American Society of Pacing and Electrophysiology.
(1996) Heart rate variability-standards of measurement,
physiological interpretation, and clinical use. Eur. Heart
J., 17: 354-381.
http://dx.doi.org/10.1093/oxfordjournals.eurheartj.a014868
PMid:8737210 |
|
|
|
14. Corr^ea, G.A., Rumpf, R., Mundim, T.C.D., Franco, M.M.
and Dode, M.A.N. (2007) Oxygen tension during in vitro
culture of bovine embryos: Effect in production and
expression of genes related to oxidative stress. Anim.
Reprod. Sci., 67: 767-775. |
|
|
|
15. Sambrook, J., Fristsch, E.F. and Maniatis, T. (1989)
Molecular Cloning: A Laboratory Manual. 3rd ed., Vol. 1, 2,
3. Cold Spring Harbor, New York. |
|
|
16. Thom, E.C. (1959) The discorfort index. Weatherwise, 12:
57-59.
http://dx.doi.org/10.1080/00431672.1959.9926960 |
|
|
17. Mohr, E., Langbein, J. and Nu¨rnberg, G. (2002) Heart
rate variability- A noninvasive approach to measure stress
in calves and cows. Physiol. Behav., 75: 251-259.
http://dx.doi.org/10.1016/S0031-9384(01)00651-5 |
|
|
18. von Borell, E.V., Langbein, J., Després, G., Hansen, S.,
Leterrier, C., Marchant-Forde, J., Marchant-Forde, R.,
Minero, M., Mohr, E., Prunier, A., Valance, D. and Veissier,
I. (2007) Heart rate variability as a measure of autonomic
regulation of cardiac activity for assessing stress and
welfare in farm animals - A review. Physiol. Behav., 92:
293-316.
http://dx.doi.org/10.1016/j.physbeh.2007.01.007
PMid:17320122 |
|
|
19. Kovács, L., Kézér, F.L., Bakony, M., Hufnágel, L.,
Tőzsér, J. and Jurkovich, V. (2015) Associations between
heart rate variability parameters and housing - and
individual-related variables in dairy cows using canonical
correspondence analysis. PLoS One, 10(12): e0145313.
DOI:10.1371/journal.pone.0145313.
http://dx.doi.org/10.1371/journal.pone.0145313 |
|
|
20. Kovács, L., Kézér, F.L., Jurkovich, V.,
Kulcsár-Huszenicza, M. and Tőzsér, J. (2015) Heart rate
variability as an indicator of chronic stress caused by
lameness in dairy cows. PLoS One, 10(8): e0134792.
DOI:10.1371/journal.pone.0134792.
http://dx.doi.org/10.1371/journal.pone.0134792 |
|
|
|
21. Anton, A. and Solcan, G. (2012) Heart rate variability
for assessing stress in cows. Vet. Med., 58(4): 12-19. |
|
|
22. Zhang, F.J., Weng, G., Wang, J.F., Zhou, D., Zhang, W.,
Zhai, C.C., Hou, Y.X. and Zhu, Y.H. (2014) Temperature –
Humidity index and chromium supplementation on antioxidant
activity, heat shock protein 72, and cytokine response of
lactating cows. J. Anim. Sci., 92: 3026-3034.
http://dx.doi.org/10.2527/jas.2013-6932
PMid:24879765 |
|
|
23. Banerjee, D., Upadhyay, R.C., Chaudhary, U.B., Kumar,
R., Singh, S.V., Ashutosh, Gali, J.M., Polley, S., Mukherjee,
A., Das, T.K. and De, S. (2014) Seasonal variation in
expression pattern of genes under HSP70 family in heat and
cold-adapted goats (Capra hircus). Cell Stress Chaperone.,
19: 401-408.
http://dx.doi.org/10.1007/s12192-013-0469-0
PMid:24114386 PMCid:PMC3982034 |
|
|
24. Collier, R.J., Stiening, C.M., Pollard, B.C., VanBaale,
M.J., Baumgard, L.H., Gentry, P.C. and Coussens, P.M. (2006)
Use of gene expression microarrays for evaluating
environmental stress tolerance at the cellular level in
cattle. J. Anim. Sci., 84 E. Suppl: E1-E13.
http://dx.doi.org/10.2527/2006.8413_supplE1x |
|
|
25. Liu, Y., and Steinacker, J.M. (2001) Changes in skeletal
muscle heat shock proteins: Pathological significance.
Front. Biosci., 6: D12-D25.
http://dx.doi.org/10.2741/a590 |
|
|
26. Kristensen, T.N., Lovendahl, P., Berg, P. and Loeschcke,
V. (2004) Hsp72 is present in plasma from Holstein-Friesian
dairy cattle, and the concentration level is repeatable
across days and age classes. Cell Stress Chaperone., 9:
143-149.
http://dx.doi.org/10.1379/CSC-17.1
PMid:15497501 PMCid:PMC1065294 |
|
|
|
27. Pawar, H.N., Brah, G.S., Agrawal, R.K. and Ramneek.
(2012) Differential expression kinetics of heat shock
protein 70 and associated cytokines between cattle and
buffalo species. J. Cell Tissue Res., 12(2): 3173-3179. |
|
|
|
28. Moloyan, A., Palmon, A. and Horowitz, M. (1999) Heat
acclimation increases the basal HSP72 level and alters its
production dynamics during heat stress. Am. J. Physiol.,
276: R1506-R1515. |
|
|
|
29. Singh, S.V., Vaidya, M.M., Upadhyay, R.C. and Baliyan,
B. (2014) Effect of thermal stress on HSP72 and leptin mRNA
expressions in peri-partiurient dairy cows. 20th
International Congress of Biometeorology on 28th September –
2nd October, 2014 at Cleveland, OH. |
|
|
|
30. Evgen'ev, M.B., Garbug, D.G. and Zatsepina, O.G. (2015)
Adaptive role of Hsps in homeothermal thermophilic
organisms. In: Heat Shock Proteins and Whole Body Adaptation
to Extreme Environments. Springer Dordrecht Heidelberg, New
York, London. p77-78. |
|
