Open Access
Research
(Published
online: 08-10-2016)
4.
Determination of ceruloplasmin,
some other acute phase proteins, and biochemical parameters in
cows with endometritis -
S. Kaya, O. Merhan, C. Kacar, A. Colak
and K. Bozukluhan
Veterinary World, 9(10): 1056-1062
doi:
10.14202/vetworld.2016.1056-1062
S. Kaya :
Department of
Obstetrics and Gynecology, Faculty of Veterinary Medicine,
University of Kafkas, Kars, Turkey; semra-kafkas@hotmail.com
O. Merhan :
Department of
Biochemistry, Faculty of Veterinary Medicine, University of
Kafkas, Kars, Turkey; oguzmerhan@hotmail.com
C. Kacar :
Department of
Obstetrics and Gynecology, Faculty of Veterinary Medicine,
University of Kafkas, Kars, Turkey; cihan3000@hotmail.com
A. Colak :
Department of
Obstetrics and Gynecology, Faculty of Veterinary Medicine,
University of Ataturk, Erzurum, Turkey; fgacolak@hotmail.com
K. Bozukluhan :
Department of
Veterinary Health, Kars School of Higher Vocational Education,
University of Kafkas, Kars, Turkey; kbozukluhan@hotmail.com
Received: 26-05-2016, Accepted: 22-08-2016, Published online:
08-10-2016
Corresponding author:
S. Kaya,
e-mail: semra-kafkas@hotmail.com
Citation:
Kaya S, Merhan O, Kacar C, Colak A, Bozukluhan K (2016)
Determination of ceruloplasmin, some other acute phase proteins,
and biochemical parameters in cows with endometritis,
Veterinary World, 9(10): 1056-1062.
Abstract
Aim:
The aim of
this study is to determine serum ceruloplasmin levels in cows
with endometritis of varying degrees of severity and to
establish whether or not there is a correlation between acute
phase protein (APP) levels and biochemical parameters.
Material and
Methods:
The study was
conducted with 100 Brown Swiss cows (3-8 years of age) on days
28-32 postpartum. Cows were divided into endometritis (mild,
moderate, and severe endometriosis) and healthy groups based on
ultrasonography, vaginoscopy, and cytological examination. Blood
samples were collected from all cows. Levels of haptoglobin
(Hp), serum amyloid A (SAA), ceruloplasmin, albumin, and some
biochemical parameters were analyzed.
Results:
Hp,
SAA, and ceruloplasmin levels were higher in cows with
endometritis than in healthy cows (p=0.001), and the levels of
these APPs increased as endometritis became more severe
(p=0.001). Some significant correlations were found between APPs
and the biochemical parameters that were analyzed. In
conclusion, it was determined that ceruloplasmin levels increase
significantly in the presence of endometritis and proportionate
to the severity of endometritis. A significant correlation was
found between ceruloplasmin levels and Hp and SAA levels.
Conclusion:
It was concluded that ceruloplasmin levels can be used in the
diagnosis of endometritis as an alternative to Hp and SAA
levels.
Keywords:
albumin, ceruloplasmin, cow, endometritis, haptoglobin, serum
amyloid A.
References
1. Cray, C., Zaias, J. and Altman, N.H. (2009) Acute phase
response in animals: A review. Comp. Med., 59: 517-526.
PMid:20034426 PMCid:PMC2798837 |
|
|
2. Biswal, S.S., Das, S., Balasubramanian, S., Mohanty, D.N.,
Sethy, K. and Dasgupta, M. (2014) Serum amyloid A and
haptoglobin levels in crossbred cows with endometritis
following different therapy. Vet. World, 7: 1066-1070.
http://dx.doi.org/10.14202/vetworld.2014.1066-1070 |
|
|
|
3. Tothova, C., Nagy, O. and Kovac, G. (2011) Acute phase
proteins and their use in the diagnosis of diseases in
ruminants: A review. Vet. Med. Czech., 59: 163-180. |
|
|
4. Thomas, F.C., Waterston, M., Hastie, P., Parkin, T.,
Haining, H. and Eckersall, P.D. (2015) The major acute phase
proteins of bovine milk in a commercial dairy herd. BMC Vet.
Res., 11: 207.
http://dx.doi.org/10.1186/s12917-015-0533-3
PMid:26276568 PMCid:PMC4536752 |
|
|
5. Brodzki, P., Kostro, K., Brodzki, A., Wawron, W.,
Marczuk, J. and Kurek, Ł. (2015) Inflammatory cytokines and
acute-phase proteins concentrations in the peripheral blood
and uterus of cows that developed endometritis during early
postpartum. Theriogenology, 84: 11-18.
http://dx.doi.org/10.1016/j.theriogenology.2015.02.006
PMid:25765299 |
|
|
6. Chan, J.P.W., Chu, C.C., Fung, H.P., Chuang, S.T., Lin,
Y.C., Chu, R.M. and Lee, S.L. (2004) Serum haptoglobin
concentration in cattle. J. Vet. Med. Sci., 66: 43-46.
http://dx.doi.org/10.1292/jvms.66.43
PMid:14960809 |
|
|
|
7. Green, M.P., Ledgard, A.M., Berg, M.C., Peterson, A.J.
and Back, P.J. (2009) Prevalence and identification of
systemic markers of sub-clinical endometritis in postpartum
dairy cows. Proc. N. Z. Soc. Anim., 69: 37-42. |
|
|
8. Humblet, M.F., Guyot, H., Boudry, B., Mbayahi, F., Hanzen,
C., Rollin, F. and Godeau, J.M. (2006) Relationship between
haptoglobin, serum amyloid A, and clinical status in a
survey of dairy herds during a 6-month period. Vet. Clin.
Pathol., 35: 188-193.
http://dx.doi.org/10.1111/j.1939-165X.2006.tb00112.x
PMid:16783711 |
|
|
9. Floris, G., Medda, R., Padiglia, A. and Musci, G. (2000)
The physiopathological significance of ceruloplasmin.
Biochem. Pharmacol., 60: 1735-1741.
http://dx.doi.org/10.1016/S0006-2952(00)00399-3 |
|
|
10. Lóvstad, R.A. (2006) A kinetic study on the
phenothiazine dependent oxidation of NADH by bovine
ceruloplasmin. BioMetals, 19: 1-5.
http://dx.doi.org/10.1007/s10534-005-2627-z
PMid:16502325 |
|
|
11. Cerone, S.I., Sansinanea, A.S., Streitenberger, S.A.,
Garcia, M.C. and Auza, N.J. (2000) Cytochrome c oxidase, Cu,
Zn-superoxide dismutase, and ceruloplasmin activities in
Copper-deficient bovines. Biol. Trace. Elem. Res., 73:
269-278.
http://dx.doi.org/10.1385/BTER:73:3:269 |
|
|
|
12. Banuvalli, N., Bhaskaran, R., Krishnamurthy, U., Gruraj,
P.M., Kumar, K.P.H. and Rames, H.S. (2014) Effect of body
condition score at parturition on post-partum productive and
reproductive performance in crossbred dairy cows. Int. J.
Livest. Res., 4: 5-13. |
|
|
13. Weber, C., Hametner, C., Tucscherer, A., Losand, B.,
Kanitz, E., Otten, W., Singh, S.P., Bruckmaier, R.M.,
Becker, F., Kanitz, W. and Hammon, H.M. (2013) Variation in
fat mobilization during early lactation differently affects
feed intake, body condition, and lipid and glucose
methabolism in high-yielding dairy cows. J. Dairy Sci., 96:
165-180.
http://dx.doi.org/10.3168/jds.2012-5574
PMid:23127904 |
|
|
14. Gong, J.G., Lee, W.J., Garnworthy, P.C. and Webb, R.
(2002) Effect of dietary-induced increases in circulating
insulin concentrations during the early postpartum period on
reproductive function in dairy cows. Reproduction, 123:
419-427.
http://dx.doi.org/10.1530/rep.0.1230419
PMid:11882019 |
|
|
15. Liu, P., He, B.X., Yang, X.L., Hou, X.L., Zhao, H.Y.,
Han, Y.H., Nie, P., Deng, H.F. and Cheng, L. (2012)
Activities of aspartate aminotransferase, alanine
aminotransferase, gamma-glutamyl transferase, alkaline
phosphatase in plasma of postpartum Holstein cows. J. Anim.
Vet. Adv., 11: 1270-1274.
http://dx.doi.org/10.3923/javaa.2012.1270.1274 |
|
|
16. Đoković, R., Šamanc, H., Petrović, M.D., Ilić, Z. and
Kurćibić, Z. (2012) Relationship among blood metabolites and
lipid content in the liver in transitional dairy cows.
Biotechnol. Anim. Husb., 28: 705-714.
http://dx.doi.org/10.2298/BAH1204705D |
|
|
17. Burke, C.R., Meier, S., Mcdougall, S., Compton, C.,
Mitchell, M. and Roche, J.R. (2010) Relationships between
endometritis and metabolic state during the transition
period in pasture-grazed dairy cows. J. Dairy Sci., 93:
5363-5373.
http://dx.doi.org/10.3168/jds.2010-3356
PMid:20965352 |
|
|
18. Williams, E.J., Fischer, D.P., Pfeiffer, D.U., England,
G.C.W., Noakes, D.E., Dobson, H. and Sheldon, I.M. (2005)
Clinical evaluation of postpartum vaginal mucus reflects
uterine bacterial infection and the immune response in
cattle. Theriogenology, 63: 102-117.
http://dx.doi.org/10.1016/j.theriogenology.2004.03.017
PMid:15589277 |
|
|
19. Kasimanickam, R., Duffield, T.F., Foster, R.A., Gartley,
C.J., Leslie, K.E., Walton, J.S. and Johnson, W.H. (2004)
Endometrial cytology and ultrasonography for the detection
of subclinical endometritis in postpartum dairy cows.
Theriogenology, 62: 9-23.
http://dx.doi.org/10.1016/j.theriogenology.2003.03.001
PMid:15159097 |
|
|
20. Mari, G., Iacono, E., Toni, F., Predieri, P.G. and
Merlo, B. (2012) Evaluation of the effectiveness of
intrauterine treatment with formosulphathiazole of clinical
endometritis in postpartum dairy cows. Theriogenology, 78:
189-200.
http://dx.doi.org/10.1016/j.theriogenology.2012.01.036
PMid:22464185 |
|
|
21. Edmonson, A.J., Lean, I.J., Weaver, L.D., Farver, T. and
Webster, G. (1989) A body condition score chart for Holstein
dairy cows. J. Dairy Sci., 72: 68-78.
http://dx.doi.org/10.3168/jds.S0022-0302(89)79081-0 |
|
|
22. Skinner, J.G., Brown, R.A. and Roberts, L. (1991) Bovine
haptoglobin response in clinically defined field conditions.
Vet. Rec., 128: 147-149.
http://dx.doi.org/10.1136/vr.128.7.147
PMid:1903006 |
|
|
23. Colombo, J.P. and Richterich, R. (1964) On the
determination of ceruloplasmin in plasma. Schweiz. Med.
Wochenschr., 94: 715-720.
PMid:14242015 |
|
|
24. Gardner, I.A. and Greiner, M. (2006) Receiver-operating
characteristic curves and likelihood ratios: Improvements
over traditional methods for evaluation and application of
veterinary clinical pathology tests. Vet. Clin. Pathol., 35:
8-17.
http://dx.doi.org/10.1111/j.1939-165x.2006.tb00082.x |
|
|
|
25. Biswal, S.S.H., Das, S. and Mohanty, D.N. (2013)
Immunomodulatory effect of oyster glycogen on endometritic
crossbred cows. Indian J. Anim. Reprod., 34: 21-24. |
|
|
26. Chan, J.P.W., Chang, C.C., Hsu, W.L., Liu, W.B. and
Chen, T.H. (2010) Association of increased serum acute-phase
protein concentrations with reproductive performance in
dairy cows with postpartum metritis. Vet. Clin. Pathol., 39:
72-78.
http://dx.doi.org/10.1111/j.1939-165X.2009.00182.x
PMid:19747184 |
|
|
27. Dubuc, J., Duffield, T.F., Leslie, K.E., Walton, J.S.
and Leblanc, S.J. (2010) Risk factors for postpartum uterine
diseases in dairy cows. J. Dairy Sci., 93: 5764-5771.
http://dx.doi.org/10.3168/jds.2010-3429
PMid:21094748 |
|
|
|
28. Alberghina, D., Piccione, G., Casella, S., Panzera, M.,
Morgante, M. and Gianesella, M. (2013) The effect of the
season on some blood metabolites and haptoglobin in dairy
cows during postpartum period. Arch. Tierzucht., 56:
354-359. |
|
|
|
29. Khoshvaghti, A., Nazifi, S., Vafafar, A. and Gheisari,
H.R. (2009) Evaluation of serum and milk haptoglobin in some
inflammatory diseases of cattle. Int. J. Vet. Res., 3:
31-36. |
|
|
30. Hassanpour, A., Gholipour, M. and Rezaie, A. (2011) The
effect of using monensin on haptoglobin serum level in dairy
cattle. J. Anim. Vet. Adv., 10: 1364-1367.
http://dx.doi.org/10.3923/javaa.2011.1364.1367 |
|
|
|
31. Jeremejeva, J., Orro, T. and Kask, K. (2015)
Relationship between acute phase proteins and subsequent
fertility of dairy cows after postpartum uterine
inflammation and healthy cows. Vet. Med. Zoot., 70: 37-41. |
|
|
|
32. Musal, B., Seyrek, K. and Ulutaş, P.A. (2004) Serum
sialic acid, total protein and albumin levels in cows with
metritis treated with intrauterine oxytetracycline. Uludağ
Univ. J. Fac. Vet. Med., 23: 71-75. |
|
|
33. Tóthová, C.S., Nagy, O., Seidel, H., Konvićná, J.,
Farkašová, Z. and Kovác, G. (2008) Acute phase proteins and
variables of protein metabolism in dairy cows during the pre
- and postpartal period. Acta Vet. Brno., 77: 51-57.
http://dx.doi.org/10.2754/avb200877010051 |
|
|
34. Chassagne, M., Barnouin, J. and Chacornac, J.P. (1998)
Biological predictors for early clinical mastitis occurrence
in Holstein cows under field conditions in France. Prev.
Vet. Med., 35: 29-38.
http://dx.doi.org/10.1016/S0167-5877(97)00092-5 |
|
|
35. Szczubiał, M., Dąbrowski, R., Kankofer, M., Bonchniarz,
M. and Komar, M. (2012) Concentration of serum amyloid A and
ceruloplasmin activity in milk from cows with subclinical
mastitis caused by different pathogens. Pol. J. Vet. Sci.,
15: 291-296.
http://dx.doi.org/10.2478/v10181-011-0149-x
PMid:22844707 |
|
|
36. Hussein, H.A. and Staufenbiel, R. (2012) Variations in
copper concentration and ceruloplasmin activity of dairy
cows in relation to lactation stages with regard to
ceruloplasmin to copper ratios. Biol. Trace Elem. Res., 146:
47-52.
http://dx.doi.org/10.1007/s12011-011-9226-3
PMid:22006222 |
|
|
37. Kankofer, M., Albera, E., Feldman, M., Gundling, N. and
Hoedemaker, M. (2010) Comparison of antioxidative/oxidative
profiles in blood plasma of cows with and without retained
fetal placental membranes. Theriogenology, 74: 1385-1395.
http://dx.doi.org/10.1016/j.theriogenology.2010.06.009
PMid:20663543 |
|
|
38. Lamand, M. and Levieux, D. (1981) Effects of infection
on plasma levels of copper and zinc in ewes. Ann. Rech. Vét.,
12: 133-136.
PMid:7200753 |
|
|
39. Talukder, S., Kerrisk, K.L., Gabai, G., Fukutomi, A. and
Celi, P. (2015) Changes in milk oxidative stress biomarkers
in lactating dairy cows with ovulatory and an-ovulatory
oestrous cycles. Anim. Reprod. Sci., 158: 86-95.
http://dx.doi.org/10.1016/j.anireprosci.2015.05.004
PMid:26006094 |
|
|
|
40. Ahmad, I., Lodhi, L.A., Qureshi, Z.I. and Younis, M.
(2004) Studies on blood glucose, total proteins, urea and
cholesterol levels in cyclic, non-cyclic and endometritic
crossbred cows. Pak. Vet. J., 24: 92-94. |
|
|
41. Kaufmann, T.B., Drillich, M., Tenhagen, B.A. and
Heuwieser, W. (2010) Correlations between periparturient
serum concentrations of non-esterified fatty acids,
betahydroxybutyric acid, bilirubin, and urea and the
occurrence of clinical and subclinical postpartum bovine
endometritis. BMC. Vet. Res., 6: 1-6.
http://dx.doi.org/10.1186/1746-6148-6-47
PMid:20979598 PMCid:PMC2988005 |
|
|
42. Heidarpour, M., Mohri, M., Fallah-Rad, A.H., Shahreza,
F.D. and Mohammadi, M. (2012) Acute phase protein
concentration and metabolic status affect the outcome of
treatment in cows with clinical and subclinical
endometritis. Vet. Rec., 171: 219.
http://dx.doi.org/10.1136/vr.100947
PMid:22865113 |
|
