Open Access
Research
(Published
online: 10-10-2016)
5.
Molecular identification of
Mycoplasma synoviae from seroprevalent commercial breeder
farms at Chittagong district, Bangladesh -
Md. Inkeyas Uddin, Md. Harisul Abid, Md. Shafiqul Islam,
Tofazzal Md. Rakib, Ashim Baran Sen, Shah Mohammed Ziqrul Haq
Chowdhury, Md. Nurul Anwar
and Kazi Md. Kamaruddin
Veterinary World, 9(10): 1063-1069
doi:
10.14202/vetworld.2016.1063-1069
Md. Inkeyas Uddin:
Poultry Research
and Training Centre, Chittagong Veterinary and Animal Sciences
University, Khulshi - 4225, Chittagong, Bangladesh;
sarwarprtc@gmail.com
Md. Harisul Abid:
Department of
Livestock Services, People’s Republic of Bangladesh;
harisulabid@yahoo.com
Md. Shafiqul Islam:
Department of
Pathology and Parasitology, Chittagong Veterinary and Animal
Sciences University, Khulshi - 4225, Chittagong, Bangladesh;
si.mamun@ymail.com
Tofazzal Md. Rakib:
Department of
Pathology and Parasitology, Chittagong Veterinary and Animal
Sciences University, Khulshi - 4225, Chittagong, Bangladesh;
rakibtofazzal367@gmail.com
Ashim Baran Sen:
Department of
Livestock Services, People’s Republic of Bangladesh;
asimsen@gmail.com
Shah Mohammed Ziqrul Haq Chowdhury:
Livestock Division, Bangladesh Agricultural Research Council,
Dhaka - 1202, Bangladesh;
ziqrulhaq@gmail.com
Md. Nurul Anwar:
Port City
International University, Chittagong, Bangladesh;
nurulanwar@yahoo.com
Kazi Md. Kamaruddin:
Livestock
Division, Bangladesh Agricultural Research Council, Dhaka -
1202, Bangladesh;
kazikm54@gmail.com
Received: 12-01-2016, Accepted: 26-08-2016, Published online:
10-10-2016
Corresponding author:
Mohammed Inkeyas
Uddin, e-mail: sarwarprtc@gmail.com
Citation:
Uddin MI, Abid MH, Islam MS, Rakib TM, Sen AB, Chowdhury SMZH,
Anwar MN, Kamaruddin KM (2016) Molecular identification of
Mycoplasma synoviae from seroprevalent commercial breeder
farms at Chittagong district, Bangladesh, Veterinary World,
9(10): 1063-1069.
Abstract
Aim:
Worldwide,
Mycoplasma synoviae (MS) is an important pathogen of
poultry, especially for chicken and turkey. It causes
respiratory tract infection and infectious sinusitis. The study
was conducted to determine the seroprevalence of MS infection
with associated risk factors and identification of MS organism
in unvaccinated flocks of commercial breeder farms of the
Chittagong district, Bangladesh.
Materials and
Methods:
A total of 365
serum samples were collected and tested for MS using serum plate
agglutination (SPA) test for determination of MS seroprevalence.
On the other hand, tracheal swabs were collected from each
seropositive flocks for polymerase chain reaction (PCR) to
determine the presence of MS organism.
Results:
Among
the farms, the highest prevalence was found to be 69% and the
lowest prevalence was 28% with the average 60%. The
seroprevalence of MS infection in breeder farms was highest 70%
with the flock size >10,000 birds, whereas it was lowest 57% in
the flocks ranging from 4000 to 7000. According to age group,
the prevalence was found highest 70% in >60 weeks age group of
birds and lowest 42% in 10-19 weeks group. The seroprevalence of
MS in winter season was found as highest as 64%, whereas it was
found lowest 60% in the summer season. There was a statistically
significant difference (p<0.01) among the seroprevalence of MS
in different breeder farms, flock size, and age groups, but
there was no significant (p>0.05) difference in the winter,
summer, and rainy season. To confirm the presence of MS in the
samples, PCR test was applied using specific primers to amplify
a 214 bp region of the 16S rRNA gene of the organism. In PCR,
all seropositive flocks showed a positive result for MS.
Conclusion:
As
the plate agglutination test result showed 100% similar with PCR
result, it can be suggested that agglutination test is better
than molecular and culture techniques for MS detection and it is
also cheaper and less time-consuming method.
Keywords:
breeder farm, Mycoplasma synoviae, polymerase chain
reaction, respiratory infection, risk factors, seroprevalence.
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