Open Access
Research
(Published
online: 14-10-2016)
6.
Polyadenylated tail length variation
pattern in ultra-rapid vitrified bovine oocytes -
D. J. Dutta, Himangshu Raj and Hiramoni Dev
Veterinary World, 9(10): 1070-1074
doi:
10.14202/vetworld.2016.1070-1074
D. J. Dutta:
Department of Veterinary Physiology, Faculty of Veterinary
Science, Assam Agricultural University, Khanapara, Guwahati -
781 022, Assam, India; duttadj@hotmail.com
Himangshu Raj:
Department of Veterinary Physiology, Faculty of Veterinary
Science, Assam Agricultural University, Khanapara, Guwahati -
781 022, Assam, India; xrajxyz@yahoo.co.in
Hiramoni Dev:
Department of Veterinary Physiology, Faculty of Veterinary
Science, Assam Agricultural University, Khanapara, Guwahati -
781 022, Assam, India; drhsd@rediffmail.com
Received: 18-02-2016, Accepted: 28-08-2016, Published online:
14-10-2016
Corresponding author:
D. J. Dutta, e-mail: duttadj@hotmail.com
Citation:
Dutta DJ, Raj H, Dev H (2016) Polyadenylated tail length
variation pattern in ultra-rapid vitrified bovine oocytes,
Veterinary World, 9(10):
1070-1074.
Abstract
Aim:
The current study aims at investigating the polyadenylated (poly[A])
tail length of morphologically high and low competent oocytes at
different developmental stages. Furthermore, effect of
ultra-rapid vitrification on the poly(A) tail length was
studied.
Materials and Methods:
Fresh bovine cumulus oocyte complexes from abattoir originated
ovaries were graded based on morphological characters and
matured
in vitro.
Cryopreservation was done by ultra-rapid vitrification method.
mRNA was isolated from different categories of oocyte and
subjected to ligation-mediated poly(A) test followed by
polymerase chain reaction for determining the poly(A) tail
length of β actin, gap junction protein alpha 1 (GJA1), poly(A)
polymerase alpha (PAPOLA), and heat shock 70 kDa protein (HSP70)
transcripts.
Results:
GJA1, PAPOLA, and HSP70 showed significantly higher poly(A) in
immature oocytes of higher competence irrespective of
vitrification effects as compared to mature oocytes of higher
competence.
Conclusion:
mRNA poly(A) tail size increases in developmentally high
competent immature bovine oocytes. There was limited effect of
ultra-rapid vitrification of bovine oocytes on poly(A).
Keywords:
ligation-mediated poly(A) test, oocyte, poly-adenylated tail,
vitrification.
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