Open Access
Research
(Published
online: 18-09-2016)
12.
Histopathological and immunohistochemical
approaches for the diagnosis of Pasteurellosis in swine
population of Punjab -
Payal Bhat, Nittin Dev Singh, Geeta Devi Leishangthem, Amninder
Kaur, Vishal Mahajan, Harmanjit Singh Banga and Rajinder Singh
Brar
Veterinary World, 9(9): 989-995
doi:
10.14202/vetworld.2016.989-995
Payal Bhat:
Department of Veterinary Pathology, College of Veterinary
Science, Guru Angad Dev Veterinary and Animal Sciences
University, Ludhiana -141 004, Punjab, India; dr.payalbhat@gmail.com
Nittin Dev Singh:
Department of Veterinary Pathology, College of Veterinary
Science, Guru Angad Dev Veterinary and Animal Sciences
University, Ludhiana -141 004, Punjab, India; drndsingh@gmail.com
Geeta Devi Leishangthem:
Animal Disease Research Centre, Guru Angad Dev Veterinary and
Animal Sciences University, Ludhiana- 141 004, Punjab, India;
drgeetapatho@gmail.com
Amninder Kaur:
Department of Veterinary Pathology, College of Veterinary
Science, Guru Angad Dev Veterinary and Animal Sciences
University, Ludhiana -141 004, Punjab, India; dramninder90@gmail.com
Vishal Mahajan:
Animal Disease Research Centre, Guru Angad Dev Veterinary and
Animal Sciences University, Ludhiana- 141 004, Punjab, India;
mahajanv17@gmail.com
Harmanjit Singh Banga:
Department of Veterinary Pathology, College of Veterinary
Science, Guru Angad Dev Veterinary and Animal Sciences
University, Ludhiana -141 004, Punjab, India; bangahs3@yahoo.com
Rajinder Singh Brar:
Department of Veterinary Pathology, College of Veterinary
Science, Guru Angad Dev Veterinary and Animal Sciences
University, Ludhiana -141 004, Punjab, India; rsbrar@email.com
Received: 02-03-2016, Accepted: 06-08-2016, Published online:
18-09-2016
Corresponding author:
Nittin Dev Singh, e-mail: drndsingh@gmail.com
Citation:
Bhat P, Singh ND, Leishangthem GD, Kaur A, Mahajan V, Banga HS,
Brar RS (2016) Histopathological and immunohistochemical
approaches for the diagnosis of Pasteurellosis in swine
population of Punjab,
Veterinary World,
9(9): 989-995.
Abstract
Aim:
Infectious porcine bronchopneumonia, caused by
Pasteurella multocida,
is a widespread disease of major economic significance. Thus,
the aim of the present study was to diagnose swine
Pasteurellosis using gross, histopathological, and
immunopathological approaches in the swine population of Punjab
and to compare the efficacy of immunohistochemical (IHC)
techniques with conventional diagnostic techniques.
Materials and Methods:
A total of 71 adult swine lung samples showing gross pneumonic
changes were collected along with the associated lymph nodes to
carry out the study. The collected samples were then processed
for histopathological and IHC studies.
Results:
Out of the total 71 lung samples, 26 samples were found to be
suspected for Pasteurellosis as per the microscopic changes
observed, and out of these 26 samples, 16 cases were confirmed
to be positive for Pasteurellosis by IHC. Varied macroscopic
changes noted in lungs were pneumonic patches with consolidation
of many lobes, congestion, and focal hemorrhages. Main lesions
associated with lymph nodes were its enlargement and
hemorrhages. Histologically, the lung showed fibrinous and
suppurative bronchopneumonia, multifocal suppuration, thickening
of septa with fibrin combined with cellular infiltration and
edema. The higher IHC expression of
P. multocida
was seen in the bronchial epithelium besides in alveolar and
bronchial exudate. Moreover, on comparing the histopathological
and IHC scores which were calculated on the basis of
characteristic microscopic lesions and number of antigen
positive cells, respectively, a significant positive correlation
(r=0.4234) was found.
Conclusion:
It was concluded that swine population of Punjab is having
P. multocida
infection. The gross and histopathological lesions can be
helpful in the preliminary diagnosis of Pasteurellosis but needs
to be supplemented by other immunodiagnostic tests. Moreover,
IHC technique proved to be a specific, reliable, precise, and
rapid technique to supplement these conventional methods of
diagnosis for Pasteurellosis.
Keywords:
histopathology, immunohistochemical, Pasteurellosis, scoring.
References
|
1. Christensen, G. and Mousing, J. (1992) Respiratory
system. In: Leman, A.D., et al., editors. Diseases of Swine.
7th ed. Iowa State University Press, Ames, Iowa, USA.
p138-162. |
|
|
|
2. Department of Animal Husbandry, Dairying & Fisheries
2011. Annual Report 2011- 12. Department of Animal
Husbandry, Dairying & Fisheries, Ministry of Agriculture,
Government of India, New Delhi. |
|
|
|
3. Mondal, S.K., De, U.K., Das, G.K., Powde, A.M. and Verma,
A.K. (2012) Pattern of mortality of crossbred pigs in an
organized swine production farm. Livest. Sci., 3: 37-44. |
|
|
|
4. Sorensen, V., Jorsal, S.E. and Mousing, J. (2006)
Diseases of the respiratory system. Diseases of Swine. 9th
ed. Iowa State University Press, Ames, Iowa. p149-177. |
|
|
|
5. Christensen, G. and Enoe, C. (1999) The prevalence of
pneumonia, pleuritis, pericarditis and liver 308 spots in
Danish slaugther pigs in 1998, including comparation with
1994. Dan. Vet., 82: 1-12. |
|
|
6. Hansen, M.S., Pors, S.E., Jensen, H.E., Hansen, V.B.,
Bisgaard, M., Flachs, E.M. and Nielsen, O.L. (2010) An
investigation of the pathology and pathogens associated with
porcine respiratory disease 324 complex in Denmark. J. Comp.
Pathol., 143: 120-131.
http://dx.doi.org/10.1016/j.jcpa.2010.01.012
PMid:20181357 |
|
|
7. Cameron, R.D., Oboyle, D., Frost, A.J., Gordon, A.N. and
Fegan, N. (1996) An outbreak of haemorrhagic septicaemia
associated with Pasteurella multocida subsp gallicida in
large pig herd. Aust. Vet. J., 73: 27-29.
http://dx.doi.org/10.1111/j.1751-0813.1996.tb09949.x
PMid:8660187 |
|
|
|
8. Bancroft, J.D. and Gamble, M. (2002) Theory and Practice
of Histological Techniques. 5th ed. Churchill Livingstone
Publisher, Edinburgh. p172-75. |
|
|
|
9. Harish, B.R., Chandranaik, B.M., Venkatesh, M.D.,
Renukaprasad, C., Jayakumar, S.R. and Krishnapp, G. (2005)
An outbreak of swine Pasteurellosis and its pathology.
Indian J. Vet. Pathol., 29(2): 129-130. |
|
|
|
10. Ghosh, R.C., Hirpurkar, S.D. and Mondal, M. (2011) An
outbreak of swine Pasteurellosis in Chhattisgarh. Indian J.
Vet. Pathol., 35(1): 87-88. |
|
|
11. Tigga, M., Ghosh, R.C., Malik, P., Choudhary, B.K.,
Tigga, P. and Nagar, D.K. (2014) Isolation,
characterization, antibiogram and pathology of isolated from
pigs. Vet. World, 7(5): 363-368.
http://dx.doi.org/10.14202/vetworld.2014.363-368 |
|
|
12. Ono, M., Okada, M., Namimatsu, T., Fujii, S., Mukai, T.
and Sakano, T. (2003) Septicaemia and arthritis in pigs
experimentally infected with Pasteurella multocida capsular
serotype A. J. Comp. Pathol., 129(4): 251-8.
http://dx.doi.org/10.1016/S0021-9975(03)00040-9 |
|
|
13. Pors, S.E., Hansen, M.S., Bisgaard, M., Jensen, H.E. and
Iburg, T.M. (2013) Immunohistochemical study of porcine lung
lesions associated with Pasteurella multocida. Vet. J., 197:
483-488.
http://dx.doi.org/10.1016/j.tvjl.2013.03.004
PMid:23683857 |
|
|
14. Cardoso, T.F., Laguna, G.J., Callejo, M., Vela, A.I.,
Carrasco, L., Fernandez, G.J. F., Maldonado, A. and Luque,
I. (2013) Septicaemic Pasteurellosis in free-range pigs
associated with an unusual biovar 13 of Pasteurella
multocida. Vet. Microbiol., 167(3-4): 690-694.
http://dx.doi.org/10.1016/j.vetmic.2013.08.005
PMid:24011940 |
|
|
15. Ciprian, A., Pijoan, C., Cruz, T., Camacho, J., Tortora,
J., Colmenares, G., Lopez-Revilla, R. and Garza, M.D. (1988)
Mycoplasma hyopneumoniae increases the susceptibility of
pigs to experimental Pasteurella multocida. Can. J. Vet.
Res., 52: 434-438.
PMid:3196973 PMCid:PMC1255487 |
|
|
16. Fablet, C., Marois, C., Kuntzsimon, G., Rose, N.,
Dorenlor, V., Eono, F., Eveno, E., Jolly, J.P., Ledevendec,
L., Tocqueville, V., Queguiner, S., Gorin, S., Kobisch, M.
and Madec, F. (2011) Longitudinal study of respiratory
infection patterns of breeding sows in five farrow-to-finish
herds. Vet. Microbiol., 147(3-4): 329-339.
http://dx.doi.org/10.1016/j.vetmic.2010.07.005
PMid:20696539 |
|
|
17. Ross, R.F. (2006) Pasteurella multocida and its role in
porcine pneumonia. Anim. Health Res. Rev., 7: 13-29.
http://dx.doi.org/10.1017/S1466252307001211
PMid:17389051 |
|
|
18. Muller, G. and Kohler, H. (1997) Experimental affecting
of pulmonary clearance of Pasteurella multocida induced
pneumonia in swine. Berl. Munch. Tierarztl Wochenschr, 110:
378-380.
PMid:10084943 |
|
|
|
19. Thacker, E.L. (2006) Mycoplasmal Disease, Diseases of
Swine. 9th ed. Iowa State University Press, Ames. p701-717. |
|
|
20. Ozyildiz, Z., Tel, O.Y., Yilmaz, R., Ozsoy, S.Y. and
Keskin, O. (2013) Pathological and microbiological
investigations of pneumonic pasteurellosis in sheep. J. Fac
Vet Med Univ Kafkas., 19(1): 103-108.
http://dx.doi.org/10.9775/kvfd.2012.7281 |
|
