Open Access
Research
(Published
online: 28-09-2016)
19.
Seroprevalence of contagious ecthyma in
goats of Assam: An analysis by indirect enzyme-linked
immunosorbent assay -
Mousumi Bora, Durlav Prasad Bora, Nagendra Nath Barman,
Biswajyoti Borah and Sutopa Das
Veterinary World, 9(9): 1028-1033
doi:
10.14202/vetworld.2016.1028-1033
Mousumi Bora:
Division of Virology, Indian Veterinary Research Institute,
Izatnagar, Bareilly, Uttar Pradesh, India; mousumeebora11@gmail.com
Durlav Prasad Bora:
Department of Microbiology, College of Veterinary Science, Assam
Agricultural University, Khanapara Campus, Guwahati, Assam,
India; drdpbora@gmail.com
Nagendra Nath Barman:
Department of Microbiology, College of Veterinary Science, Assam
Agricultural University, Khanapara Campus, Guwahati, Assam,
India;
nnbarman@gmail.com
Biswajyoti Borah:
Department of Animal Biotechnology, Assam Agricultural
University, Khanapara Campus, Guwahati, Assam, India;
borah.drbiswajyoti@gmail.com
Sutopa Das:
Department of Microbiology, College of Veterinary Science, Assam
Agricultural University, Khanapara Campus, Guwahati, Assam,
India; d_sutopa@yahoo.com
Received: 10-04-2016, Accepted: 14-08-2016, Published online:
28-09-2016
Corresponding author:
Mousumi Bora, e-mail: mousumeebora11@gmail.com
Citation:
Bora M, Bora DP, Barman NN, Borah B, Das S (2016) Seroprevalence
of contagious ecthyma in goats of Assam: An analysis by indirect
enzyme-linked immunosorbent assay,
Veterinary World, 9(9):
1028-1033.
Abstract
Aim:
The objective of this study was to screen the prevalence of
contagious ecthyma (CE) among the goat population of Assam owing
to its high prevalence rate.
Materials and Methods:
In this study, a total of 231 serum samples were collected from
12 districts of Assam during September 2013 to July 2014. The
serum samples were tested for the presence of antibodies against
Orf virus
(ORFV) by indirect enzyme-linked immunosorbent assay (ELISA).
Indirect ELISA was standardized using purified
Orf
reference virus produced in bulk in primary lamb testes cells.
Results:
Studies on seroprevalence showed 76.62% of goats were
seropositive. The total number of animals were divided into
different age groups starting from 0-2 months, 2-4 months, 4-6
months, and above 8 months and accordingly highest prevalence of
antibodies against ORFV was recorded in the age-group above 8
months of age. Significantly, lower rates of infection were
observed in goats of age group 2-4 months. This study recorded
that seropositivity from naturally infected animals and in
contact apparently healthy animals to be 53.67% and 46.32%,
respectively.
Conclusion:
The results indicated that CE is a prevalent infection in goats
of Assam, and the healthy population is at increased risk of
infection.
Keywords:
Assam, contagious ecthyma, goats, indirect enzyme linked immuno
sorbent assay, serosurveillance.
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