Open Access
Research
(Published
online: 02-09-2016)
2.
Protective and ameliorative effect of sea
buckthorn leaf extract supplementation on lead induced hemato-biochemical
alterations in Wistar rats -
Rizwana Zargar, Pratiksha Raghuwanshi, Ankur Rastogi, Aditi Lal
Koul, Pallavi Khajuria, Aafreen Wahid Ganai and Sumeet Kour
Veterinary World, 9(9): 929-934
doi:
10.14202/vetworld.2016.929-934
Rizwana Zargar:
Division of Veterinary Physiology and Biochemistry, Sher-e-Kashmir
University of Agricultural Sciences and Technology of Jammu, RS
Pura - 181 102, Jammu and Kashmir, India; zargarrizwana@gmail.com
Pratiksha Raghuwanshi:
Division of Veterinary Physiology and Biochemistry, Sher-e-Kashmir
University of Agricultural Sciences and Technology of Jammu, RS
Pura - 181 102, Jammu and Kashmir, India; pratiksha.rastogi@gmail.com
Ankur Rastogi:
Division of Animal Nutrition, Sher-e-Kashmir University of
Agricultural Sciences and Technology of Jammu, RS Pura - 181
102, Jammu and Kashmir, India; dr_ankur76@rediffmail.com
Aditi Lal Koul:
Division of Veterinary Physiology and Biochemistry, Sher-e-Kashmir
University of Agricultural Sciences and Technology of Jammu, RS
Pura - 181 102, Jammu and Kashmir, India; draditi2006@gmail.com
Pallavi Khajuria:
Division of Veterinary Physiology and Biochemistry, Sher-e-Kashmir
University of Agricultural Sciences and Technology of Jammu, RS
Pura - 181 102, Jammu and Kashmir, India; pallavi.khajuria@yahoo.com
Aafreen Wahid Ganai:
Division of Veterinary Public Health and Epidemiology, Sher-e-Kashmir
University of Agricultural Sciences and Technology of Jammu, RS
Pura - 181 102, Jammu and Kashmir, India; 999aafreen@gmail.com
Sumeet Kour:
Division of Veterinary Physiology and Biochemistry, Sher-e-Kashmir
University of Agricultural Sciences and Technology of Jammu, RS
Pura - 181 102, Jammu and Kashmir, India; ssonya558@gmail.com
Received: 08-02-2016, Accepted: 27-07-2016, Published online:
02-09-2016
Corresponding author:
Pratiksha Raghuwanshi, e-mail: pratiksha.rastogi@gmail.com
Citation:
Zargar R, Raghuwanshi P, Rastogi A, Koul AL, Khajuria P, Ganai
AW, Kour S (2016) Protective and ameliorative effect of sea
buckthorn leaf extract supplementation on lead induced hemato-biochemical
alterations in Wistar rats.
Veterinary World, 9(9):
929-934.
Abstract
Aim:
To evaluate the protective and ameliorative effect of aqueous
sea buckthorn leaf extract (SLE) on hemato-biochemical profile
in lead intoxicated Wistar rats.
Materials and Methods:
An experiment was conducted for 60 days. 36 adult male Wistar
rats with a mean body weight of 177.8±12.6 g were divided into
five groups and were subjected to various daily oral treatment
regimens. Group I served as a negative control receiving only
feed and water, Group II (positive control for lead) received
lead acetate at 250 ppm in drinking water, and Group III
(positive control for SLE) received SLE at 100 mg/kg b.wt.
Animals in Group IV received a combination of lead acetate at
250 ppm in drinking water for the first 45 days and SLE at 100
mg/kg b.wt. throughout the experimental period of 60-day, and in
Group V for the last 15 days of the trial after the
administration of lead acetate until the first 45 days of the
trial to study the protective and ameliorating effects of SLE,
respectively. Blood samples were collected from retro-orbital
fossa of each rat on 0th, 45th, and 60th day of the experiment
for hemato-biochemical analysis including hemoglobin (Hb),
packed cell volume (PCV), serum total protein, albumin,
globulin, albumin:globulin ratio, cholesterol, urea, and
creatinine.
Results:
Significantly (p<0.01) lower levels of serum total proteins and
albumin, and a significantly (p<0.01) higher serum cholesterol,
urea and creatinine levels were observed in Group II (lead
intoxicated group) in comparison to Group I (negative control).
Administration of SLE at 100 mg/kg body wt. to lead intoxicated
Wistar rats resulted in normalization of almost all the
biochemical parameters studied in both the treatment Groups,
i.e., IV and V (protective and ameliorative). However, the
effects were more pronounced in the protective group. No effects
of SLE supplementation were observed on Hb levels. PCV levels
improved in protective groups, but no effect was observed in
ameliorative group in comparison to lead intoxicated groups.
Conclusion:
SLE administration at 100 mg/kg b.wt. to lead intoxicated Wistar
rats may be used to protect/ameliorate lead induced biochemical
alterations in Wistar rats.
Keywords:
hemato-biochemical, lead, sea buckthorn leaf extract, Wistar
rats.
References
|
1. Ali, F., Singh, K., Rani, S., Ahirwar, V. and Khan, S.
(2011) Protective effect of Vitamin C against lead acetate
toxicity in blood glucose level of albino rats. IJPI'S. J.
Pharm. Toxicol., 1(1): 6-9. |
|
|
2. Navarro-Moreno, L.G., Quintanar-Escorza, M.A., Gonzalez,
S., Mondragon, R., Cerbon-Solorzano, J., Valdes, J. and
Calderon-Salinas, J.V. (2009) Effects of lead intoxication
on intercellular junctions and biochemical alterations of
renal proximal tubule cells. Toxicol. In Vitro, 23:
1298-1304.
http://dx.doi.org/10.1016/j.tiv.2009.07.020
PMid:19619637 |
|
|
|
3. Abdel-Moneim, A.E., Dkhil, M.A. and Al-Quraishy, S.
(2011) The potential role of flaxseed oil on lead
acetate-Induced kidney injure in adult male albino rats. Afr.
J. Biotechnol., 10(8): 1436-1451. |
|
|
4. Ibrahim, N.M., Eweis, E.A., El-Beltagi, H.S. and
Abdel-Mobdy, Y.E. (2012) Effect of lead acetate toxicity on
experimental male albino rat. Asian Pac. J. Trop. Biomed.,
2: 41-46.
http://dx.doi.org/10.1016/S2221-1691(11)60187-1 |
|
|
5. Nisar, N.A., Sultana, M., Waiz, H.A., Para, P.A., Baba,
N.A., Zargar, F.A. and Raja, W.H. (2013) Experimental study
on the effect of vitamin C administration on lipid
peroxidation and antioxidant enzyme activity in rats exposed
to chlorpyriphos and lead acetate. Vet. World, 5455(10):
461-466.
http://dx.doi.org/10.5455/vetworld.2013.461-466 |
|
|
6. Moreira, E.G., Rosa, G.J.M., Barros, S.B.M., Vassilieff,
V.S. and Vassilieff, I. (2001) Antioxidant defense in rat
brain regions after developmental lead exposure. Toxicology,
169(2): 145-151.
http://dx.doi.org/10.1016/S0300-483X(01)00497-8 |
|
|
7. Soltaninejad, K., Kebriaeezadeh, A., Minaiee, B., Ostad,
S.N., Hosseini, R., Azizi, E. and Abdollahi, M. (2003)
Biochemical and ultrastructural evidences for toxicity of
lead through free radicals in rat brain. Hum. Exp. Toxicol.,
22: 417-423.
PMid:12948081 |
|
|
8. De Marco, M., Halpern, R. and Barros, H.M.T. (2005) Early
behavioral effects of lead perinatal exposure in rat pups.
Toxicology, 211(1-2): 49-58.
http://dx.doi.org/10.1016/j.tox.2005.02.007
PMid:15863247 |
|
|
9. Moreira, E.G., Vassilieff, I. and Vassilieff, V.S. (2001)
Developmental lead exposure: Behavioral alterations in the
short and long term. Neurotoxicol. Teratol., 23: 489-495.
http://dx.doi.org/10.1016/S0892-0362(01)00159-3 |
|
|
10. Bunn, T.L., Parsons, P.J., Kao, E. and Dietert, R.R.
(2001) Exposure to lead during critical windows of embryonic
development: Differential immunotoxic outcome based on stage
of exposure and gender. J. Toxicol. Sci., 64(1): 57-66.
http://dx.doi.org/10.1093/toxsci/64.1.57 |
|
|
11. Bunn, T.L., Parsons, P.J., Kao, E. and Dietert, R.R.
(2001) Gender-based profiles of developmental immunotoxicity
to lead in the rat: Assessment in juveniles and adults. J.
Toxicol. Environ. Health Part A., 64(3): 223-240.
http://dx.doi.org/10.1080/15287390152543708 |
|
|
12. Ercal, N., Neal, R., Treeratphan, P., Lutz, P.M.,
Hammond, T.C., Dennery, P.A. and Spitz, D.R. (2000) A role
for oxidative stress in suppressing serum immunoglobulin
levels in lead-exposed fisher 344 rats. Arch. Environ.
Contam. Toxicol., 39(2): 251-256.
http://dx.doi.org/10.1007/s002440010102
PMid:10871428 |
|
|
13. Razani-Boroujerdi, S., Edwards, B. and Sopori, M.L.
(1999) Lead stimulates lymphocyte proliferation through
enhanced T cell-B cell interaction. J. Pharmacol. Exp. Ther.,
288(2): 714-719.
PMid:9918580 |
|
|
14. Loghman-Adham, M. (1997) Renal effects of environmental
and occupational lead exposure. Environ. Health Perspect.,
105(9): 928-938.
http://dx.doi.org/10.1289/ehp.97105928 |
|
|
15. Patra, R.C., Swarup, D. and Dwivedi, S. (2001)
Antioxidant effects of α- tocopherol, ascorbic acid and L-methionine
on lead-induced oxidative stress to the liver, kidney and
brain in rats. Toxicology, 162(2): 81-88.
http://dx.doi.org/10.1016/S0300-483X(01)00345-6 |
|
|
16. Vargas, I., Castillo, C., Posadas, F. and Escalante, B.
(2003) Acute lead exposure induces renal heme oxygenase-1
and decreases urinary Na+ excretion. Hum. Exp. Toxicol., 2:
237-244.
http://dx.doi.org/10.1191/0960327103ht360oa |
|
|
17. Mousa, H.M., Al-QarawI, A.A., Ali, B.H., Rahman, H.A.A.
and Elmougy, S.A. (2002) Effect of lead exposure on the
erythrocytic antioxidant levels in goats. J. Vet. Med. A.,
49: 531-534.
http://dx.doi.org/10.1046/j.1439-0442.2002.00499.x |
|
|
18. Sivaprasad, R., Nagaraj, M. and Varalakshmi, P. (2003)
Combined efficacies of lipoic acid and meso-2,
3-dimercaptosuccinic acid on lead induced erythrocyte
membrane lipid peroxidation and antioxidant status in rats.
Hum. Exp. Toxicol., 22: 183-192.
http://dx.doi.org/10.1191/0960327103ht335oa
PMid:12755469 |
|
|
19. Ahamed, M., Verma, S., Kumar, A. and Sidiqui, M.K.J.
(2005) Environmental exposure to lead and its co-relation
with biochemical indices in children. Sci. Total Environ.,
346(1): 48-55.
http://dx.doi.org/10.1016/j.scitotenv.2004.12.019
PMid:15993681 |
|
|
20. Flora, S.J.S. (2002) Nutritional components modify metal
absorption. Toxic response and chelation therapy. J. Nutr.
Environ. Med., 12: 51-65.
http://dx.doi.org/10.1080/13590840220123361 |
|
|
21. Aly, A.A. and El-Beltagi, H.E.S. (2010) Influence of
ionizing irradiation on the antioxidant enzyme of Vicia faba
L. Grasa Y Aceite, 61(3): 288-294.
http://dx.doi.org/10.3989/gya.111509 |
|
|
22. El-Beltagi, H.E.S., Salama, Z.A. and El-Hariri, D.M.
(2008) Some biochemical markers for evaluation of flax
cultivars. Under salt stress conditions. J. Nat. Fibers.,
5(4): 316-330.
http://dx.doi.org/10.1080/15440470802252487 |
|
|
23. Ribarov, S.R., Benov, L.C. and Benchev, I.C. (1981) The
effect of lead on haemoglobin catalyzed lipid peroxidation.
J. Biochim. Biophys. Acta, 664: 453-459.
http://dx.doi.org/10.1016/0005-2760(81)90123-5 |
|
|
|
24. El Kader, M.A.A., El-Sammad, N.M. and Taha, H. (2012)
The protective role of rosemary (Rosmarinus officinalis) in
lead acetate induced toxicity in rats. J. Appl. Sci. Res.,
8: 3071-3082. |
|
|
25. Geetha, S., Sai Ram, S.M., Mongia, S.S., Singh, V.,
Ilavazhagan, G. and Sawhney, R.C. (2003) Evaluation of
antioxidant activity of leaf extract of seabuckthorn
(Hippophae rhamnoides L.) on chromium induced oxidative
stress in albino rats. J. Ethnopharmacol., 87: 247-251.
http://dx.doi.org/10.1016/S0378-8741(03)00154-5 |
|
|
26. Geetha, S., Sai Ram, S.M., Mongia, S.S., Singh, V.,
Ilavazhagan, G. and Sawhney, R.C. (2002) Antioxidant and
immunomodulatory properties of seabuckthorn (Hippophae
rhamnoides) and in vitro study. J. Ethnopharmacol., 79:
373-378.
http://dx.doi.org/10.1016/S0378-8741(01)00406-8 |
|
|
27. Geetha, S. and Gupta, A. (2011) Medicinal and
therapeutic potential of seabuckthorn (Hippophae rhamnoides
L.). J. Ethnopharmacol., 138: 268-278.
http://dx.doi.org/10.1016/j.jep.2011.09.024
PMid:21963559 |
|
|
28. Saggu, S. and Kumar, R. (2008) Effect of seabuckthorn
leaf extracts on circulating energy fuels, lipid
peroxidation and antioxidant parameters in rats during
exposure to cold, hypoxia and restraint (C-H-R) stress and
post stress recovery. Phytomedicine, 15: 437-446.
http://dx.doi.org/10.1016/j.phymed.2007.11.002 |
|
|
|
29. Kumar, A., Tyagi, S.P., Dogra, R. and Gupta, S. (2014)
Dose dependent effects of seabuckthorn seed oil on the
healing of gastric ulcers and erosions in dogs. In: Morsel,
J.T., Zubarev, Y. and Eagle, D., editors. Seabuckthorn:
Research for a Promising Crop. BoD: Books on Demand
Norderstedt, Berlin, p117-124. |
|
|
30. Dubey, S., Deep, P. and Singh, A.K. (2016) Phytochemical
characterization and evaluation of anticatarct potential of
seabuckthorn leave extract. Vet. Opthamol., 19: 144-148.
http://dx.doi.org/10.1111/vop.12271
PMid:25833733 |
|
|
31. Saggu, S. and Kumar, R. (2007) Effect of seabuckthorn
leaf extracts on circulating energy fuels, lipid
peroxidation and antioxidant parameters in rats during
exposure to cold, hypoxia and restraint (C-H-R) stress and
post stress recovery. Phytomedicine, 15: 437-446.
http://dx.doi.org/10.1016/j.phymed.2007.11.002
PMid:18162386 |
|
|
|
32. Dacie, J.V. and Lewis, S.M. (1968) Practical
Haematology. Churchill Livingstone, London. |
|
|
33. ICSH. (1978) Protocol for type testing equipment and
apparatus used for haematological analysis. J. Clin.
Pathol., 31: 275-279.
http://dx.doi.org/10.1136/jcp.31.3.275
PMCid:PMC1145243 |
|
|
|
34. Snedecor, G.W. and Cochran, W.G. (1994) Statistical
Methods. 8th ed. East West Press Private Limited, New Delhi. |
|
|
35. Duncan, D.B. (1955) Multiple range and multiple 'F'
test. Biometrics, 11: 1-42.
http://dx.doi.org/10.2307/3001478 |
|
|
|
36. Helmy, M.A., Elnaga, N.I. and Hela, S.M. (2000) Effect
of administration of milk and kareish cheese on
hematological values and histopathological changes in liver
and brains of rat treated with lead. Alex. J. Agric. Res.,
45: 103-115. |
|
|
37. Shah, F., Kazi, T.G., Afridi, H.I., Baig, J.A., Sumaira,
K., Kolachi, N.F., Wadhwa, S.K. and Shah, A.Q. (2010)
Environmental exposure of lead and iron deficit anemia in
children age ranged 1-5 years: A cross sectional study. Sci.
Total Environ., 408: 5325-5330.
http://dx.doi.org/10.1016/j.scitotenv.2010.07.091
PMid:20801490 |
|
|
|
38. Sharma, R., Panwar, K. and Mogra, S. (2012) Effects of
prenatal and neonatal exposure to lead on white blood cells
in Swiss mice. J. Cell Mol. Biol., 10(1): 33-40. |
|
|
|
39. Azab, A.E.A., El-Dakhly, A.T., Alrawi, Q.K. and Albasha,
M.O. (2013) Protective effects of sesame oil against lead
acetate induced haemato-biochemical toxicity in albino mice.
Int. J. Sci. Res., 4(2): 2053-2063. |
|
|
|
40. Suradkar, S.G., Ghodasara, D.J., Patel, P.J., Jaiswal,
V. and Prajapati, K.S. (2009) Hemato biochemical alterations
induced by lead acetate toxicity in wistar rats. Vet. World,
2(11): 429-439. |
|
|
41. Suryakumar, G. and Gupta, A. (2011) Medicinal and
therapeutic potential of Seabuckthorn (Hippophae rhamnoides
L.). J. Ethnopharmacol., 138: 268-278.
http://dx.doi.org/10.1016/j.jep.2011.09.024
PMid:21963559 |
|
|
|
42. Kansal, L., Sharma, V., Sharma, A., Lodi, S. and Sharma,
S.H. (2011) Protective role of coriandrum sativum
(coriander) extracts against lead nitrate induced oxidative
stress and tissue damage in the liver and kidney in male
mice. Int. J. Appl. Biol. Pharm., 2: 65-83. |
|
|
|
43. Kaneko, J.J., Harvey, J.W. and Bruss, M.L. (1997)
Clinical Biochemistry of Domestic Animals. 5th ed. Academic
Press, San Diego, California, USA. |
|
|
|
44. Sharaf, N.E., Zaki, M.S., Gomma, W.R. and Fawzi, O.M.
(2008) Some clinicopathological and microbiological studies
on lead toxicity in bulls. Am. Euras. J. Agric. Environ.
Sci., 3: 165-168. |
|
|
|
45. Goran, G.V., Crivineanu, V., Papuc, C. and Crivineanu,
C.D. (2008) Effects of sea-buckthorn alcoholic extracts
(Hippophae fructus) on hepatic and renal functions in
laboratory rat. Vet. Med., 65: 288-292. |
|
|
|
46. Sharma, A., Hirulkar, N.B., Wadel, P. and Das, P. (2011)
Influence of hyperglycemia on renal function parameters in
patients with diabetes mellitus. Int. J. Pharm. Biol. Arch.,
2(2): 734-739. |
|
|
47. Mohamed, N.E. and Wakwak, M.M. (2014) Effect of sesame
seeds or oil supplementation to the feed on some
physiological parameters in Japanese quail. J. Radiat. Res.
Appl. Sci., 7: 101-109.
http://dx.doi.org/10.1016/j.jrras.2013.12.003 |
|
|
48. Karamala, S.K., Sri Latha, C.H., Anjaneyulu, Y., Rao,
T.S.C., Vasulu, D.S. and Pidugu, A.P. (2011)
Hematobiochemical changes of lead poisoning and amelioration
with Ocimum sanctum in wistar albino rats. Vet. World, 4:
260-263.
http://dx.doi.org/10.5455/vetworld.4.260 |
|
|
|
49. Saka, S., Bahi, A. and Aouacheri, W. (2011) The effect
of oxidative stress induced by lead acetate on the
glutathione enzymatic system in rats. Ann. Toxicol. Anal.,
23: 1-7. |
|
|
|
50. Aissi, A.K., Fah, L., Akpovi, C.D., Klotoé, J.R.,
Dougnon, V.T., Guédénon, P., Edorh, P.A. and Loko, F. (2014)
Impact of simultaneous exposure to lead and efavirenz on
some biochemical markers in wistar rats. J. Environ. Anal.
Toxicol., 4: 1-5. |
|
|
51. Hardie, D.G. (2011) AMP - Activated protein kinase - An
energy sensor that regulates all aspects of cell function.
Genes. Dev., 25: 1895-1908.
http://dx.doi.org/10.1101/gad.17420111
PMid:21937710 PMCid:PMC3185962 |
|
|
52. Ambali, S.F., Ayo, J.O., Ojo, S.A. and Esievo, K.A.N.
(2011) Ameliorative effect of vitamin C on chlorpyrifos-induced
increased erythrocyte fragility in wistar rats. Hum. Exp.
Toxicol., 30: 19-24.
http://dx.doi.org/10.1177/0960327110368415
PMid:20378658 |
|
|
53. Abdou, H.M. and Hassan, M.A. (2014) Protective role of
omega-3 polyunsaturated fatty acid against lead
acetate-induced toxicity in liver and kidney of female rats.
Bio. Med. Res. Int., 10: 1-11.
http://dx.doi.org/10.1155/2014/435857
PMCid:PMC4086517 |
|
|
54. Ugbaja, N.R., Onunkwor, B.O. and Omoniyi, A.D. (2013)
Lead induced dyslipidemia: The comparative effects of
ascorbate and chelation therapy. Afr. J. Biotechnol., 12:
1845-1852.
http://dx.doi.org/10.5897/AJB2012.2982 |
|
