Vet World   Vol.10   August-2017  Article-16

Research Article

Veterinary World, 10(8): 927-931

https://doi.org/10.14202/vetworld.2017.927-931

Prevalence of Listeria monocytogenes, Yersinia enterocolitica, Staphylococcus aureus, and Salmonella enterica Typhimurium in meat and meat products using multiplex polymerase chain reaction

C. Latha, C. J. Anu, V. J. Ajaykumar, and B. Sunil
Department of Veterinary Public Health, College of Veterinary and Animal Sciences, Mannuthy, Thrissur - 680 651, Kerala, India.

Background and Aim: The objective of the study was to investigate the occurrence of Listeria monocytogenes, Yersinia enterocolitica, Staphylococcus aureus, and Salmonella enterica Typhimurium in meat and meat products using the multiplex polymerase chain reaction (PCR) method.

Materials and Methods: The assay combined an enrichment step in tryptic soy broth with yeast extract formulated for the simultaneous growth of target pathogens, DNA isolation and multiplex PCR. A total of 1134 samples including beef (n=349), chicken (n=325), pork (n=310), chevon (n=50), and meat products (n=100) were collected from different parts of Kerala, India. All the samples were subjected to multiplex PCR analysis and culture-based detection for the four pathogens in parallel.

Results: Overall occurrence of L. monocytogenes was 0.08 % by cultural method. However, no L. monocytogenes was obtained by multiplex PCR method. Yersinia enterocolitica was obtained from beef and pork samples. A high prevalence of S. aureus (46.7%) was found in all types of meat samples tested. None of the samples was positive for S. Typhimurium.

Conclusion: Multiplex PCR assay used in this study can detect more than one pathogen simultaneously by amplifying more than one target gene in a single reaction, which can save time and labor cost. Keywords: food borne pathogens, multiplex polymerase chain reaction, prevalence.

Keywords: food borne pathogens, multiplex polymerase chain reaction, prevalence.

How to cite this article: Latha C, Anu CJ, Ajaykumar VJ, Sunil B (2017) Prevalence of Listeria monocytogenes, Yersinia enterocolitica, Staphylococcus aureus, and Salmonella enterica Typhimurium in meat and meat products using multiplex polymerase chain reaction, Veterinary World, 10(8): 927-931.

Received: 28-03-2017  Accepted: 11-07-2017     Published online: 16-08-2017

Corresponding author: C. Latha   E-mail: latha@kvasu.ac.in

DOI: 10.14202/vetworld.2017.927-931

Copyright: Latha, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.