Open Access
Research
(Published
online: 05-02-2017)
2.
Characterization of Salmonella
Gallinarum from an outbreak in Raigarh, Chhattisgarh -
Chandrahas Sannat, Anil Patyal, Nidhi Rawat, R. C. Ghosh, D. K.
Jolhe, R. K. Shende, S. D. Hirpurkar
and Sanjay Shakya
Veterinary World, 10(2): 144-148
doi:
10.14202/vetworld.2017.144-148
Chandrahas Sannat :
Department of
Veterinary Microbiology, College of Veterinary Science & Animal
Husbandry, Chhattisgarh Kamdhenu Vishwavidyalaya, Anjora, Durg -
491 001, Chhattisgarh, India.
Anil Patyal :
Department of
Veterinary Public Health, College of Veterinary Science & Animal
Husbandry, Chhattisgarh Kamdhenu Vishwavidyalaya, Anjora, Durg -
491 001, Chhattisgarh, India.
Nidhi Rawat :
Department of
Veterinary Microbiology, College of Veterinary Science & Animal
Husbandry, Chhattisgarh Kamdhenu Vishwavidyalaya, Anjora, Durg -
491 001, Chhattisgarh, India.
R. C. Ghosh :
Department of
Veterinary Pathology, College of Veterinary Science & Animal
Husbandry, Chhattisgarh Kamdhenu Vishwavidyalaya, Anjora, Durg -
491 001, Chhattisgarh, India.
D. K. Jolhe :
Department of
Veterinary Pathology, College of Veterinary Science & Animal
Husbandry, Chhattisgarh Kamdhenu Vishwavidyalaya, Anjora, Durg -
491 001, Chhattisgarh, India.
R. K. Shende :
Department of
Veterinary Microbiology, College of Veterinary Science & Animal
Husbandry, Chhattisgarh Kamdhenu Vishwavidyalaya, Anjora, Durg -
491 001, Chhattisgarh, India.
S. D. Hirpurkar :
Department of
Veterinary Microbiology, College of Veterinary Science & Animal
Husbandry, Chhattisgarh Kamdhenu Vishwavidyalaya, Anjora, Durg -
491 001, Chhattisgarh, India.
Sanjay Shakya :
Department of
Veterinary Public Health, College of Veterinary Science & Animal
Husbandry, Chhattisgarh Kamdhenu Vishwavidyalaya, Anjora, Durg -
491 001, Chhattisgarh, India.
Received: 15-08-2016, Accepted: 31-12-2016, Published online:
05-02-2017
Corresponding author:
Chandrahas Sannat,
e-mail: csannat@rediffmail.com
Citation:
Sannat C, Patyal A, Rawat N, Ghosh RC, Jolhe DK, Shende RK,
Hirpurkar SD, Shakya S (2017) Characterization of Salmonella
Gallinarum from an outbreak in Raigarh, Chhattisgarh,
Veterinary World, 10(2): 144-148.
Abstract
Aim:
The present
investigation was conducted to isolate and characterize
Salmonella Gallinarum from an outbreak of fowl typhoid in
layer birds.
Materials and
Methods:
Clinically ill and
dead layer birds from an outbreak were investigated. History,
clinical signs, and postmortem lesions were suggestive of fowl
typhoid. Postmortem samples including heart blood, intestinal
contents, pieces of ovary, and liver were collected and processed
immediately for bacterial culture, serotyping and antibiotic
sensitivity tests. Isolates were further screened for the presence
of extended spectrum beta lactamase (ESBL) (blaTEM)
gene by polymerase chain reaction.
Results:
On the basis
of cultural, staining and biochemical characteristics; three
bacterial isolates were confirmed as S. Gallinarum. On
serotyping, somatic antigen O: 9 and 12 with nonflagellated
antigen were detected in all three isolates. Isolates were
intermediate sensitive to amoxycillin, amoxyclav, gentamicin and
ciprofloxacin and resistant to most of the antibiotics including
chloramphenicol, ampicillin, ceftazidime, cefexime, cefepime,
azithromycin, nalidixin, tetracycline, oxytetracycline, and
streptomycin. Two isolates were found to harbor ESBL (blaTEM)
gene.
Conclusion:
Beta
lactamase producer S. Gallinarum was confirmed as cause of
increased mortality in layer birds during present investigation.
Existence of multi drug resistant Salmonella poses serious
threat to poultry industry in Chhattisgarh.
Keywords:
blaTEM,
O: 9; 12, poultry, Salmonella Gallinarum.
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