Open Access
Research
(Published
online: 19-01-2017)
12.
Protective effect of rutin in comparison to
silymarin against induced hepatotoxicity in rats -
M. Kasi Reddy, A. Gopala Reddy, B. Kala Kumar, D. Madhuri, G.
Boobalan and M. Anudeep Reddy
Veterinary World, 10(1): 74-80
doi:
10.14202/vetworld.2017.74-80
M. Kasi Reddy:
Department of Veterinary Pharmacology and Toxicology, P V
Narsimha Rao Telangana Veterinary University, College of
Veterinary Science, Rajendranagar, Hyderabad - 500 030,
Telangana, India.
A. Gopala Reddy:
Department of Veterinary Pharmacology and Toxicology, P V Narsimha
Rao Telangana Veterinary University, College of Veterinary
Science, Rajendranagar, Hyderabad - 500 030, Telangana, India.
B. Kala Kumar:
Department of Veterinary Pharmacology and Toxicology, P V Narsimha
Rao Telangana Veterinary University, College of Veterinary
Science, Rajendranagar, Hyderabad - 500 030, Telangana, India.
D. Madhuri:
Department of Veterinary Pathology, P V Narsimha Rao Telangana
Veterinary University, College of Veterinary Science,
Rajendranagar, Hyderabad - 500 030, Telangana, India.
G. Boobalan:
Department of Veterinary Pharmacology and Toxicology, P V Narsimha
Rao Telangana Veterinary University, College of Veterinary
Science, Rajendranagar, Hyderabad - 500 030, Telangana, India.
M. Anudeep Reddy:
Department of Veterinary Pharmacology and Toxicology, P V Narsimha
Rao Telangana Veterinary University, College of Veterinary
Science, Rajendranagar, Hyderabad - 500 030, Telangana, India.
Received: 21-09-2016, Accepted: 16-12-2016, Published online:
19-01-2017
Corresponding author:
M. Anudeep Reddy, e-mail: anudeepreddy07@gmail.com
Citation:
Reddy MK, Reddy AG, Kumar BK, Madhuri D, Boobalan G, Reddy MA
(2017) Protective effect of rutin in comparison to silymarin
against induced hepatotoxicity in rats,
Veterinary World, 10(1):
74-80.
Abstract
Aim:
The aim of this study is to evaluate the hepatoprotective effect
of rutin (RTN) in comparison to silymarin (SLM) against
acetaminophen (APAP)-induced hepatotoxicity in rats.
Materials and Methods:
Male Wistar albino rats (n=24) of 3 months age were equally
divided into four groups. Group 1 served as normal control.
Hepatotoxicity was induced in the remaining three groups with
administration of 500 mg/kg po APAP from day 1-3. Groups 2, 3, and
4 were subsequently administered orally with distilled water, 25
mg/kg of SLM, and 20 mg/kg of RTN, respectively, for 11 days. The
mean body weights and biomarkers of hepatotoxicity were estimated
on day 0, 4 (confirmation of toxicity), and 15 (at the end of
treatment). Hematological parameters were evaluated on day 4 and
15. Antioxidant profile and adenosine triphosphatases (ATPases)
were assessed at the end of the experiment. Liver tissues were
subjected to histopathology and transmission electron microscopy
after the sacrifice on day 15.
Results:
Antioxidant profile, ATPases, and hematological and sero-biochemical
parameters were significantly altered, and histopathological
changes were noticed in the liver of toxic control group. These
changes were reversed in groups 3 and 4 that were administered
with SLM and RTN, respectively.
Conclusion:
The results of the present investigation enunciated that SLM has
potent hepatoprotective activity though the RTN was found superior
in restoring the pathological alterations in paracetamol-induced
hepatotoxicity in Wistar albino rats.
Keywords:
acetaminophen, hepatotoxicity, rutin, silymarin.
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