Open Access
Research
(Published
online: 27-01-2017)
18.
Nutrigenomic evaluation of garlic (Allium
sativum)
and holy basil (Ocimum
sanctum)
leaf powder supplementation on growth performance and immune
characteristics in broilers -
N. Sheoran, R. Kumar, A. Kumar, K. Batra, S. Sihag, S. Maan and N.
S. Maan
Veterinary World, 10(1): 121-129
doi:
10.14202/vetworld.2017.121-129
N. Sheoran:
Department of Animal Nutrition, College of Veterinary Sciences,
LLR University of Veterinary and Animal Sciences, Hisar - 125
004, Haryana, India.
R. Kumar:
Department of Animal Nutrition, College of Veterinary Sciences,
LLR University of Veterinary and Animal Sciences, Hisar - 125 004,
Haryana, India.
A. Kumar:
Department of Animal Biotechnology, College of Veterinary
Sciences, LLR University of Veterinary and Animal Sciences, Hisar
- 125 004, Haryana, India.
K. Batra:
Department of Animal Biotechnology, College of Veterinary
Sciences, LLR University of Veterinary and Animal Sciences, Hisar
- 125 004, Haryana, India.
S. Sihag:
Department of Animal Nutrition, College of Veterinary Sciences,
LLR University of Veterinary and Animal Sciences, Hisar - 125 004,
Haryana, India.
S. Maan:
Department of Animal Biotechnology, College of Veterinary
Sciences, LLR University of Veterinary and Animal Sciences, Hisar
- 125 004, Haryana, India.
N. S. Maan:
Department of Animal Nutrition, College of Veterinary Sciences,
LLR University of Veterinary and Animal Sciences, Hisar - 125 004,
Haryana, India.
Received: 31-08-2016, Accepted: 28-12-2016, Published online:
27-01-2017
Corresponding author:
N. S. Maan, e-mail: narendermaan108@gmail.com
Citation:
Sheoran N, Kumar R, Kumar A, Batra K, Sihag S, Maan S, Maan NS
(2017) Nutrigenomic evaluation of garlic (Allium
sativum)
and holy basil (Ocimum
sanctum)
leaf powder supplementation on growth performance and immune
characteristics in broilers,
Veterinary World,
10(1): 121-129.
Abstract
Aim:
In this study, a planned research work was conducted to
investigate the nutrigenomic aspects of supplementation of
Allium sativum
(garlic) and
Ocimum sanctum
(holy basil) leaf powder on the growth performance and immune
characteristics of broilers.
Materials and Methods:
A
6 weeks feeding trial was conducted with 280-day-old Ven Cobb
broilers, distributed randomly into seven experimental groups.
Each treatment had 4 replicates with 10 birds each. The birds of
the control group (T1) were fed a basal diet formulated as per BIS
standards. The broilers of treatment groups T2 and T3 were fed
basal diet supplemented with the commercially available garlic
powder (GP) at levels of 0.5% and 1.0% of the feed, respectively,
while broilers in T4 and T5 were fed basal diet supplemented with
commercial grade holy basil leaf powder (HBLP) at levels 0.5% and
1.0% of the feed, respectively. Birds in the T6 were fed with 0.5%
GP and 0.5% HBLP, whereas T7 was fed with 1.0% GP and 1.0% HBLP.
At the end of the feeding trial (6th week), blood samples were
collected and analyzed for relative mRNA expression of toll-like
receptors (TLR) 2, TLR 4 and TLR 7 using real-time polymerase
chain reaction.
Results:
The mean body weight gain and feed conversion efficiency were
improved (p<0.05) in broilers fed the GP and HBLP incorporated
diets compared with the control group. The relative mRNA
expression levels of TLR 2, TLR 4 and TLR 7 in the peripheral
blood of the broilers were found to be increased (p<0.05) in the
birds supplemented with graded levels of the GP and HBLP as
compared to the untreated group.
Conclusion:
The present work concludes that the inclusion of GP and HBLP could
enhance the production performance and immune status of birds by
augmenting the T-cell mediated immune response and thereby
protects them from disease without decreasing growth traits as a
possible substitution to conventional antimicrobials.
Keywords:
broilers, garlic, gene expression, holy basil, toll-like
receptors.
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