Semen characteristics, extension, and cryopreservation of Rusa deer (Rusa timorensis)

Vet World Vol.10 July-2017 Article-12

Research Article

Veterinary World, 10(7): 779-785

https://doi.org/10.14202/vetworld.2017.779-785

Wan-Nor Fitri¹, Haron Wahid^1,2, Yusoff Rosnina¹, Faez Firdaus Abdullah Jesse^1,3, Zainal Abidin Aimi-Sarah⁴, Mohd Lila Mohd-Azmi⁵, Che' Amat Azlan¹, Muhammad Rashid Azrolharith⁶, Innocent Damudu Peter¹, and Falah Hasan Ali Baiee¹

1. Department of Veterinary Clinical Studies, Universiti Putra Malaysia, 43400 UPM, Serdang, Selangor, Malaysia.
2. Research Centre for Wildlife, Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400 UPM, Serdang, Selangor, Malaysia.
3. Research Centre for Ruminant Diseases, Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400 UPM, Serdang, Selangor, Malaysia.
4. Food Science and Technology Program School of Industrial Technology, Faculty of Applied Science, Universiti Teknologi Mara (UiTM), 40450 Shah Alam, Selangor, Malaysia.
5. Department of Veterinary Pathology and Microbiology, Universiti Putra Malaysia, 43400 UPM, Serdang, Selangor, Malaysia.
6. Department of Medicine & Surgery of Farm & Exotic Animal, Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400 UPM, Serdang, Selangor, Malaysia.

Background and Aim: The objective of this research is to report parameters for breeding soundness evaluation, semen extension, and cryopreservation in Rusa timorensis.

Materials and Methods: Seven healthy stags were chosen for semen collection using an electroejaculator. The collections were performed twice in a breeding season between February and June 2016. Samples were collected between 2 and 3 weeks interval, collected twice for each animal. Semen was evaluated, extended, and cryopreserved using four different extenders; Andromed®, BioXcell®, Triladyl®, and a modified Tris-egg yolk combined with Eurycoma longifolia Jack.

Results: R. timorensis semen characteristics according to volume (ml), color, sperm concentration (106/ml), general motility (%), progressive motility (%), and % morphology of normal spermatozoa are 0.86±0.18 ml, thin milky to milky, 1194.2±346.1 106/ml, 82.9±2.8%, 76.1±4.8%, and 83.9±4.8%, respectively.

Conclusion: Semen characteristics of R. timorensis collected by electroejaculation is good allowing for cryopreservation and future artificial insemination work. The most suitable extender for Rusa deer semen is Andromed®. Keywords: cryopreservation, electroejaculation, extension, Rusa deer, semen characteristics.

Keywords: cryopreservation, electroejaculation, extension, Rusa deer, semen characteristics.

How to cite this article: Fitri WN, Wahid H, Rosnina Y, Jesse FFA, Aimi-Sarah ZA, Mohd-Azmi ML, Azlan CA, Azrolharith MR, Peter ID, Baiee FHA (2017) Semen characteristics, extension, and cryopreservation of Rusa deer (Rusa timorensis), Veterinary World, 10(7): 779-785.

Received: 29-11-2016 Accepted: 01-06-2017 Published online: 15-07-2017

Corresponding author: Haron Wahid E-mail: wahidh@upm.edu.my

DOI: 10.14202/vetworld.2017.779-785

Copyright: Fitri, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.