Characterization of Salmonella Gallinarum isolates from backyard poultry by polymerase chain reaction detection of invasion (invA) and Salmonella plasmid virulence (spvC) genes

Vet World Vol.10 July-2017 Article-18

Research Article

Veterinary World, 10(7): 814-817

https://doi.org/10.14202/vetworld.2017.814-817

Susmita Pal, Samir Dey, Kunal Batabyal, Abhiroop Banerjee, Siddhartha Narayan Joardar, Indranil Samanta, and Devi Prasad Isore

Department of Veterinary Microbiology, West Bengal University of Animal and Fishery Sciences, Belgachia, Kolkata - 700 037, West Bengal, India.

Background and Aim: The aim was to characterize Salmonella enterica serovar Gallinarum isolated from backyard poultry by polymerase chain reaction (PCR) detection of virulence genes invasion (invA) and Salmonella plasmid virulence C (spvC).

Materials and Methods: Two strains of Salmonella serovar Gallinarum isolates used in this study were obtained from an outbreak of fowl typhoid in backyard Vanaraja fowl. PCR technique was used for detection of invA and spvC genes using standard methodology. The invA PCR product from one representative isolate was sequenced and compared with other related Salmonella serovars in GenBank data.

Results: Salmonella Gallinarum produced expected amplicons of invA and spvC gene products. Nucleotide sequence of 285 bp invA gene was deposited in GenBank with accession no. KX788214. Sequence analysis of invA gene was found conserved in Salmonella serovars and demonstrated 100% homology with closely related serovars of Salmonella.

Conclusion: Invasion gene (invA) was found to be highly conserved in Salmonella Gallinarum and highly similar with closely related serovars. The isolates also contained plasmid-mediated spvC gene indicating possession of virulence plasmid. Keywords: invA, polymerase chain reaction, Salmonella Gallinarum, Salmonella plasmid virulence C, virulence genes.

Keywords: invA, polymerase chain reaction, Salmonella Gallinarum, Salmonella plasmid virulence C, virulence genes.

How to cite this article: Pal S, Dey S, Batabyal K, Banerjee A, Joardar SN, Samanta I, Isore DP (2017) Characterization of Salmonella Gallinarum isolates from backyard poultry by polymerase chain reaction detection of invasion (invA) and Salmonella plasmid virulence (spvC) genes, Veterinary World, 10(7): 814-817.

Received: 07-02-2017 Accepted: 13-06-2017 Published online: 23-07-2017

Corresponding author: Samir Dey E-mail: samirddy@yahoo.co.in

DOI: 10.14202/vetworld.2017.814-817

Copyright: Pal, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.