Effects of hyperbaric therapy on liver morphofunctional of rabbits (Oryctolagus cuniculus) after hind limb ischemia-reperfusion injury

Vet World Vol.10 November-2017 Article-10

Research Article

Veterinary World, 10(11): 1337-1342

https://doi.org/10.14202/vetworld.2017.1337-1342

Bambang Sektiari Lukiswanto¹, Wiwik Misaco Yuniarti¹, and Y. Yosis Motulo²

1. Department of Veterinary Clinic, Faculty of Veterinary Medicine, Universitas Airlangga, Jl. Mulyorejo, Kampus C, Surabaya 60115, Indonesia.
2. Department of Surgery, Division of Thoracic, Cardiac, and Vascular Surgery, Dr. Soetomo General Hospital, Surabaya 60115, Indonesia.

Background and Aim: The objective of this research was to study and to prove the effectiveness of hyperbaric oxygen therapy (HBOT) starting time on liver morphofunctional changes after ischemia-reperfusion in the hind limb of rabbits.

Materials and Methods: This research used a complete randomized design with 4 groups and 6 repetitions on each. After 6 h artery femoral is ligation, reperfusion was performed for 100 min (G1), HBOT for 90 min after 10 min reperfusion (G2), 10 min reperfusion (G3), and HBOT 90 min after 60 min reperfusion (G4). Then, all of the rabbits were sacrificed. The liver and blood were taken for histopathological changes examination as well as for measuring the level of aspartate aminotransferase (AST) and alanine aminotransferase (ALT). The statistical test using Kruskal-Wallis and Mann-Whitney showed that the score of degeneration, necrosis, and portal inflammation in groups without HBOT (G1 and G3) were not significantly different, as well as in group with HBOT (G2 and G4) (p>0.05). However, the scores of histopathological changes in G1 and G3 were significantly different from those in G2 and G4 (p<0.05). The levels of AST and ALT in the groups without hyperbaric therapy (G1 and G3) were not significantly different from those in the groups treated with hyperbaric therapy (G2 and G4) (p>0.05).

Results: Hind limb ischemia injury reperfusion can trigger damage for liver morphology, but not lead to liver dysfunction. Reperfusion can trigger increased activity of neutrophils, while neutrophil infiltration in the organ will lead to dysfunction. HBOT can inhibit the activity of neutrophils and the dysfunction of organs caused by ischemic reperfusion.

Conclusion: HBOT for 90 min, both 10 and 60 min after the reperfusion, can protect hepatocytes from damage. Keywords: hyperbaric oxygen therapy, ischemia, liver, morphofunctional, reperfusion.

Keywords: hyperbaric oxygen therapy, ischemia, liver, morphofunctional, reperfusion.

How to cite this article: Lukiswanto BS, Yuniarti WM, Motulo YY (2017) Effects of hyperbaric therapy on liver morphofunctional of rabbits (Oryctolagus cuniculus) after hind limb ischemia-reperfusion injury, Veterinary World, 10(11): 1337-1342.

Received: 11-06-2017 Accepted: 18-10-2017 Published online: 14-11-2017

Corresponding author: Bambang Sektiari Lukiswanto E-mail: bamsekti@yahoo.com

DOI: 10.14202/vetworld.2017.1337-1342

Copyright: Lukiswanto, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.