Molecular analysis of genome segment-3 of bluetongue virus serotype 12 isolates from Haryana

Vet World Vol.10 November-2017 Article-18

Research Article

Veterinary World, 10(11): 1389-1393

https://doi.org/10.14202/vetworld.2017.1389-1393

Anita Dalal¹, Sushila Maan², Nitish Bansal², Vinay Kumar², Aman Kumar², Narender Singh Maan^3,2, and Naresh Kumar Kakker¹

1. Department of Veterinary Microbiology, College of Veterinary Sciences, Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar - 125 004, Haryana, India.
2. Department of Animal Biotechnology, College of Veterinary Sciences, Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar - 125 004, Haryana, India.
3. Department of Animal Nutrition, College of Veterinary Sciences, Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar - 125 004, Haryana, India.

Background and Aim: The present study was designed to characterize the genome segment 3 (Seg-3) of bluetongue virus (BTV) serotype 12 isolates from different outbreaks of Bluetongue disease in Haryana, India.

Materials and Methods: Blood and swab samples were collected from goat and sheep suspected to be suffering of BT from different outbreaks from Gurugram, Sirsa, Hisar, and Karnal districts of Haryana. The samples were grown in insect and mammalian cell lines. After preliminary identification, serotyping was done using BTV type-specific quantitative reverse transcription-polymerase chain reaction (qRT-PCR) assays. Sequencing was performed using terminal and walking internal primers specific for Seg-3 on ABI Capillary Sequencer 3130 using a "BigDye cycle sequencing kit." The obtained sequence data were analyzed with various bioinformatic tools.

Results: Real-time PCR results confirmed the samples to be positive for BTV-12. The Seg-3 of Indian isolates was most closely related to that of a south Indian isolate of BTV-12 from Andhra Pradesh (KC662614) with 97% nucleotide identity.

Conclusion: The study confirmed the circulation of BTV-12 in Haryana, India. The variations shown in genome Seg-3 of BTV-12 isolates may have some significance and need to be further explored. Keywords: bluetongue, bluetongue virus-12, genome segment-3, Haryana, real time, serotype, sequencing.

Keywords: bluetongue, bluetongue virus-12, genome segment-3, Haryana, real time, serotype, sequencing.

How to cite this article: Dalal A, Maan S, Bansal N, Kumar V, Kumar A, Maan NS, Kakker NK (2017) Molecular analysis of genome segment-3 of bluetongue virus serotype 12 isolates from Haryana, Veterinary World, 10(11): 1389-1393.

Received: 15-07-2017 Accepted: 25-10-2017 Published online: 28-11-2017

Corresponding author: Naresh Kumar Kakker E-mail: nkmksk@rediffmail.com

DOI: 10.14202/vetworld.2017.1389-1393

Copyright: Dalal, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.