Vet World Vol.10 November-2017 Article-7
Research Article
Veterinary World, 10(11): 1324-1328
https://doi.org/10.14202/vetworld.2017.1324-1328
The phylogenetics of Leucocytozoon caulleryi infecting broiler chickens in endemic areas in Indonesia
2. Department of Clinical Science, Faculty of Veterinary Medicine, Universitas Airlangga, Jl. Mulyorejo, Kampus C Unair, Surabaya, 60115, Indonesia.
Background and Aim: The objective of this research was to determine the species and strains of Leucocytozoon caulleryi and study the phylogenetics of L. caulleryi of broiler chickens in endemic areas in Indonesia.
Materials and Methods: Blood samples were collected from broiler chickens originated from endemic area in Indonesia, i.e., Pasuruan, Lamongan, Blitar, Lumajang, Boyolali, Purwokerto, and Banjarmasin in 2017. Collected blood was used for microscopic examination, sequencing using BLAST method to identify the nucleotide structure of cytochrome b (cyt b) gene that determines the species, and the phylogenetics analysis of L. caulleryi that infected broiler chickens in endemic areas in Indonesia, using Mega 5 software.
Results: The results showed that Plasmodium sp. and L. caulleryi were infected broiler chickens in endemic areas in Indonesia. L. caulleryi in one area had very close phylogenetic relations with those in other areas. The genetic distance between L. caulleryi taxa from various endemic areas is very close (<5%).
Conclusion: There is a very close phylogenetics among strains of L. caulleryi that infected broiler chickens in various endemic areas in Indonesia. Keywords: broiler chickens, cytochrome b gene, endemic, Indonesia, Leucocytozoon caulleryi.
Keywords: broiler chickens, cytochrome b gene, endemic, Indonesia, Leucocytozoon caulleryi.
How to cite this article: Suprihati E, Yuniarti WM (2017) The phylogenetics of Leucocytozoon caulleryi infecting broiler chickens in endemic areas in Indonesia, Veterinary World, 10(11): 1324 1328.
Received: 13-06-2017 Accepted: 13-10-2017 Published online: 11-11-2017
Corresponding author: Wiwik Misaco Yuniarti E-mail: wiwikmisaco@yahoo.com
DOI: 10.14202/vetworld.2017.1324-1328
Copyright: Suprihati and Yuniarti, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.