Vet World   Vol.10   September-2017  Article-18

Repertoire of noncoding RNAs in corpus luteum of early pregnancy in buffalo (Bubalus bubalis)

Background and Aim: The present study was designed to identify other noncoding RNAs (ncRNAs) in the corpus luteum (CL) during early pregnancy in buffalo.

Materials and Methods: For this study, CL (n=2) from two buffalo gravid uteri, obtained from the slaughter house, was transported to laboratory after snap freezing in liquid nitrogen (-196°C). The stage of pregnancy was determined by measuring the crown-rump region of the fetus. This was followed by isolation of RNA and deep sequencing. Post-deep sequencing, the obtained reads were checked and aligned against various ncRNA databases (GtRNA, RFAM, and deep guide). Various parameters, namely, frequency of specific ncRNAs, length, mismatch, and genomic location target in several model species were deciphered.

Results: Frequency of piwi-interacting RNAs (piwi-RNAs), having target location in rodents and human genomes, were significantly higher compared to other piwi-RNAs and ncRNAs. Ribosomal RNAs (rRNAs) deduced had nucleotides (nts) ranging from 17 to 50 nts, but the occurrence of small length rRNAs was more than lengthier fragments. The target on 16S rRNA species confirms the conservation of 16S rRNA across species. With respect to transfer RNA (tRNA), the abundantly occurring tRNAs were unique with no duplication. Small nucleolar RNAs (snoRNAs), identified in this study, showed a strong tendency for coding box C/D snoRNAs in comparison to H/ACA snoRNAs. Regulatory and evolutionary implications of these identified ncRNAs are yet to be delineated in many species, including buffaloes.

Conclusion: This is the first report of identification of other ncRNAs in CL of early pregnancy in buffalo. Keywords: buffalo, corpus luteum, noncoding RNA, pregnancy.

Keywords: buffalo, corpus luteum, noncoding RNA, pregnancy.

How to cite this article: Jerome A, Thirumaran SMK, Kala SN (2017) Repertoire of noncoding RNAs in corpus luteum of early pregnancy in buffalo (Bubalus bubalis), Veterinary World, 10(9): 1129-1134.

Received: 07-07-2017  Accepted: 24-08-2017     Published online: 25-09-2017

Corresponding author: A. Jerome   E-mail: jerome210982@gmail.com

DOI: 10.14202/vetworld.2017.1129-1134

Copyright: Jerome, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.