Detection of Toxoplasma gondii in cat's internal organs by immunohistochemistry methods labeled with-[strept] avidin-biotin

Vet World Vol.10 September-2017 Article-4

Research Article

Veterinary World, 10(9): 1035-1039

https://doi.org/10.14202/vetworld.2017.1035-1039

Muhammad Hanafiah¹, Raden Wisnu Nurcahyo², Rika Yuniar Siregar³, Joko Prastowo², Sri Hartati⁴, Bambang Sutrisno⁵, and Dwinna Aliza⁶

1. Parasitology Laboratory, Faculty of Veterinary Medicine, Syiah Kuala University, Banda Aceh, Indonesia.
2. Department of Parasitology, Faculty of Veterinary Medicine, Gadjah Mada University, Yogyakarta, Indonesia.
3. Veterinary Public Health of Laboratory, Diseases Investigation Center, Wates, Yogyakarta, Indonesia.
4. Department of Clinic, Faculty of Veterinary Medicine, Gadjah Mada University, Yogyakarta, Indonesia.
5. Department of Pathology, FKH UGM, Sekip Unit II Yogyakarta 55281, Yogyakarta, Indonesia.
6. Pathology Laboratory, Faculty of Veterinary Medicine, Syiah Kuala University Banda Aceh, Indonesia.

Background and Aim: The aims of the study are to detect the presence of Toxoplasma gondii antigen and to determine its distribution location in several organs of domestic cat using immunohistochemistry (IHC) method with Labeled-[Strept] Avidin-Biotin (LAB-SA).

Materials and Methods: Four domestic cats aged 1-2 years were used as sample in this research. The sample divided into two groups with two cats each. Cats in Group I were positive Toxoplasma based on serologically screening test, while cats in Group II were orally infected with 1x106 Toxoplasma oocyst. All samples then necropsied, and the organs including brain, liver, kidney, duodenum, jejunum, ileum, lungs, and spleen were collected for IHC method with LAB-SA. Result: The result showed that Toxoplasma antigens were detected in ileum of both serologically positive domestic cat and the experimentally infected cats. Toxoplasma was also observed in kidney of serologically positive domestic cat. In the serologically positive domestic cat, necrotic lesions were found on ileum, kidney, and liver, whereas in experimentally infected cat, the lesion was only found on ileum.

Conclusion: The presence of Toxoplasma antigen is successfully detected in several organs of domestic cat using IHC method with the LAB-SA. Keywords: cat, immunohistochemistry, labeled-[strept] avidin-biotin, Toxoplasma gondii.

Keywords: cat, immunohistochemistry, labeled-[strept] avidin-biotin, Toxoplasma gondii.

How to cite this article: Hanafiah M, Nurcahyo RW, Siregar RY, Prastowo J, Hartati S, Sutrisno B, Aliza D (2017) Detection of Toxoplasma gondii in cat's internal organs by immunohistochemistry methods labeled with-[strept] avidin-biotin, Veterinary World, 10(9): 1035-1039.

Received: 22-03-2017 Accepted: 03-08-2017 Published online: 06-09-2017

Corresponding author: Muhammad Hanafiah E-mail: hanafi_2015@unsyiah.ac.id

DOI: 10.14202/vetworld.2017.1035-1039

Copyright: Hanafiah, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.