Seroprevalence of brucellosis in small ruminants in organized and unorganized sectors of Gujarat state, India

Vet World Vol.11 August-2018 Article-2

Research Article

Veterinary World, 11(8): 1030-1036

https://doi.org/10.14202/vetworld.2018.1030-1036

A. Kanani¹, S. Dabhi¹, Y. Patel², V. Chandra¹, O. R. Vinodh Kumar³, and R. Shome⁴

1. Office of Deputy Director of Animal Husbandry, F.M.D. Typing Scheme, Polytechnic Campus, Ambawadi, Ahmedabad - 380 015, Gujarat, India.
2. Department of Biomedical Technology, University School of Science, Gujarat University, Ahmedabad, Gujarat.
3. ICAR-National Institute of Veterinary Epidemiology and Disease Informatics (ICAR-NIVEDI), Yelahanka, Bengaluru - 560 064, Karnataka, India.
4. Division of Epidemiology, ICAR-Indian Veterinary Research Institute, Izatnagar, Bareilly - 243 122, Uttar Pradesh, India.

Background and Aim: The present study aimed to study the seroprevalence of brucellosis in small ruminants of Gujarat state, India, using Rose Bengal Plate test (RBPT) and indirect enzyme-linked immunosorbent assay (iELISA).

Materials and Methods: A total of 2444 sera samples (675 sheep and 1769 goat) from unorganized sector and 1310 sera samples (861 sheep and 449 goat) from seven organized farms were collected for brucellosis screening.

Results: In unorganized sector, 23.70% sheep (160/675) and 15.99% goat (283/1769) were positive by RBPT and 24.44% sheep (165/675) and 17.24% goat (305/1769) by iELISA. The organized sector samples showed higher seroprevalence in goat (7.79 %, 35/449) than sheep (4.06 %, 35/861) by RBPT. Similarly, in iELISA, goat samples showed a higher seroprevalence (9.35%, 42/449) compared to sheep (7.50%, 65/861). The diagnostic sensitivity and specificity of RBPT with ELISA were 88.69% and 99.65%, respectively, and showed a significant difference (p≤0.0001). The Chi-square analysis revealed a significant difference in seroprevalence between sectors (p≤0.01) and species (p≤0.01).

Conclusion: The seroprevalence of brucellosis in small ruminants of Gujarat was investigated and showed a higher prevalence of brucellosis and warrants the implementation of proper preventive measures. Keywords: brucellosis, Gujarat, indirect enzyme-linked immunosorbent assay, Rose Bengal Plate test, seroprevalence, small ruminants.

Keywords: brucellosis, Gujarat, indirect enzyme-linked immunosorbent assay, Rose Bengal Plate test, seroprevalence, small ruminants.

How to cite this article: Kanani A, Dabhi S, Patel Y, Chandra V, Vinodh Kumar OR, Shome R (2018) Seroprevalence of brucellosis in small ruminants in organized and unorganized sectors of Gujarat state, India, Veterinary World, 11(8): 1030-1036.

Received: 07-02-2018 Accepted: 12-06-2018 Published online: 01-08-2018

Corresponding author: R. Shome E-mail: rajeswarishome@gmail.com

DOI: 10.14202/vetworld.2018.1030-1036

Copyright: Kanani, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.