Vet World Vol.11 February-2018 Article-22
Research Article
Veterinary World, 11(2): 227-230
https://doi.org/10.14202/vetworld.2018.227-230
Interspecies comparison of probiotics isolated from different animals
2. Department of Parasitology and Animal Diseases, Division of Veterinary Research, National Research Centre, 12622 Dokki, Giza, Egypt.
Background and Aim: The aim of the current study was to isolate and identify naturally occurring probiotic Lactobacillus species in different animals with the different environmental background including fish, and farm animals to investigate interspecies differences in probiotics on the species level.
Materials and Methods: A total of 44 fecal and milk samples were collected under aseptic conditions from cattle, buffalo, camel, sheep, goats, and fish. The samples were cultured, and the isolated strains were confirmed biochemically and molecularly using 16S rRNA multiplex polymerase chain reaction (PCR) analysis following DNA extraction from the bacterial isolates.
Results: A total of 31 isolates identified as lactobacilli were isolated from cattle milk, goat feces, sheep feces, fish feces, buffalo milk, camel milk, and goats' milk. Lactobacillus species were identified based on the size of the PCR product. The results showed that different species were different in their lactobacilli content. At the same time, there were some differences between individuals of the same species.
Conclusion: The diversity of probiotic strains isolated from different animal species implies different types of benefits to the host. Although it would be both money - and time-consuming research, discovering the benefit of each of these strains may provide very important information for the health of both human and animal. Furthermore, transferring these beneficial effects either to individuals within the same species or between different species would be of great importance. Keywords: Lactobacillus, multiplex polymerase chain reaction, probiotics.
Keywords: Lactobacillus, multiplex polymerase chain reaction, probiotics.
How to cite this article: Abdou AM, Hedia RH, Omara ST, Mahmoud MAE, Kandil MM, Bakry MA (2018) Interspecies comparison of probiotics isolated from different animals, Veterinary World, 11(2): 227-230.
Received: 17-11-2017 Accepted: 16-01-2018 Published online: 21-02-2018
Corresponding author: Amr M. Abdou E-mail: amrkheir@yahoo.com
DOI: 10.14202/vetworld.2018.227-230
Copyright: Abdou, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.