Vet World Vol.11 June-2018 Article-11
Research Article
Veterinary World, 11(6): 800-808
https://doi.org/10.14202/vetworld.2018.800-808
The effectiveness of platelet-rich plasma on the skin wound healing process: A comparative experimental study in sheep
2. Biotechnology's Laboratory of the Bioactive Molecules and the Cellular Physiopathology, University of Batna 2, Batna, Algeria.
3. Department of Veterinary Science, Institute of Agronomic and Veterinary Sciences, University of Batna 1, Batna, Algeria.
Background and Aim: The therapeutic evaluation of the biological effect of platelet-rich plasma (PRP) used as a surgical adjunct to maintain the inflammatory process and to potentiate tissue healing, make the subject of recent research in regenerative medicine. This study was designed to evaluate the healing activity of PRP by its topical application on the skin experimentally injured in a sheep model.
Materials and Methods: The study was conducted on 9 adult and clinically healthy males sheep. PRP was obtained by a protocol of double centrifugation of whole blood from each animal. After sterile skin preparation, full-thickness excisional wounds (20 mm x 20 mm) were created on the back of each animal. The animals were randomly divided into three equal groups of three sheep for each. In Group I, the wounds were treated with PRP, in Group II; wounds were treated with Asiaticoside; in Group III, wounds were treated with saline solution. The different treatments were administered topically every 3 days. Morphometric measurements of the contraction surface of the wounds and histopathological biopsies were carried out at the 3rd, 7th, 14th, 21st, and 28th days of healing.
Results: The results of the morphometric data obtained revealed that it was significant differences recorded at the 7th and 14th day of healing in favor for animals of Group I. Semi-quantitative histopathological evaluation showed that PRP reduces inflammation during 3 first days post-surgical and promotes epithelialization in 3 weeks of healing.
Conclusion: We concluded that topical administration of PRP obtained by double centrifugation protocol could potentially improve the skin healing process in sheep. Keywords: healing, platelet-rich plasma, sheep, skin.
Keywords: healing, platelet-rich plasma, sheep, skin.
How to cite this article: Badis D, Omar B (2018) The effectiveness of platelet-rich plasma on the skin wound healing process: A comparative experimental study in sheep, Veterinary World, 11(6): 800-808.
Received: 24-03-2018 Accepted: 22-05-2018 Published online: 14-06-2018
Corresponding author: Daikh Badis E-mail: badis.daikh@gmail.com
DOI: 10.14202/vetworld.2018.800-808
Copyright: Badis and Omar, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.