Vet World Vol.11 June-2018 Article-13
Research Article
Veterinary World, 11(6): 819-823
https://doi.org/10.14202/vetworld.2018.819-823
Humoral and cellular immune response induced by antigenic protein of Sarcoptes scabiei var. caprae
2. Department of Veterinary Clinic, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya, Indonesia.
3. Study Programme of Animal Health, Faculty of Vocation, Universitas Airlangga, Surabaya, Indonesia.
Background and Aim: Scabies is one of the most important diseases in goats and caused by a complex hypersensitivity process that involves both humoral and cell-mediated immune responses. This phenomenon shows that the variety of Sarcoptes scabiei has different characteristics of specific antigenic protein or different immune-dominant. This research aims to detect the humoral and cellular immune response of rabbits which were immunized with the protein of S. scabiei var. caprae.
Materials and Methods: This research was done as follows, identification and collection of Sarcoptes scabiei var. caprae from goat infected with scabies, separation of protein antigen from S. scabiei mites with ultrasonic sonicator, measurement of protein content with spectrophotometry, rabbit injection with 500 μg dose of antigen protein which was repeated 5 times (5x booster) every 2 weeks. Measurement of IgG titer using indirect ELISA, whereas to detect the expression of cellular immune response (TLR-9, CD4, and CD8) using Direct Immunofluorescence assay.
Results: Based on the statistical analysis, it showed that there was a significant enhancement (p<0.05) for optical density value or antibody titer and cellular immune response was shown by TLR-9, CD4, and CD8 expression in rabbit T lymphocytes which appear yellow to green fluorescent color using fluorescence microscope. The amount of fluorescence T lymphocytes showed a significant difference (p<0.05) between the control and various boosters.
Conclusion: Antigenic protein of S. scabiei var. caprae contains ligands, which are involved in the pathogen-associated molecular pattern that has an ability to induce humoral and cellular immune response in rabbit. Specifically, that TLR-9 is not only involved in innate immunity but also in adaptive immunity and can be used as alternative adjuvant development research. Keywords: antigenic protein, CD4, CD8, immunoglobulin G, Sarcoptes scabiei var. caprae, toll-like receptor-9.
Keywords: antigenic protein, CD4, CD8, immunoglobulin G, Sarcoptes scabiei var. caprae, toll-like receptor-9.
How to cite this article: Lastuti NDR, Yuniarti WM, Hastutiek P, Suwanti LT, Chrismanto D (2018) Humoral and cellular immune response induced by antigenic protein of Sarcoptes scabiei var. caprae, Veterinary World, 11(6): 819-823.
Received: 17-01-2018 Accepted: 14-05-2018 Published online: 19-06-2018
Corresponding author: Wiwik Misaco Yuniarti E-mail: wiwikmisaco@yahoo.com
DOI: 10.14202/vetworld.2018.819-823
Copyright: Lastuti NDR, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.