Research Article | 16 Mar 2018

Prevalence and characterization of Panton-Valentine leukocidin-positive Staphylococcus aureus in bovine milk in Jabalpur district of Madhya Pradesh, India

Neeraj Shrivastava1, Varsha Sharma1, Arpita Shrivastav2, Anju Nayak1, and Ajay Kumar Rai1Show more
VETERINARY WORLD | pg no. 316-320 | Vol. 11, Issue 3 | DOI: 10.14202/vetworld.2018.316-320
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Abstract

          
Background and Aim: The study aimed to investigate the Panton-Valentine leukocidin (PVL)-positive Staphylococcus aureus in bovine milk due to its public health significance.
Materials and Methods: A total of 400 milk samples of bovines taken from different dairy farms and outlets of Jabalpur were screened for the S. aureus and methicillin-resistant S. aureus (MRSA). The strains were tested for the PVL gene and antimicrobial sensitivity toward 10 different classes of antimicrobial agents. The PVL-positive S. aureus strains were further characterized by staphylococcal protein A or spa typing.
Results: The prevalence of PVL-positive S. aureus was 10.53%. All the isolates positive for the PVL were resistant to methicillin, while the methicillin-sensitive S. aureus isolates were negative for the PVL. Five different spa types were found.
Conclusion: The presence of PVL-positive MRSA in bovine milk close to consumer poses a potential public health risk to the community. Keywords: bovine milk, methicillin-resistant Staphylococcus aureus, Panton-Valentine leukocidin.
          
Keywords: bovine milk, methicillin-resistant Staphylococcus aureus, Panton-Valentine leukocidin.