Vet World Vol.11 March-2018 Article-14
Research Article
Veterinary World, 11(3): 342-347
https://doi.org/10.14202/vetworld.2018.342-347
Antibacterial activity of fig leaf (Ficus carica Linn.) extract against Enterococcus faecalis and its cytotoxicity effects on fibroblast cells
2. Undergraduate Student of Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia.
Background and Aim: This study aimed to determine the minimum bactericidal concentration (MBC) of fig leaf (F. carica Linn.) extract against E. faecalis and its cytotoxicity on fibroblast cells in vitro.
Materials and Methods: A serial dilution method was used to determine the MBC of fig leaf extract on E. faecalis which grown on nutrient agar media. Inoculation was carried out at concentrations that suspected minimum inhibitory concentration (MIC), MBC, concentration between MIC and MBC, and control groups on different nutrient agar. MIC and MBC of fig leaf extract against E. faecalis were known by counting the growth of bacteria colonies on nutrient agar media in CFU/ml. The cytotoxicity of MIC and MBC of the extract acquired were tested using 3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide assay, and the results were read using an ELISA reader. Data of E. faecalis colonies were analyzed using Kruskal-Wallis and Mann-Whitney test.
Results: The result showed a significant difference among the groups (p<0.05). Fig leaf extract at a concentration of 50% showed no bacterial growth, and cell viability at this concentration was 77.7%.
Conclusion: Fig leaf extract has antibacterial effect on E. faecalis with MBC at 50% and not cytotoxic to fibroblast cells. Keywords: Enterococcus faecalis, fig leaf (Ficus carica Linn.) extract, minimum bactericidal concentration, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay.
Keywords: Enterococcus faecalis, fig leaf (Ficus carica Linn.) extract, minimum bactericidal concentration, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay.
How to cite this article: Nirwana I, Rianti D, Soekartono RH, Listyorini RD, Basuki DP (2018) Antibacterial activity of fig leaf (Ficus carica Linn.) extract against Enterococcus faecalis and its cytotoxicity effects on fibroblast cells, Veterinary World, 11(3): 342-347.
Received: 13-11-2017 Accepted: 20-02-2018 Published online: 20-03-2018
Corresponding author: Intan Nirwana E-mail: intan-n@fkg.unair.ac.id
DOI: 10.14202/vetworld.2018.342-347
Copyright: Nirwana, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.