Vet World Vol.11 March-2018 Article-15
Research Article
Veterinary World, 11(3): 348-354
https://doi.org/10.14202/vetworld.2018.348-354
Virulence gene profiling of porcine Pasteurella multocida isolates of Assam
2. Department of Microbiology, College of Veterinary Science, AAU, Khanapara, Guwahati - 781 022, Assam, India.
3. Department of Veterinary Epidemiology and Preventive Medicine, CVSc, CAU, Aizawl, Mizoram, India.
4. Department of Veterinary Public Health, College of Veterinary Science, AAU, Khanapara, Guwahati - 781 022, Assam, India.
Background and Aim: The present study was conducted to detect and identify the virulence genes in Pasteurella multocida isolates of porcine origin from Assam.
Materials and Methods: A total of 21 porcine P. multocida isolates were subjected to capsular typing and detection of virulence-associated genes (pfhA, tbpA, hgbB, toxA, oma87, ompH, and nanB) using various polymerase chain reaction (PCR) methods reported elsewhere. Further, pathogenicity of the porcine isolates of P. multocida was studied in mice. For each strain of P. multocida selected for pathogenicity trial, the group of mice was injected intraperitoneally (i/p) with 0.1 ml of the inoculum prepared from respective field isolates, containing 109 organisms per ml.
Results: Capsular typing of the isolates by multiplex PCR showed two capsular types, type A (66.66%) and type D (33.33%). All the isolates were positive for outer membrane protein genes, oma87 and ompH genes. Iron acquisition genes, tbpA and hgbB, were detected in 14.28% and 19.04% of the isolates. The dermonecrotoxin encoding gene, toxA, was present in 23.80% of the isolates. Filamentous hemagglutinin encoding gene, pfhA, was detected in 28.57%. The virulence gene distribution pattern of the isolates indicates the important role of the genes in disease pathogenesis.
Conclusion: From the present study, it can be concluded that toxA gene is an important marker gene for defining the pathogenic potential of P. multocida strains in swine. Keywords: capsular type, Pasteurella multocida, porcine, virulence-associated genes.
Keywords: capsular type, Pasteurella multocida, porcine, virulence-associated genes.
How to cite this article: Devi LB, Bora DP, Das SK, Sharma RK, Mukherjee S, Hazarika RA (2018) Virulence gene profiling of porcine Pasteurella multocida isolates of Assam, Veterinary World, 11(3): 348-354.
Received: 16-08-2017 Accepted: 19-02-2018 Published online: 21-03-2018
Corresponding author: Durlav Prasad Bora E-mail: drdpbora@gmail.com
DOI: 10.14202/vetworld.2018.348-354
Copyright: Devi, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.